Assessment of an immunoturbidimetric method for measuring equine serum haptoglobin concentrations.
Abstract: Serum haptoglobin was measured by immunoturbidity in Thoroughbreds stabled in three Newmarket yards for nine months. The mean serum haptoglobin value for horses housed in Stable 1 was 1.43 +/- 0.68 g/litre, similar to values in grazing adult ponies. The mean monthly haptoglobin values remained constant. For horses in Stable 2 there was an increase in serum haptoglobin values in June and July whereas, in Stable 3, the increases early in the training season were associated with an increase in serum viral titres. In equine serum, haptoglobin values estimated in g/litre by immunoturbidimetry were twice the haemoglobin binding capacity (HbBC). The correlation coefficient, between the methods, exceeded 0.96. Surgical interference caused a 2- to 3-fold increase in serum haptoglobin, with levels peaking between three and five days post surgery. The concomitant presence of haematomata following surgical interference (castration) resulted in the disappearance of haptoglobin from the circulation. There was no significant increase in serum haptoglobin levels following vaccination of Thoroughbreds against influenza and tetanus. However inhalation of influenza virus by vaccinated and non-vaccinated ponies resulted in a 2- to 3-fold increase in both groups of ponies, peaking seven to 10 days post infection. Serum haptoglobin proved to be a useful indicator of infection/inflammation and haemolytic disease in the horse and could be used to monitor the health status of stabled Thoroughbreds.
Publication Date: 1991-01-01 PubMed ID: 1849813DOI: 10.1111/j.2042-3306.1991.tb02716.xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article details a study on measuring equine serum haptoglobin concentrations using an immunoturbidimetric method, with factors such as different stables, surgery, and vaccinations being tested for their effects on the haptoglobin levels.
Assessment of Immunoturbidimetric Method
- The researchers used immunoturbidity to measure serum haptoglobin in Thoroughbred horses from three different Newmarket yards over nine months.
- The mean serum haptoglobin value for horses in Stable 1 was 1.43 +/- 0.68 g/litre, which is similar to values found in adult grazing ponies, and these values remained fairly constant over time.
Impact of Various Factors on Haptoglobin Levels
- Horses in Stable 2 showed an increase in serum haptoglobin values in June and July, while those in Stable 3 had increases early in the training season that correlated with an increase in serum viral titres.
- Surgical interference caused haptoglobin concentrations to increase 2- to 3-fold, peaking between three and five days after surgery.
- The presence of haematomata following castration resulted in the disappearance of haptoglobin from the horse’s circulation.
- There was no significant rise in serum haptoglobin levels after the horses were vaccinated against influenza and tetanus.
- In contrast, inhaling the influenza virus caused a 2- to 3-fold increase in haptoglobin levels in both vaccinated and non-vaccinated ponies, peaking seven to 10 days after infection.
Conclusion and Implications
- The value of haptoglobin in equine serum as estimated by immunoturbidity was twice the haemoglobin binding capacity.
- The methods showed a correlation coefficient exceeding 0.96, indicating high reliability.
- The researchers concluded that serum haptoglobin is a useful indicator of infection, inflammation, and haemolytic disease in horses.
- It could therefore be utilised to monitor the health status of stabled Thoroughbreds, providing useful information for those managing and caring for these horses.
Cite This Article
APA
Kent JE, Goodall J.
(1991).
Assessment of an immunoturbidimetric method for measuring equine serum haptoglobin concentrations.
Equine Vet J, 23(1), 59-66.
https://doi.org/10.1111/j.2042-3306.1991.tb02716.x Publication
Researcher Affiliations
- Department of Pre-clinical Veterinary Studies, Edinburgh University, Summerhall, UK.
MeSH Terms
- Animals
- Blood Preservation
- Female
- Haptoglobins / analysis
- Hemoglobins / metabolism
- Horse Diseases / blood
- Horses / blood
- Male
- Nephelometry and Turbidimetry
- Orthomyxoviridae Infections / blood
- Orthomyxoviridae Infections / veterinary
- Predictive Value of Tests
- Reference Values
- Seasons
Citations
This article has been cited 7 times.- Hassanpour A, Moghaddam S. Evaluation of serum concentration of acute-phase proteins (haptoglobin and serum amyloid A) in the affected Arabian foals with rhodococcosis.. Vet Med Sci 2023 Jan;9(1):144-149.
- El-Deeb WM, Buczinski S. The diagnostic and prognostic importance of oxidative stress biomarkers and acute phase proteins in Urinary Tract Infection (UTI) in camels.. PeerJ 2015;3:e1363.
- Barbé F, Atanasova K, Van Reeth K. Cytokines and acute phase proteins associated with acute swine influenza infection in pigs.. Vet J 2011 Jan;187(1):48-53.
- Tecles F, Subiela SM, Petrucci G, Panizo CG, Cerón JJ. Validation of a commercially available human immunoturbidimetric assay for haptoglobin determination in canine serum samples.. Vet Res Commun 2007 Jan;31(1):23-36.
- Hultén C, Sandgren B, Skiöldebrand E, Klingeborn B, Marhaug G, Forsberg M. The acute phase protein serum amyloid A (SAA) as an inflammatory marker in equine influenza virus infection.. Acta Vet Scand 1999;40(4):323-33.
- Eurell TE, Wilson DA, Baker GJ. The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.. Can J Vet Res 1993 Jan;57(1):42-4.
- Milne EM. An improved method for the study of equine haptoglobin heterogeneity.. Vet Res Commun 1990;14(6):433-9.
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