Association between single nucleotide polymorphisms in p53 and abortion in Thoroughbred mares.
Abstract: Single nucleotide polymorphisms (SNPs) in the p53 gene have been studied extensively in humans. The aims of this study were to determine the frequency of the Arg/Pro SNP in p53 in Thoroughbred mares on one stud in Brazil and to correlate p53 genotypes with reproductive performance. SNPs were detected by PCR-restriction fragment length polymorphism in blood samples from 105 horses and confirmed by sequencing. The allele frequency in Thoroughbred mares at codon 72 in exon 4 was 73.3% Arg/Pro, 17.1% Arg/Arg and 9.6% Pro/Pro. The presence of Arg/Pro was significantly associated with abortion (P=0.02), while Pro/Pro mares had a lower probability of abortion (P<0.05). Using a logistic regression model, the dominance effect was significant (P=0.044; odds ratio 7.94) for abortion and additive effects were not significant (P=0.26). p53 may play a role in equine reproduction.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication Date: 2012-03-11 PubMed ID: 22414890DOI: 10.1016/j.tvjl.2012.02.003Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research aimed to investigate the correlation between variations in the p53 gene and instances of abortion in Thoroughbred mares. It found that horses carrying certain genetic variations were significantly more prone to abortion, suggesting a vital role of the p53 gene in equine reproduction.
Objective and Methods
- The study sought to investigate the frequency of a specific genetic variation, Arg/Pro SNP, in the p53 gene among Thoroughbred mares at a stud in Brazil, and establish a correlation between p53 genotypes and reproductive performance, particularly concerning instances of abortions.
- The researchers employed PCR-restriction fragment length polymorphism, a method used for the detection of genetic variations, to study blood samples collected from 105 horses. The results were corroborated by sequencing.
Findings
- The frequency of the Arg/Pro variant at codon 72 in exon 4 was found to be the highest (73.3%) among the samples whereas the Pro/Pro variant was detected in 9.6% samples, and the Arg/Arg variant in 17.1% samples.
- A significant association was observed between the presence of the Arg/Pro variant and a higher incidence of abortion (P=0.02); mares carrying the Pro/Pro variant had a lower probability of abortion (P<0.05).
Statistical Analysis
- The researchers employed a logistic regression model to estimate the effects of the genetic variants.
- The dominance effect, which refers to one variant’s predominant influence over another, was found to be significant (P=0.044) for abortions. This was calculated with an odds ratio of 7.94, indicating that horses carrying the Arg/Pro variant are almost eight times more likely to undergo an abortion than horses with different variants.
- No significant additive effect was observed (P=0.26), i.e., having an additional problematic gene or variant did not significantly increase the possibility of an abortion.
Conclusion
- Based on the significantly higher incidence of abortions among the mares carrying the Arg/Pro variant in the p53 gene, the study concludes that the p53 gene could play a pivotal role in equine reproduction.
Cite This Article
APA
Leon PM, Campos VF, Thurow HS, Hartwig FP, Selau LP, Dellagostin OA, Neto JB, Deschamps JC, Seixas FK, Collares T.
(2012).
Association between single nucleotide polymorphisms in p53 and abortion in Thoroughbred mares.
Vet J, 193(2), 573-575.
https://doi.org/10.1016/j.tvjl.2012.02.003 Publication
Researcher Affiliations
- Laboratório de Embriologia Molecular e Transgênese, Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
MeSH Terms
- Abortion, Veterinary / epidemiology
- Abortion, Veterinary / etiology
- Abortion, Veterinary / genetics
- Animals
- Arginine / genetics
- Brazil / epidemiology
- Codon
- Exons
- Female
- Gene Frequency
- Genes, p53
- Genetic Association Studies / veterinary
- Genotype
- Horse Diseases / epidemiology
- Horse Diseases / etiology
- Horse Diseases / genetics
- Horses
- Logistic Models
- Polymerase Chain Reaction / veterinary
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide
- Pregnancy
- Proline / genetics
- Sequence Analysis, DNA / veterinary
Citations
This article has been cited 2 times.- Roach J, Arango Sabogal JC, Smith K, Foote A, Verheyen K, de Mestre AM. Multivariable analysis to determine risk factors associated with abortion in mares. Reprod Fertil 2022 Nov 1;3(4):301-12.
- Laseca N, Anaya G, Peña Z, Pirosanto Y, Molina A, Demyda Peyrás S. Impaired Reproductive Function in Equines: From Genetics to Genomics. Animals (Basel) 2021 Feb 3;11(2).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
