Assessment of the rate of solid-phase gastric emptying in ponies by means of the 13C-octanoic acid breath test: a preliminary study.
Abstract: The aim of this study was to assess the feasibility of applying the 13C-octanoic acid breath test for assessment of gastric emptying in ponies by investigating the pattern of 13C enrichment in breath following the administration of a test meal +/- 13C-octanoic acid. After a 14 h fast, the ponies received either no meal (Test I) or a standardised test meal labelled with 0 mg (Test II), 125 mg (Test III), 250 mg (Test IV) or 500 mg (Test V) 13C-octanoic acid. For each test (I-V), exhaled breath samples were collected in duplicate at 1 h and immediately before ingestion of the test meal and at frequent intervals thereafter for 12 h. Breath samples were analysed by continuous flow isotope ratio mass spectrometry. Three indices of breath 13C-enrichment were computed; half dose recovery time (t1/2), gastric emptying coefficient (GEC) and time to peak breath 13C-enrichment t(max). For Tests I and II, the ratio of 13CO2:12CO2 remained stable for the duration of the sampling period. For Tests III, IV and V, an increase in the ratio of 13CO2:12CO2 was detected. The test was reproducible within individuals, and intersubject variation was low. Further validation studies of this noninvasive technique are justified.
Publication Date: 2001-03-27 PubMed ID: 11266071DOI: 10.1111/j.2042-3306.2001.tb00601.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Validation Study
Summary
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This research aims to establish the validity of using the 13C-octanoic acid breath test to measure the rate of gastric emptying in ponies. The study tested the procedure on ponies in different scenarios, and the results suggest a reliable and consistent breath test conducive to further studies.
Research Procedure and Approach
- The research aimed to check the feasibility of using the 13C-octanoic acid breath test, a noninvasive method, for assessing gastric emptying in ponies. Gastric emptying refers to the speed at which food passes from the stomach to the small intestine.
- They monitored the pattern of 13C enrichment in the ponies’ breath after giving them a test meal with varying amounts of 13C-octanoic acid. The ponies were first fasted for 14 hours before the study.
- Different tests were performed with different dosages of 13C-octanoic acid, including no meal (Test I) and meal labeled with 0 mg (Test II), 125 mg (Test III), 250 mg (Test IV), or 500 mg (Test V).
- After each test, breath samples of the ponies were collected in duplicate at the first hour, before ingesting the meal, and then at regular intervals for 12 hours post-meal.
- These samples were then analyzed via continuous flow isotope ratio mass spectrometry to detect the 13C isotopes in the breath samples.
Outcome and Findings
- The indices computed from this research were half dose recovery time (t1/2), gastric emptying coefficient (GEC), and time to peak breath 13C-enrichment (tmax).
- For the first two tests, where no 13C-octanoic acid was ingested, the ratio of 13CO2:12CO2 in the breath samples remained stable throughout the sampling period. This indicates that without 13C-octanoic acid, the isotope ratio remained unaltered.
- However, in Tests III, IV, and V, when ponies were fed with 13C-octanoic acid, an increase in the ratio of 13CO2:12CO2 was observed, indicating the metabolic process was effectively incorporating the consumed 13C-octanoic acid.
- The study concluded that the test was reproducible within individuals, and there was low variation between different subjects.
- Given the successful preliminary outcome of this study, the researchers recommend further validation of this noninvasive technique for assessing gastric emptying in animals.
Cite This Article
APA
Wyse CA, Murphy DM, Preston T, Morrison DJ, Love S.
(2001).
Assessment of the rate of solid-phase gastric emptying in ponies by means of the 13C-octanoic acid breath test: a preliminary study.
Equine Vet J, 33(2), 197-203.
https://doi.org/10.1111/j.2042-3306.2001.tb00601.x Publication
Researcher Affiliations
- Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden, UK.
MeSH Terms
- Animal Feed
- Animals
- Breath Tests
- Caprylates / metabolism
- Carbon Isotopes
- Gastric Emptying / physiology
- Horses / physiology
- Predictive Value of Tests
- Reference Values
- Time Factors
Citations
This article has been cited 2 times.- Camilleri M, Linden DR. Measurement of Gastrointestinal and Colonic Motor Functions in Humans and Animals. Cell Mol Gastroenterol Hepatol 2016 Jul;2(4):412-428.
- Sasaki N, Tsuzuki N, Yamada M, Minami T, Yamada H. A preliminary study of (13)c-phenylalanine and (13)c-dipeptide breath tests in horses. J Equine Sci 2009;20(1):7-10.
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