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Home / Equine Research Bank / Biomed Res Int / Basic Studies on the Oxidative Stress Markers in Two Types o...
BioMed research international2020; 2020; 7542384; doi: 10.1155/2020/7542384

Basic Studies on the Oxidative Stress Markers in Two Types of Horse Breed: Semi-isolated Population of Huculs Is Different from Commercially Used Arabian Horses.

Abstract: Hucul and Arabian horses differ in the physiological constitution and exposition to environmental conditions. Oxidative stress plays a pathogenic role in many diseases and enables further injuries. The objective of this study was to compare the levels of enzymatic and nonenzymatic oxidative stress markers in Hucul horses living in seminatural conditions and in commercially handled Arabian horses. We tested the serum samples for total superoxide dismutase (total SOD), Cu-Zn-superoxide dismutase (CuZnSOD), and Mn-dependent superoxide dismutase (MnSOD) activity; for lipofuscin (LPS), ceruloplasmin (CER) and malondialdehyde (MDA) concentration; and for total antioxidant capacity (TAC) and total oxidant status (TOS). Total SOD ( < 0.001), MnSOD ( < 0.001), and CuZnSOD ( < 0.001) activities were significantly higher whereas LPS ( < 0.05), TAC ( < 0.001), TOS ( < 0.001), and MDA ( < 0.001) concentrations were significantly lower in the serum samples collected from Huculs vs. Arabian horses, regardless of the gender. Gender, regardless of the breed, had no significant impact on the antioxidants' activity and concentration. Total SOD and MnSOD activities were significantly higher in Hucul's mares when compared to Hucul's stallions. Concentrations of TAC and TOS were significantly lower in Arabian stallions than in Arabian mares. Commercially handled horses expressed a higher level of oxidative stress markers in comparison to breeds living in seminatural conditions. We conclude that antioxidants are important biomarkers of animal health, whether they are under maintenance care or performing physical exercise.
Copyright © 2020 Barbara A. Bażanów et al.
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Publication Date: 2020-07-13 PubMed ID: 32733953PubMed Central: PMC7376404DOI: 10.1155/2020/7542384Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a scientific investigation conducted to compare oxidative stress markers in two different horse breeds namely, Hucul horses, living semi-natural conditions, and Arabian horses, subjected to commercial use.

Study Objective

  • The primary aim of the study was to analyze the levels of enzymatic and non-enzymatic oxidative stress markers in two different horses breeds, to look at the effect of their living conditions (semi-natural versus commercial) on their biochemical parameters related to oxidative stress.

Methods

  • The researchers collected serum samples from both horse breeds.
  • They then conducted a number of tests on these serum samples to measure the activity of total superoxide dismutase (total SOD), Cu-Zn-superoxide dismutase (CuZnSOD), and Mn-dependent superoxide dismutase (MnSOD); as well as concentrations of lipofuscin (LPS), ceruloplasmin (CER), and malondialdehyde (MDA).
  • Furthermore, they determined the total antioxidant capacity (TAC) and total oxidant status (TOS) of both horse breeds.

Results

  • Significantly higher levels of total SOD, MnSOD, and CuZnSOD activities were found in Hucul horses as compared to Arabian horses, while the levels of LPS, TAC, TOS, and MDA were significantly lower in Hucul horses.
  • No significant differences were observed in the activity and concentration of antioxidants according to gender, irrespective of the horse breed. However, some gender-dependent differences were noted within the same breed; for example, Hucul mares showed significantly higher activities of total SOD and MnSOD than Hucul stallions. Similarly, TAC and TOS were significantly lower in Arabian stallions than in Arabian mares.
  • In comparison to breeds living in semi-natural conditions, commercially handled horses expressed a higher level of oxidative stress markers.

Conclusion

  • The study concluded that antioxidants (measured through various biomarkers in this study) play a critical role in animal health, be it in horses that are maintained for care or those involved in physical exercise.
  • This study also sheds light on the effect of different environmental conditions on the level of oxidative stress, as measured by various biomarkers, in different horse breeds. Commercially kept horses showed higher oxidative stress levels compared to those living in semi-natural conditions.

Cite This Article

APA
Bażanów BA, Chełmecka E, Romuk E, Stygar DM. (2020). Basic Studies on the Oxidative Stress Markers in Two Types of Horse Breed: Semi-isolated Population of Huculs Is Different from Commercially Used Arabian Horses. Biomed Res Int, 2020, 7542384. https://doi.org/10.1155/2020/7542384

Publication

BioMed research international
ISSN: 2314-6141
NlmUniqueID: 101600173
Country: United States
Language: English
Volume: 2020
Pages: 7542384
PII: 7542384

Researcher Affiliations

Bażanów, Barbara A
  • Division of Microbiology, Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wrocław 50-375, Poland.
Chełmecka, Elżbieta
  • Department of Statistics, Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences in Sosnowiec Medical University of Silesia, 40-055 Katowice, Poland.
Romuk, Ewa
  • Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, Poland.
Stygar, Dominika M
  • Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-751 Katowice, Poland.

MeSH Terms

  • Animals
  • Antioxidants / metabolism
  • Biomarkers / blood
  • Breeding
  • Ceruloplasmin / metabolism
  • Female
  • Horses / blood
  • Male
  • Oxidative Stress

Conflict of Interest Statement

The authors declare that they have no conflict of interest.

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Citations

This article has been cited 3 times.
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  2. Kosaruk W, Brown JL, Towiboon P, Punyapornwithaya V, Pringproa K, Thitaram C. Measures of Oxidative Status Markers in Relation to Age, Sex, and Season in Sick and Healthy Captive Asian Elephants in Thailand.. Animals (Basel) 2023 May 5;13(9).
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  3. Poloczek J, Kazura W, Chełmecka E, Michalczyk K, Jochem J, Gumprecht J, Stygar D. Duodenojejunal Omega Switch Surgery Reduces Oxidative Stress Induced by Cafeteria Diet in Sprague-Dawley Rats.. Nutrients 2022 Oct 2;14(19).
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