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Home / Equine Research Bank / J Vet Diagn Invest / Assessment of platelet biology in equine patients with syste...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2020; 33(2); 300-307; doi: 10.1177/1040638720983791

Assessment of platelet biology in equine patients with systemic inflammatory response syndrome.

Abstract: In addition to maintaining hemostasis, platelets have an important role in modulating innate and adaptive immune responses. A low platelet count has been found to be a negative prognostic factor for survival in humans and horses with critical illnesses, such as sepsis or systemic inflammatory response syndrome (SIRS). Decreased platelet aggregation, caused by in vivo activation, has been found in human patients with severe sepsis. In our prospective controlled study, we assessed platelet biology in blood samples from 20 equine SIRS cases and 120 healthy control horses. Platelet variables such as platelet count, large platelet count, clumps, plateletcrit, mean platelet volume, and mean platelet component concentration were analyzed by laser flow cytometry (Advia 2120) from KEDTA blood and from citrate blood. Hirudin blood samples were analyzed by impedance aggregometry (Multiplate analyzer; Roche) for platelet aggregation, including spontaneous aggregation and aggregation by 4 different agonists: adenosine diphosphate (ADPtest), ADP + prostaglandin E1 (ADPtestHS), arachidonic acid (ASPItest), and collagen (COLtest). SIRS cases had significantly lower platelet counts in KEDTA blood ( < 0.0001) compared to control horses. There were no significant differences in aggregation values between SIRS cases and controls. Non-surviving SIRS horses did not have statistically significant lower platelet counts or lower aggregation values for COLtest, ADPtest, or ADPtestHS compared to surviving SIRS horses, although 5 non-survivors were thrombocytopenic.
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Publication Date: 2020-12-22 PubMed ID: 33353486PubMed Central: PMC7944423DOI: 10.1177/1040638720983791Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studies platelet activity in horses with systemic inflammatory response syndrome (SIRS) and notes a significant drop in platelet numbers in affected horses. However, no noteworthy changes in aggregation or increased mortality rate among stricken horses lacking in platelet or aggregation metrics were found.

Methodology

  • The study was designed as a prospective controlled study, with the test subjects being horses diagnosed with systemic inflammatory response syndrome (SIRS). The control group consisted of healthy, non-SIRS horses.
  • Several variables including platelet count, large platelet count, clumps, plateletcrit, mean platelet volume, and mean platelet component concentration were evaluated using laser flow cytometry.
  • Both KEDTA and citrate blood samples were used to better understand the platelet biology.
  • The researchers also added a variety of platelet-aggregating substances (aggregation by four different agonists: adenosine diphosphate (ADPtest), ADP + prostaglandin E1 (ADPtestHS), arachidonic acid (ASPItest), and collagen (COLtest)) to the blood samples to evaluate platelet reaction and aggregation.
  • The study also considered the survival rate among SIRS affected horses in relation to their platelet count and aggregation values.

Findings

  • The most significant finding was a dramatic reduction in platelet count when it came to SIRS diagnosed horses compared to the healthy control subjects.
  • Despite the low count, the study found no significant change in the platelet aggregation properties in SIRS diagnosed horses, even after adding aggregating substances to the blood samples.
  • Furthermore, no significant difference was found in terms of survival rate between horses with a lower than average platelet count or degradation values and those with standard values.

Conclusion

  • The study thus suggests that while SIRS can bring about a marked decrease in the platelet count in horses, it does not significantly affect platelet aggregation nor does a low platelet count indicate higher risk of mortality.
  • The count and aggregation capabilities of platelets in this context, therefore, might not serve as reliable markers of disease severity or prognosis.
  • This research contributes to better understanding how systemic inflammation affects different biological parameters, and can guide further exploration into the mechanisms and consequences of these changes.

Cite This Article

APA
Ehrmann C, Engel J, Moritz A, Roscher K. (2020). Assessment of platelet biology in equine patients with systemic inflammatory response syndrome. J Vet Diagn Invest, 33(2), 300-307. https://doi.org/10.1177/1040638720983791

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 33
Issue: 2
Pages: 300-307

Researcher Affiliations

Ehrmann, Carolin
  • Equine Clinic, Internal Medicine, Department of Veterinary Clinical Science, Justus Liebig University, Giessen, Germany.
Engel, Julia
  • Equine Clinic, Internal Medicine, Department of Veterinary Clinical Science, Justus Liebig University, Giessen, Germany.
Moritz, Andreas
  • Clinical Pathophysiology and Veterinary Clinical Pathology, Department of Veterinary Clinical Science, Justus Liebig University, Giessen, Germany.
Roscher, Katja
  • Equine Clinic, Internal Medicine, Department of Veterinary Clinical Science, Justus Liebig University, Giessen, Germany.

MeSH Terms

  • Animals
  • Blood Platelets / physiology
  • Case-Control Studies
  • Female
  • Germany
  • Horse Diseases / blood
  • Horse Diseases / physiopathology
  • Horses
  • Male
  • Platelet Aggregation
  • Platelet Count / veterinary
  • Prospective Studies
  • Systemic Inflammatory Response Syndrome / blood
  • Systemic Inflammatory Response Syndrome / physiopathology
  • Systemic Inflammatory Response Syndrome / veterinary

Conflict of Interest Statement

Material for the testing of platelet function with the Multiplate analyzer (Roche) was partly provided free of charge from the former manufacturer Dynabyte. This company had no influence on the design of the study; the collection, management, analysis or interpretation of the data; or the preparation of the manuscript. None of the authors of this manuscript has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the manuscript.

References

This article includes 53 references
  1. Adamzik M, Görlinger K, Peters J, Hartmann M. Whole blood impedance aggregometry as a biomarker for the diagnosis and prognosis of severe sepsis.. Crit Care 2012 Oct 22;16(5):R204.
    pmc: PMC3682307pubmed: 23088388doi: 10.1186/cc11816google scholar: lookup
  2. Ahnadi CE, Sabrinah Chapman E, Lépine M, Okrongly D, Pujol-Moix N, Hernández A, Boughrassa F, Grant AM. Assessment of platelet activation in several different anticoagulants by the Advia 120 Hematology System, fluorescence flow cytometry, and electron microscopy.. Thromb Haemost 2003 Nov;90(5):940-8.
    pubmed: 14597991doi: 10.1160/th03-02-0097google scholar: lookup
  3. Akca S, Haji-Michael P, de Mendonça A, Suter P, Levi M, Vincent JL. Time course of platelet counts in critically ill patients.. Crit Care Med 2002 Apr;30(4):753-6.
    pubmed: 11940740doi: 10.1097/00003246-200204000-00005google scholar: lookup
  4. Arman M, Krauel K, Tilley DO, Weber C, Cox D, Greinacher A, Kerrigan SW, Watson SP. Amplification of bacteria-induced platelet activation is triggered by FcγRIIA, integrin αIIbβ3, and platelet factor 4.. Blood 2014 May 15;123(20):3166-74.
    pmc: PMC4023422pubmed: 24642751doi: 10.1182/blood-2013-11-540526google scholar: lookup
  5. Balk RA. Systemic inflammatory response syndrome (SIRS): where did it come from and is it still relevant today?. Virulence 2014 Jan 1;5(1):20-6.
    pmc: PMC3916374pubmed: 24280933doi: 10.4161/viru.27135google scholar: lookup
  6. Bampalis VG, Brantl SA, Siess W. Why and how to eliminate spontaneous platelet aggregation in blood measured by multiple electrode aggregometry.. J Thromb Haemost 2012 Aug;10(8):1710-4.
    pubmed: 22712617doi: 10.1111/j.1538-7836.2012.04819.xgoogle scholar: lookup
  7. Baurand A, Eckly A, Bari N, Léon C, Hechler B, Cazenave JP, Gachet C. Desensitization of the platelet aggregation response to ADP: differential down-regulation of the P2Y1 and P2cyc receptors.. Thromb Haemost 2000 Sep;84(3):484-91.
    pubmed: 11019976
  8. Berkman N, Michaeli Y, Or R, Eldor A. EDTA-dependent pseudothrombocytopenia: a clinical study of 18 patients and a review of the literature.. Am J Hematol 1991 Mar;36(3):195-201.
    pubmed: 1899964doi: 10.1002/ajh.2830360307google scholar: lookup
  9. Bone RC, Balk RA, Cerra FB, Dellinger RP, Fein AM, Knaus WA, Schein RM, Sibbald WJ. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine.. Chest 1992 Jun;101(6):1644-55.
    pubmed: 1303622doi: 10.1378/chest.101.6.1644google scholar: lookup
  10. Bonelli F, Meucci V, Divers TJ, Jose-Cunilleras E, Corazza M, Tognetti R, Guidi G, Intorre L, Sgorbini M. Plasma Procalcitonin Concentration in Healthy Horses and Horses Affected by Systemic Inflammatory Response Syndrome.. J Vet Intern Med 2015 Nov-Dec;29(6):1689-91.
    pmc: PMC4895682pubmed: 26474412doi: 10.1111/jvim.13640google scholar: lookup
  11. Burkett BN, Thomason JM, Hurdle HM, Wills RW, Fontenot RL. Effects of Firocoxib, Flunixin Meglumine, and Phenylbutazone on Platelet Function and Thromboxane Synthesis in Healthy Horses.. Vet Surg 2016 Nov;45(8):1087-1094.
    pubmed: 27731498doi: 10.1111/vsu.12567google scholar: lookup
  12. Carroll CL, Huntington PJ. Body condition scoring and weight estimation of horses.. Equine Vet J 1988 Jan;20(1):41-5.
    pubmed: 3366105doi: 10.1111/j.2042-3306.1988.tb01451.xgoogle scholar: lookup
  13. Clark SR, Ma AC, Tavener SA, McDonald B, Goodarzi Z, Kelly MM, Patel KD, Chakrabarti S, McAvoy E, Sinclair GD, Keys EM, Allen-Vercoe E, Devinney R, Doig CJ, Green FH, Kubes P. Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood.. Nat Med 2007 Apr;13(4):463-9.
    pubmed: 17384648doi: 10.1038/nm1565google scholar: lookup
  14. Daniel AJ, Leise BS, Burgess BA, Morley PS, Cloninger M, Hassel DM. Concentrations of serum amyloid A and plasma fibrinogen in horses undergoing emergency abdominal surgery.. J Vet Emerg Crit Care (San Antonio) 2016 May;26(3):344-51.
    pubmed: 26274017doi: 10.1111/vec.12365google scholar: lookup
  15. Davies GR, Mills GM, Lawrence M, Battle C, Morris K, Hawkins K, Williams PR, Davidson S, Thomas D, Evans PA. The role of whole blood impedance aggregometry and its utilisation in the diagnosis and prognosis of patients with systemic inflammatory response syndrome and sepsis in acute critical illness.. PLoS One 2014;9(9):e108589.
    pmc: PMC4182491pubmed: 25269018doi: 10.1371/journal.pone.0108589google scholar: lookup
  16. Elzey BD, Grant JF, Sinn HW, Nieswandt B, Waldschmidt TJ, Ratliff TL. Cooperation between platelet-derived CD154 and CD4+ T cells for enhanced germinal center formation.. J Leukoc Biol 2005 Jul;78(1):80-4.
    pubmed: 15899982doi: 10.1189/jlb.1104669google scholar: lookup
  17. Engel J. Impedanzaggregometrisch bestimmte Thrombozytenfunktion bei klinisch unauffälligen Ponys [Determination of platelet function by impedance aggregometry in healthy ponies]. Abstract presented at: 25. Jahrestagung der FG “Innere Medizin und klinische Labordiagnostik” der DVG (InnLab), 03./04; February 2017; Göttingen, Germany.
  18. Epstein KL, Brainard BM, Gomez-Ibanez SE, Lopes MA, Barton MH, Moore JN. Thrombelastography in horses with acute gastrointestinal disease.. J Vet Intern Med 2011 Mar-Apr;25(2):307-14.
    pubmed: 21314719doi: 10.1111/j.1939-1676.2010.0673.xgoogle scholar: lookup
  19. Gader AG, Ghumlas AK, Hussain MF, Haidari AA, White JG. The ultrastructure of camel blood platelets: a comparative study with human, bovine, and equine cells.. Platelets 2008 Feb;19(1):51-8.
    pubmed: 18231938doi: 10.1080/09537100701627151google scholar: lookup
  20. Grove EL, Hvas AM, Mortensen SB, Larsen SB, Kristensen SD. Effect of platelet turnover on whole blood platelet aggregation in patients with coronary artery disease.. J Thromb Haemost 2011 Jan;9(1):185-91.
    pubmed: 20955349doi: 10.1111/j.1538-7836.2010.04115.xgoogle scholar: lookup
  21. Held LV. Ermittlung von Referenzintervallen der Thrombozytenfunktion mittels Multiplate® Analyzer beim klinisch unauffälligen Warmblutpferd [Determination of reference intervals for platelet function on the Multiplate Analyzer in healthy Warmblood horses]. Dissertation. Justus Liebig University, 2018.
  22. Hooijberg EH, van den Hoven R, Tichy A, Schwendenwein I. Diagnostic and predictive capability of routine laboratory tests for the diagnosis and staging of equine inflammatory disease.. J Vet Intern Med 2014 Sep-Oct;28(5):1587-93.
    pmc: PMC4895560pubmed: 25056342doi: 10.1111/jvim.12404google scholar: lookup
  23. Hübers E, Bauer N, Fey K, Moritz A, Roscher K. [Thrombocytopenia in horses].. Tierarztl Prax Ausg G Grosstiere Nutztiere 2018 Apr;46(2):73-79.
    pubmed: 29727871doi: 10.15653/tpg-170429google scholar: lookup
  24. Jenne CN, Wong CH, Zemp FJ, McDonald B, Rahman MM, Forsyth PA, McFadden G, Kubes P. Neutrophils recruited to sites of infection protect from virus challenge by releasing neutrophil extracellular traps.. Cell Host Microbe 2013 Feb 13;13(2):169-80.
    pubmed: 23414757doi: 10.1016/j.chom.2013.01.005google scholar: lookup
  25. Kim HK, Kim JE, Ham CK, Lee DS, Park S, Cho HI. Prognostic value of platelet indices as determined by ADVIA 120 in patients suspected of having disseminated intravascular coagulation.. Int J Lab Hematol 2008 Apr;30(2):117-23.
    pubmed: 18333843doi: 10.1111/j.1751-553x.2007.00904.xgoogle scholar: lookup
  26. Lambert JL, Fernandez NJ, Roy MF. Association of Presence of Band Cells and Toxic Neutrophils with Systemic Inflammatory Response Syndrome and Outcome in Horses with Acute Disease.. J Vet Intern Med 2016 Jul;30(4):1284-92.
    pmc: PMC5089578pubmed: 27214343doi: 10.1111/jvim.13968google scholar: lookup
  27. Levi M. Platelets at a crossroad of pathogenic pathways in sepsis.. J Thromb Haemost 2004 Dec;2(12):2094-5.
    pubmed: 15613011doi: 10.1111/j.1538-7836.2004.01004.xgoogle scholar: lookup
  28. Macey MG, Carty E, Webb L, Chapman ES, Zelmanovic D, Okrongly D, Rampton DS, Newland AC. Use of mean platelet component to measure platelet activation on the ADVIA 120 haematology system.. Cytometry 1999 Oct 15;38(5):250-5.
    pubmed: 10516612doi: 10.1002/(sici)1097-0320(19991015)38:5<250::aid-cyto8>3.3.co;2-bgoogle scholar: lookup
  29. McDonald B, Urrutia R, Yipp BG, Jenne CN, Kubes P. Intravascular neutrophil extracellular traps capture bacteria from the bloodstream during sepsis.. Cell Host Microbe 2012 Sep 13;12(3):324-33.
    pubmed: 22980329doi: 10.1016/j.chom.2012.06.011google scholar: lookup
  30. McMorran BJ, Marshall VM, de Graaf C, Drysdale KE, Shabbar M, Smyth GK, Corbin JE, Alexander WS, Foote SJ. Platelets kill intraerythrocytic malarial parasites and mediate survival to infection.. Science 2009 Feb 6;323(5915):797-800.
    pubmed: 19197068doi: 10.1126/science.1166296google scholar: lookup
  31. Montrucchio G, Bosco O, Del Sorbo L, Fascio Pecetto P, Lupia E, Goffi A, Omedè P, Emanuelli G, Camussi G. Mechanisms of the priming effect of low doses of lipopoly-saccharides on leukocyte-dependent platelet aggregation in whole blood.. Thromb Haemost 2003 Nov;90(5):872-81.
    pubmed: 14597983doi: 10.1160/th03-02-0085google scholar: lookup
  32. Moreau D, Timsit JF, Vesin A, Garrouste-Orgeas M, de Lassence A, Zahar JR, Adrie C, Vincent F, Cohen Y, Schlemmer B, Azoulay E. Platelet count decline: an early prognostic marker in critically ill patients with prolonged ICU stays.. Chest 2007 Jun;131(6):1735-41.
    pubmed: 17475637doi: 10.1378/chest.06-2233google scholar: lookup
  33. Moritz A. Der Einsatz lasergestützter Multiparameter-Hämatologiesysteme in der Veterinärmedizin [The use of automated laser-based multiparameter hematology systems in veterinary medicine]. Habilitation. Justus Liebig University Giessen, 2000.
  34. Moritz A, Walcheck BK, Weiss DJ. Evaluation of flow cytometric and automated methods for detection of activated platelets in dogs with inflammatory disease.. Am J Vet Res 2005 Feb;66(2):325-9.
    pubmed: 15757135doi: 10.2460/ajvr.2005.66.325google scholar: lookup
  35. Pedersen SB, Grove EL, Nielsen HL, Mortensen J, Kristensen SD, Hvas AM. Evaluation of aspirin response by Multiplate whole blood aggregometry and light transmission aggregometry.. Platelets 2009 Sep;20(6):415-20.
    pubmed: 19658002doi: 10.1080/09537100903100643google scholar: lookup
  36. Peerschke EI, Reid KB, Ghebrehiwet B. Platelet activation by C1q results in the induction of alpha IIb/beta 3 integrins (GPIIb-IIIa) and the expression of P-selectin and procoagulant activity.. J Exp Med 1993 Aug 1;178(2):579-87.
    pmc: PMC2191135pubmed: 7688027doi: 10.1084/jem.178.2.579google scholar: lookup
  37. Prins M, van Leeuwen MW, Teske E. Stability and reproducibility of ADVIA 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CH(R)) in the diagnosis of iron deficiency.. Tijdschr Diergeneeskd 2009 Apr 1;134(7):272-8.
    pubmed: 19431960
  38. Reinhart K, Brunkhorst FM, Bone HG, Bardutzky J, Dempfle CE, Forst H, Gastmeier P, Gerlach H, Gründling M, John S, Kern W, Kreymann G, Krüger W, Kujath P, Marggraf G, Martin J, Mayer K, Meier-Hellmann A, Oppert M, Putensen C, Quintel M, Ragaller M, Rossaint R, Seifert H, Spies C, Stüber F, Weiler N, Weimann A, Werdan K, Welte T. [Prevention, diagnosis, treatment, and follow-up care of sepsis. First revision of the S2k Guidelines of the German Sepsis Society (DSG) and the German Interdisciplinary Association for Intensive and Emergency Care Medicine (DIVI)].. Anaesthesist 2010 Apr;59(4):347-70.
    pubmed: 20414762doi: 10.1007/s00101-010-1719-5google scholar: lookup
  39. Roscher K. Thrombozyten der Equiden - ein unterschätzter Biomarker? [Platelets in equids—an underrated biomarker?]. Habilitation. Justus Liebig University Giessen, 2018.
  40. Roscher KA, Failing K, Moritz A. Inhibition of platelet function with clopidogrel, as measured with a novel whole blood impedance aggregometer in horses.. Vet J 2015 Mar;203(3):332-6.
    pubmed: 25736876doi: 10.1016/j.tvjl.2014.12.028google scholar: lookup
  41. Roy MF, Kwong GP, Lambert J, Massie S, Lockhart S. Prognostic Value and Development of a Scoring System in Horses With Systemic Inflammatory Response Syndrome.. J Vet Intern Med 2017 Mar;31(2):582-592.
    pmc: PMC5354005pubmed: 28207163doi: 10.1111/jvim.14670google scholar: lookup
  42. Russwurm S, Vickers J, Meier-Hellmann A, Spangenberg P, Bredle D, Reinhart K, Lösche W. Platelet and leukocyte activation correlate with the severity of septic organ dysfunction.. Shock 2002 Apr;17(4):263-8.
    pubmed: 11954824doi: 10.1097/00024382-200204000-00004google scholar: lookup
  43. Schwarz BC, van den Hoven R, Schwendenwein I. Diagnostic value of the neutrophil myeloperoxidase index in horses with systemic inflammation.. Vet J 2012 Jan;191(1):72-8.
    pubmed: 21262582doi: 10.1016/j.tvjl.2010.12.010google scholar: lookup
  44. Segura D, Monreal L, Pérez-Pujol S, Pino M, Ordinas A, Brugués R, White JG, Escolar G. Assessment of platelet function in horses: ultrastructure, flow cytometry, and perfusion techniques.. J Vet Intern Med 2006 May-Jun;20(3):581-8.
    pubmed: 16734093doi: 10.1892/0891-6640(2006)20[581:aopfih]2.0.co;2google scholar: lookup
  45. Segura D, Monreal L, Armengou L, Tarancón I, Brugués R, Escolar G. Mean platelet component as an indicator of platelet activation in foals and adult horses.. J Vet Intern Med 2007 Sep-Oct;21(5):1076-82.
    pubmed: 17939567doi: 10.1892/0891-6640(2007)21[1076:mpcaai]2.0.co;2google scholar: lookup
  46. Smyth SS, McEver RP, Weyrich AS, Morrell CN, Hoffman MR, Arepally GM, French PA, Dauerman HL, Becker RC. Platelet functions beyond hemostasis.. J Thromb Haemost 2009 Nov;7(11):1759-66.
    pubmed: 19691483doi: 10.1111/j.1538-7836.2009.03586.xgoogle scholar: lookup
  47. Steverink PJ, Salden HJ, Sturk A, Klein WR, van der Velden MA, Németh F. Laboratory and clinical evaluation of a chromogenic endotoxin assay for horses with acute intestinal disorders.. Vet Q 1994 May;16 Suppl 2:S117-21.
    pubmed: 7801503
  48. Tóth O, Calatzis A, Penz S, Losonczy H, Siess W. Multiple electrode aggregometry: a new device to measure platelet aggregation in whole blood.. Thromb Haemost 2006 Dec;96(6):781-8.
    pubmed: 17139373
  49. Tridente A, Clarke GM, Walden A, Gordon AC, Hutton P, Chiche JD, Holloway PA, Mills GH, Bion J, Stüber F, Garrard C, Hinds C. Association between trends in clinical variables and outcome in intensive care patients with faecal peritonitis: analysis of the GenOSept cohort.. Crit Care 2015 May 5;19(1):210.
    pmc: PMC4432819pubmed: 25939380doi: 10.1186/s13054-015-0931-8google scholar: lookup
  50. van Deventer SJ, Buller HR, ten Cate JW, Sturk A, Pauw W. Endotoxaemia: an early predictor of septicaemia in febrile patients.. Lancet 1988 Mar 19;1(8586):605-9.
    pubmed: 2894546doi: 10.1016/s0140-6736(88)91412-2google scholar: lookup
  51. Verschoor A, Neuenhahn M, Navarini AA, Graef P, Plaumann A, Seidlmeier A, Nieswandt B, Massberg S, Zinkernagel RM, Hengartner H, Busch DH. A platelet-mediated system for shuttling blood-borne bacteria to CD8α+ dendritic cells depends on glycoprotein GPIb and complement C3.. Nat Immunol 2011 Oct 30;12(12):1194-201.
    pubmed: 22037602doi: 10.1038/ni.2140google scholar: lookup
  52. Whitworth NH, Barradas MA, Mikhailidis DP, Dandona P. An investigation into the effects of bacterial lipopolysaccharide on human platelets.. Eur J Haematol 1989 Aug;43(2):112-9.
    pubmed: 2792318doi: 10.1111/j.1600-0609.1989.tb00266.xgoogle scholar: lookup
  53. Zelmanovic D. Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples. United States patent US 5817519 A. 1998 Oct 06.

Citations

This article has been cited 2 times.
  1. Xiao G, Zhang Z, Chen Q, Wu T, Shi W, Gan L, Liu X, Huang Y, Lv M, Zhao Y, Wu P, Zhong L, He J. Platelets for cancer treatment and drug delivery.. Clin Transl Oncol 2022 Jul;24(7):1231-1237.
    doi: 10.1007/s12094-021-02771-xpubmed: 35218523google scholar: lookup
  2. Theuerkauf K, Obach-Schröck C, Staszyk C, Moritz A, Roscher KA. Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.. J Vet Diagn Invest 2022 May;34(3):448-457.
    doi: 10.1177/10406387221077969pubmed: 35168432google scholar: lookup
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