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Home / Equine Research Bank / Immunology / Bacteriostatic effects of horse sera and serum fractions on ...
Immunology1967; 12(3); 285-301;

Bacteriostatic effects of horse sera and serum fractions on Clostridium welchii Type A, and the abolition of bacteriostasis by iron salts.

Abstract: Under a variety of conditions of concentration, Eh, and pH, horse anti- serum and normal horse serum exerted similar bacteriostatic effects against Type A. Ferric iron abolished the bacteriostatic effect when added during the first 2 hours of incubation at Eh+60 mV. Ferrous iron abolished the bacteriostatic effect when added after 3 hours. Ferric iron abolished the bacteriostatic effect at—140 mV. A mixture consisting of horse β- and γ-globulins together with human transferrin exerted a bacteriostatic effect similar to that of whole serum. This system responded in the same way as whole serum to the addition of iron. A mixture of horse β- and γ-globulins exerted an immediate bactericidal effect which could not be prevented by ferric iron.
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Publication Date: 1967-03-01 PubMed ID: 4164032PubMed Central: PMC1409119
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigated the effects of horse serum and its fractions on the bacteria Clostridium welchii Type A, exploring how iron salts can negate these effects.

Research Context

  • The research was aimed at scrutinising the bacteriostatic effects of horse serum and its fractions on Clostridium welchii Type A, a kind of bacteria.
  • The researchers also studied the role of iron salts, particularly ferrous and ferric iron, in negating these bacteriostatic effects.

Research Findings

  • Under various conditions such as concentration, Eh (a measure of the oxidizing or reducing potential of a substance), and pH levels, both horse anti-serum and normal horse serum displayed similar bacteriostatic effects against Clostridium welchii Type A.
  • Introducing ferric iron during the first two hours of incubation at an Eh+60 mV setting effectively abolished this bacteriostatic effect. Similarly, introducing ferrous iron after 3 hours had the same result.
  • Also, the application of ferric iron eradicated the bacteriostatic effect at negative 140 mV.

Role of Serum Components

  • A system comprising of horse beta- and gamma-globulins along with human transferrin showed a bacteriostatic effect similar to that of the whole serum.
  • Interestingly, this system, like whole serum, lost its bacteriostatic potency when iron was introduced into it.
  • On the other hand, a combination of horse beta- and gamma-globulins demonstrated an immediate bactericidal (bacteria-killing) effect, unaffected by the addition of ferric iron.

Significance of the Research

  • This investigation provides valuable insights into the bacteriostatic and bactericidal effects of different components of horse serum and how these can be manipulated through the inclusion of iron.
  • These findings can act as a foundation for future studies on antibiotic therapies and provide greater understanding of how the bacteriostatic and bactericidal properties of substances can be controlled for optimal therapeutic benefits.

Cite This Article

APA
Rogers HJ. (1967). Bacteriostatic effects of horse sera and serum fractions on Clostridium welchii Type A, and the abolition of bacteriostasis by iron salts. Immunology, 12(3), 285-301.

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 12
Issue: 3
Pages: 285-301

Researcher Affiliations

Rogers, H J

    MeSH Terms

    • Animals
    • Beta-Globulins / pharmacology
    • Clostridium perfringens / drug effects
    • Clostridium perfringens / immunology
    • Horses
    • Immune Sera / pharmacology
    • In Vitro Techniques
    • Iron / pharmacology
    • Transferrin / pharmacology
    • gamma-Globulins / pharmacology

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    Citations

    This article has been cited 11 times.
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