FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2019; 31(3); 448-452; doi: 10.1177/1040638719832340

Assessment of the CoaguChek-XS portable prothrombin time point-of-care analyzer for horses.

Abstract: Coagulopathies in horses are common and potentially life-threatening. In equine field medicine, a portable point-of-care (POC) prothrombin time (PT) testing device could be useful to identify early changes in extrinsic clotting. The CoaguChek-XS (Roche Diagnostics) is a small, portable POC PT analyzer used in human medicine. Our preliminary study assessed the suitability of CoaguChek-XS for testing PT in horses and established the PT reference interval (PT RI) in healthy horses using this instrument. Blood samples collected from 102 healthy and ill horses were analyzed with the CoaguChek-XS and compared to a semi-automated coagulometric analyzer (SACA) as the gold standard. There was a significant positive correlation between the 2 measurement methods ( r = 0.765, p < 0.01), and very good agreement, with 97% of the samples falling within limits of agreement. The mean CoaguChek-XS PT coefficient of variation was 0.8%, indicating high precision. With high precision and good agreement with the coagulometric PT, the CoaguChek-XS should be further validated for PT measurement in horses.
Get Full Text
Publication Date: 2019-03-11 PubMed ID: 30852982PubMed Central: PMC6838704DOI: 10.1177/1040638719832340Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article examines the effectiveness of the CoaguChek-XS, a point-of-care testing device, in assessing the prothrombin time (PT) in horses, which may aid in early detection of clotting disorders. The study shows a significant correlation with the standard method of measurement and suggests the CoaguChek-XS might be a valuable tool in horse health management.

About the Research

  • The study revolves around CoaguChek-XS, a portable device commonly used in human medicine to measure prothrombin time or PT, a test that measures how quickly the blood clots.
  • The researchers wanted to see if this device could also work effectively for horses, as blood clotting problems can be common and serious in equines.

Methodology

  • The scientists tested the CoaguChek-XS on blood samples drawn from 102 horses, both healthy and ill.
  • They then compared the results from the CoaguCheck-XS to those obtained using a semi-automated coagulometric analyzer (SACA), considered the “gold standard” for such tests.

Results and Interpretation

  • Analysis showed a significant positive correlation between the measurements obtained from the two methods. This means that as the PT values increased in the SACA measurements, they also increased in the CoaguChek-XS measurements, and vice versa.
  • In fact, 97% of the samples’ results aligned with each other regarding the limits of agreement, suggesting that the CoaguChek-XS can yield results highly consistent with those from the SACA.
  • The study also found that the CoaguChek-XS shows high precision (as indicated by the coefficient of variation being only 0.8%). This means the CoaguChek-XS provides very consistent measurements for the same samples.

Conclusions and Future Directions

  • The research concludes that the portable CoaguChek-XS device could be a useful tool in equine medicine for assessing PT in the field.
  • Given its demonstrated high precision and agreement with the gold standard coagulometric PT measurement, the researchers recommend that the CoaguChek-XS be further validated for PT measurement in horses.

Cite This Article

APA
Berlin N, Kelmer E, Segev G, Aroch I, Kelmer G. (2019). Assessment of the CoaguChek-XS portable prothrombin time point-of-care analyzer for horses. J Vet Diagn Invest, 31(3), 448-452. https://doi.org/10.1177/1040638719832340

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 31
Issue: 3
Pages: 448-452

Researcher Affiliations

Berlin, Noa
  • Departments of Small Animal Emergency and Critical Care (Berlin, E Kelmer).
  • Small Animal Internal Medicine (Segev, Aroch).
  • Large Animal Medicine and Surgery (G Kelmer).
  • The Hebrew University Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Kelmer, Efrat
  • Departments of Small Animal Emergency and Critical Care (Berlin, E Kelmer).
  • Small Animal Internal Medicine (Segev, Aroch).
  • Large Animal Medicine and Surgery (G Kelmer).
  • The Hebrew University Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Segev, Gilad
  • Departments of Small Animal Emergency and Critical Care (Berlin, E Kelmer).
  • Small Animal Internal Medicine (Segev, Aroch).
  • Large Animal Medicine and Surgery (G Kelmer).
  • The Hebrew University Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Aroch, Itamar
  • Departments of Small Animal Emergency and Critical Care (Berlin, E Kelmer).
  • Small Animal Internal Medicine (Segev, Aroch).
  • Large Animal Medicine and Surgery (G Kelmer).
  • The Hebrew University Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Kelmer, Gal
  • Departments of Small Animal Emergency and Critical Care (Berlin, E Kelmer).
  • Small Animal Internal Medicine (Segev, Aroch).
  • Large Animal Medicine and Surgery (G Kelmer).
  • The Hebrew University Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.

MeSH Terms

  • Animals
  • Disseminated Intravascular Coagulation / diagnosis
  • Disseminated Intravascular Coagulation / veterinary
  • Female
  • Horse Diseases / diagnosis
  • Horses
  • Male
  • Point-of-Care Systems
  • Prothrombin Time / methods
  • Prothrombin Time / veterinary
  • Sensitivity and Specificity

Conflict of Interest Statement

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

References

This article includes 22 references
  1. Bereznicki LR, Jackson SL, Peterson GM, Jeffrey EC, Marsden KA, Jupe DM. Accuracy and clinical utility of the CoaguChek XS portable international normalised ratio monitor in a pilot study of warfarin home-monitoring.. J Clin Pathol 2007 Mar;60(3):311-4.
    pmc: PMC1860570pubmed: 16751303doi: 10.1136/jcp.2006.037820google scholar: lookup
  2. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement.. Lancet 1986 Feb 8;1(8476):307-10.
    pubmed: 2868172
  3. Dallap BL. Coagulopathy in the equine critical care patient.. Vet Clin North Am Equine Pract 2004 Apr;20(1):231-51.
    pubmed: 15062467doi: 10.1016/j.cveq.2003.11.002google scholar: lookup
  4. Dallap Schaer BL, Epstein K. Coagulopathy of the critically ill equine patient.. J Vet Emerg Crit Care (San Antonio) 2009 Feb;19(1):53-65.
    pubmed: 19691585doi: 10.1111/j.1476-4431.2009.00390.xgoogle scholar: lookup
  5. Flatland B, Freeman KP, Vap LM, Harr KE. ASVCP guidelines: quality assurance for point-of-care testing in veterinary medicine.. Vet Clin Pathol 2013 Dec;42(4):405-23.
    pubmed: 24320778doi: 10.1111/vcp.12099google scholar: lookup
  6. Friedrichs KR, Harr KE, Freeman KP, Szladovits B, Walton RM, Barnhart KF, Blanco-Chavez J. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics.. Vet Clin Pathol 2012 Dec;41(4):441-53.
    pubmed: 23240820doi: 10.1111/vcp.12006google scholar: lookup
  7. Harr KE, Flatland B, Nabity M, Freeman KP. ASVCP guidelines: allowable total error guidelines for biochemistry.. Vet Clin Pathol 2013 Dec;42(4):424-36.
    pubmed: 24320779doi: 10.1111/vcp.12101google scholar: lookup
  8. Jensen AL, Kjelgaard-Hansen M. Method comparison in the clinical laboratory.. Vet Clin Pathol 2006 Sep;35(3):276-86.
    pubmed: 16967409doi: 10.1111/j.1939-165x.2006.tb00131.xgoogle scholar: lookup
  9. Kapiotis S, Quehenberger P, Speiser W. Evaluation of the new method Coaguchek for the determination of prothrombin time from capillary blood: comparison with Thrombotest on KC-1.. Thromb Res 1995 Mar 15;77(6):563-7.
    pubmed: 7624842doi: 10.1016/0049-3848(95)00031-3google scholar: lookup
  10. Kelmer E, Segev G, Codner C, Bruchim Y, Klainbart S, Aroch I. Assessment of a portable prothrombin time analyzer (CoaguChek-XS) in dogs.. J Vet Emerg Crit Care (San Antonio) 2014 Jul-Aug;24(4):455-60.
    pubmed: 25039332doi: 10.1111/vec.12200google scholar: lookup
  11. Levi M, Ten Cate H. Disseminated intravascular coagulation.. N Engl J Med 1999 Aug 19;341(8):586-92.
    pubmed: 10451465doi: 10.1056/nejm199908193410807google scholar: lookup
  12. Monreal L, Cesarini C. Coagulopathies in horses with colic.. Vet Clin North Am Equine Pract 2009 Aug;25(2):247-58.
    pubmed: 19580937doi: 10.1016/j.cveq.2009.04.001google scholar: lookup
  13. Morris DD. Endotoxemia in horses. A review of cellular and humoral mediators involved in its pathogenesis.. J Vet Intern Med 1991 May-Jun;5(3):167-81.
    pubmed: 1920254doi: 10.1111/j.1939-1676.1991.tb00944.xgoogle scholar: lookup
  14. Nabity MB, Harr KE, Camus MS, Flatland B, Vap LM. ASVCP guidelines: Allowable total error hematology.. Vet Clin Pathol 2018 Mar;47(1):9-21.
    pubmed: 29430668doi: 10.1111/vcp.12583google scholar: lookup
  15. Newbould AR, Norman EJ. Comparison of point-of-care analysis using Coaguchek XS and standard laboratory-measured prothrombin time in dogs.. N Z Vet J 2013 Jan;61(1):18-24.
    pubmed: 22984842doi: 10.1080/00480169.2012.709816google scholar: lookup
  16. Peek SF, Semrad SD, Perkins GA. Clostridial myonecrosis in horses (37 cases 1985-2000).. Equine Vet J 2003 Jan;35(1):86-92.
    pubmed: 12553469doi: 10.2746/042516403775467513google scholar: lookup
  17. Plesch W, Wolf T, Breitenbeck N, Dikkeschei LD, Cervero A, Perez PL, van den Besselaar AM. Results of the performance verification of the CoaguChek XS system.. Thromb Res 2008;123(2):381-9.
    pubmed: 18585761doi: 10.1016/j.thromres.2008.04.021google scholar: lookup
  18. Poller L, Keown M, Ibrahim SA, van der Meer FJ, van den Besselaar AM, Tripodi A, Jespersen J, Meijer P, Kluft C. Quality assessment of CoaguChek point-of-care prothrombin time monitors: comparison of the European community-approved procedure and conventional external quality assessment.. Clin Chem 2006 Oct;52(10):1843-7.
    pubmed: 16998117doi: 10.1373/clinchem.2006.071639google scholar: lookup
  19. Valentine BA. Pathologic findings in equine muscle (excluding polysaccharide storage): a necropsy study.. J Vet Diagn Invest 2008 Sep;20(5):572-9.
    pubmed: 18776088doi: 10.1177/104063870802000506google scholar: lookup
  20. van den Besselaar AM, Meeuwisse-Braun J, Schaefer-van Mansfeld H, van Rijn C, Witteveen E. A comparison between capillary and venous blood international normalized ratio determinations in a portable prothrombin time device.. Blood Coagul Fibrinolysis 2000 Sep;11(6):559-62.
    pubmed: 10997796doi: 10.1097/00001721-200009000-00008google scholar: lookup
  21. Weiss DJ, Wardrop KJ, eds. Schalm’s Veterinary Hematology. 6th ed. Ames, IA: Wiley-Blackwell, 2011:1232.
  22. Werners AH, Bull S, Fink-Gremmels J. Endotoxaemia: a review with implications for the horse.. Equine Vet J 2005 Jul;37(4):371-83.
    pubmed: 16028631doi: 10.2746/0425164054529418google scholar: lookup

Citations

This article has been cited 1 times.
  1. Tonthong S, Rungpupradit J. Coagulation testing: Comparison of portable (CoaguChek(®) XS) and automated coagulation analyzer in healthy cats. Vet World 2020 Nov;13(11):2541-2545.
    doi: 10.14202/vetworld.2020.2541-2545pubmed: 33363352google scholar: lookup
Subscribe to our newsletter
Join 100,127 horse owners like you who receive our email updates on horse health and nutrition.