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Home / Equine Research Bank / Viruses / BCG Immunotherapy in Equine Sarcoid Treatment: Mechanisms, C...
Viruses2025; 17(10); 1322; doi: 10.3390/v17101322

BCG Immunotherapy in Equine Sarcoid Treatment: Mechanisms, Clinical Efficacy, and Challenges in Veterinary Oncology.

Abstract: Equine sarcoids are the most common dermatological neoplasm in horses worldwide, associated with bovine papillomavirus (BPV) infection and characterized by high recurrence rates after conventional therapies. Bacillus Calmette-Guérin (BCG) immunotherapy has historically been used for sarcoid treatment, yet its role in contemporary veterinary oncology remains debated. This narrative review critically examines the immunological mechanisms, clinical efficacy, and limitations of BCG in equine sarcoid therapy, while integrating insights from comparative oncology and One Health perspectives. A systematic search following PRISMA-based criteria identified 55 relevant studies published over the past four decades. Evidence indicates that BCG activates innate and adaptive immunity through TLR2/4 signaling, macrophage polarization, and enhanced CD8+ T-cell responses, leading to partial or complete sarcoid regression in select cases. However, therapeutic outcomes are highly variable due to heterogeneity in protocols (dose, strain, adjuvant use) and frequent adverse inflammatory reactions. Comparative analyses highlight that modern alternatives-such as cryotherapy, cisplatin-based protocols, and topical imiquimod-achieve higher efficacy and lower recurrence rates in many clinical settings. Although BCG is now rarely considered a first-line therapy, it remains relevant in resource-limited regions, such as the Amazon Biome, where cost-effectiveness and accessibility are critical. Future directions include randomized controlled trials, standardized protocols, and innovative approaches such as checkpoint inhibition, CRISPR-Cas9 targeting of viral oncogenes, and nanoparticle delivery systems. This review provides a balanced and data-driven synthesis of BCG immunotherapy, clarifying its historical contributions, current limitations, and translational opportunities for advancing equine and comparative oncology.
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Publication Date: 2025-09-29 PubMed ID: 41157593PubMed Central: PMC12567874DOI: 10.3390/v17101322Google Scholar: Lookup
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  • Review

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Overview

  • This research article reviews the use of Bacillus Calmette-Guérin (BCG) immunotherapy for treating equine sarcoids, a common skin tumor in horses related to bovine papillomavirus infection.
  • It evaluates the immunological mechanisms behind BCG, clinical effectiveness, challenges, and compares it with other modern treatments, while suggesting future research directions.

Background and Context

  • Equine Sarcoids: The most frequent skin tumor in horses worldwide, often caused by bovine papillomavirus (BPV).
  • Clinical Challenge: Sarcoids have high recurrence rates after conventional treatments such as surgery, cryotherapy, or chemical agents.
  • BCG Immunotherapy: Historically used in veterinary oncology to stimulate the immune system in an attempt to regress or eliminate sarcoids.
  • Relevance: Despite its historical use, BCG’s role today is debated due to variable efficacy and potential side effects.

Methodology

  • The article is a narrative review based on a systematic literature search using PRISMA criteria.
  • A total of 55 studies from the last 40 years were evaluated.
  • Focus was on immunological mechanisms, clinical outcomes, therapy protocols, adverse effects, and comparative oncology perspectives.

Immunological Mechanisms of BCG in Sarcoid Treatment

  • Immune Activation: BCG stimulates both innate and adaptive immune responses.
  • Pattern Recognition: Through Toll-like receptors (TLR2 and TLR4), BCG activates macrophages and dendritic cells.
  • Cell Polarization: Macrophage polarization enhances inflammatory and cytotoxic responses against tumor cells.
  • Adaptive Responses: CD8+ T-cell activity is increased, promoting targeted killing of BPV-infected tumor cells.
  • Result: These mechanisms contribute to partial or complete regression of sarcoids in some cases.

Clinical Efficacy and Variability

  • BCG treatment results are highly variable across different cases.
  • Variability is linked to:
    • Differences in treatment protocols such as BCG dose, strain variation, and use of adjuvants.
    • Inconsistent induction of immune responses between individual horses.
    • Frequent adverse inflammatory reactions that can limit treatment tolerance.
  • Although some horses show sarcoid regression, these outcomes are not reliably reproducible.

Comparative Oncology and Alternative Therapies

  • Modern alternatives often demonstrate better outcomes and fewer side effects, including:
    • Cryotherapy: Freezing tumors with liquid nitrogen.
    • Cisplatin-based Protocols: Chemotherapeutic agents injected locally.
    • Topical Imiquimod: An immune response modifier applied directly to sarcoids.
  • These therapies frequently result in higher efficacy and lower sarcoid recurrence rates compared to BCG.
  • The comparative analysis highlights the limited role of BCG as a first-line therapy in resource-rich clinical settings.

Contextual and Regional Considerations

  • BCG remains relevant in resource-limited regions, such as the Amazon Biome, because:
    • It is cost-effective compared to certain expensive alternatives.
    • It is more accessible where advanced veterinary oncology resources are scarce.
  • This underlines the importance of considering socio-economic and geographic factors in treatment choice.

Challenges and Limitations

  • Protocol heterogeneity impedes consistent clinical application and assessment of efficacy.
  • Adverse inflammatory effects are common and can complicate therapy.
  • Lack of robust randomized controlled trials leaves many questions about optimal usage unanswered.
  • BCG’s immunotherapeutic effect is not universally effective due to tumor and host immune system variability.

Future Directions and Innovations

  • Call for randomized controlled trials to better define BCG’s place in therapy and optimize protocols.
  • Development of standardized treatment protocols (dose, strain, administration method) is needed.
  • Exploration of innovative therapies combining or replacing BCG, such as:
    • Checkpoint inhibitors to enhance immune response against BPV-driven tumors.
    • CRISPR-Cas9 gene-editing technology aimed at disrupting viral oncogenes in sarcoid cells.
    • Nanoparticle-based delivery systems to improve targeting and reduce side effects.
  • Integration of comparative oncology and One Health perspectives to translate findings between human and veterinary medicine.

Summary and Conclusion

  • This review provides a balanced and evidence-based synthesis of BCG immunotherapy for equine sarcoids.
  • It clarifies the immune mechanisms by which BCG works and highlights its historical significance in veterinary oncology.
  • The article identifies the current limitations and variability in clinical outcomes leading to decreased use as a first choice treatment.
  • Offers a forward-looking view emphasizing the potential for innovative immunotherapies to improve equine and comparative oncology treatment paradigms.

Cite This Article

APA
Monteiro MM, de Castro ELA, Pereira AJM, Thiesen R, Thiesen RMC, Salvarani FM. (2025). BCG Immunotherapy in Equine Sarcoid Treatment: Mechanisms, Clinical Efficacy, and Challenges in Veterinary Oncology. Viruses, 17(10), 1322. https://doi.org/10.3390/v17101322

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 17
Issue: 10
PII: 1322

Researcher Affiliations

Monteiro, Mariana Martins
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal 68740-970, PA, Brazil.
de Castro, Elcidimar Lucas Aleixo
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal 68740-970, PA, Brazil.
Pereira, Ana Júlia Moaraes
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal 68740-970, PA, Brazil.
Thiesen, Roberto
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal 68740-970, PA, Brazil.
Thiesen, Roberta Martins Crivelaro
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal 68740-970, PA, Brazil.
Salvarani, Felipe Masiero
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal 68740-970, PA, Brazil.

MeSH Terms

  • Animals
  • Horses
  • Horse Diseases / therapy
  • Horse Diseases / immunology
  • Immunotherapy / veterinary
  • Immunotherapy / methods
  • Skin Neoplasms / veterinary
  • Skin Neoplasms / therapy
  • Skin Neoplasms / immunology
  • BCG Vaccine / therapeutic use
  • BCG Vaccine / immunology
  • Treatment Outcome
  • Papillomavirus Infections / veterinary
  • Sarcoidosis / veterinary
  • Sarcoidosis / therapy
  • Sarcoidosis / immunology

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 56 references
  1. Goodrich L, Gerber H, Marti E, Antczak D.F. Equine Sarcoids. Vet. Clin. N. Am. Equine Pract. 1998;14:607–623.
    doi: 10.1016/S0749-0739(17)30189-Xpubmed: 9891727google scholar: lookup
  2. Hollis A.R. Management of Equine Sarcoids. Vet. J. 2023;291:105926.
    doi: 10.1016/j.tvjl.2022.105926pubmed: 36334801google scholar: lookup
  3. Ogłuszka M, Starzyński R.R, Pierzchała M, Otrocka-Domagała I, Raś A. Equine Sarcoids—Causes, Molecular Changes, and Clinicopathologic Features: A Review. Vet. Pathol. 2021;58:472–482.
    doi: 10.1177/0300985820985114pubmed: 33461443google scholar: lookup
  4. Taylor S, Haldorson G. A Review of Equine Sarcoid. Equine Vet. Educ. 2013;25:210–216.
    doi: 10.1111/j.2042-3292.2012.00411.xgoogle scholar: lookup
  5. Tamzali Y, Borde L, Rols M.P, Golzio M, Lyazrhi F, Teissie J. Successful Treatment of Equine Sarcoids with Cisplatin Electrochemotherapy: A Retrospective Study of 48 Cases. Equine Vet. J. 2012;44:214–220.
    doi: 10.1111/j.2042-3306.2011.00425.xpubmed: 21793876google scholar: lookup
  6. Byam-Cook K.L, Henson F.M, Slater J.D. Treatment of Periocular and Non-Ocular Sarcoids in 18 Horses by Interstitial Brachytherapy with Iridium-192. Vet. Rec. 2006;159:337–341.
    doi: 10.1136/vr.159.11.337pubmed: 16963712google scholar: lookup
  7. Altamura G, Strazzullo M, Corteggio A, Francioso R, Roperto F, D’Esposito M, Roperto S. O(6)-Methylguanine-DNA Methyltransferase in Equine Sarcoids: Molecular and Epigenetic Analysis. BMC Vet. Res. 2012;8:218.
    doi: 10.1186/1746-6148-8-218pmc: PMC3512464pubmed: 23140380google scholar: lookup
  8. Jindra C, Hainisch E.K, Brandt S. Immunotherapy of Equine Sarcoids—From Early Approaches to Innovative Vaccines. Vaccines 2023;11:769.
    doi: 10.3390/vaccines11040769pmc: PMC10145708pubmed: 37112681google scholar: lookup
  9. Offer K.S, Dixon C.E, Sutton D.G.M. Treatment of Equine Sarcoids: A Systematic Review. Equine Vet. J. 2024;56:12–25.
    doi: 10.1111/evj.13935pubmed: 36917551google scholar: lookup
  10. Vanselow B.A, Abetz I, Jackson A.R. BCG Emulsion Immunotherapy of Equine Sarcoid. Equine Vet. J. 1988;20:444–447.
    doi: 10.1111/j.2042-3306.1988.tb01571.xpubmed: 3215172google scholar: lookup
  11. Martens A, De Moor A, Vlaminck L, Pille F, Steenhaut M. Evaluation of Excision, Cryosurgery and Local BCG Vaccination for the Treatment of Equine Sarcoids. Vet. Rec. 2001;149:665–669.
    doi: 10.1136/vr.149.22.665pubmed: 11765322google scholar: lookup
  12. Compston P.C, Turner T, Wylie C.E, Payne R.J. Laser Surgery as a Treatment for Histologically Confirmed Sarcoids in the Horse. Equine Vet. J. 2016;48:451–456.
    doi: 10.1111/evj.12456pubmed: 25959259google scholar: lookup
  13. Klein W.R, Bras G.E, Misdorp W, Steerenberg P.A, de Jong W.H, Tiesjema R.H, Kersjes A.W, Ruitenberg E.J. Equine Sarcoid: BCG Immunotherapy Compared to Cryosurgery in a Prospective Randomised Clinical Trial. Cancer Immunol. Immunother. 1986;21:133–140.
    doi: 10.1007/BF00199861pmc: PMC11038812pubmed: 3633215google scholar: lookup
  14. Carvalho M.C.P, Almeida G.B, Vieira R.R, Oliveira A.F, Silva F.L. Imunomodulação com vacina BCG para tratamento de sarcoide em um equino. Proceedings of the XI Simpósio Internacional do Cavalo Atleta; Belo Horizonte, MG, Brazil. 11–13 May 2023.
  15. Page M.J, McKenzie J.E, Bossuyt P.M, Boutron I, Hoffmann T, Mulrow C.D, Shamseer L, Tetzlaff J.M, Akl E, Brennan S.E. The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. BMJ 2021;372:n71.
    doi: 10.1136/bmj.n71pmc: PMC8005924pubmed: 33782057google scholar: lookup
  16. Lidagoster S, Ben-David R, De Leon B, Sfakianos JP. BCG and Alternative Therapies to BCG Therapy for Non-Muscle-Invasive Bladder Cancer. Curr. Oncol. 2024;31:1063–1078.
    doi: 10.3390/curroncol31020079pmc: PMC10888316pubmed: 38392073google scholar: lookup
  17. Pereira M, Paixão E, Trajman A, de Souza RA, da Natividade MS, Pescarini JM, Pereira SM, Barreto FR, Ximenes R, Dalcomo M. The Need for Fast-Track, High-Quality and Low-Cost Studies about the Role of the BCG Vaccine in the Fight against COVID-19. Respir. Res. 2020;21:178.
    doi: 10.1186/s12931-020-01439-4pmc: PMC7351640pubmed: 32653040google scholar: lookup
  18. Hannouneh ZA, Hijazi A, Alsaleem AA, Hami S, Kheyrbek N, Tanous F, Khaddour K, Abbas A, Alshehabi Z. Novel immuno-therapeutic options for BCG-unresponsive high-risk non-muscle-invasive bladder cancer. Cancer Med. 2023;12:21944–21968.
    doi: 10.1002/cam4.6768pmc: PMC10757155pubmed: 38037752google scholar: lookup
  19. Sultan F, Ganaie BA. Comparative Oncology: Integrating Human and Veterinary Medicine. Open Vet. J. 2018;8:25–34.
    doi: 10.4314/ovj.v8i1.5pmc: PMC5806664pubmed: 29445618google scholar: lookup
  20. Youssef E, Palmer D, Fletcher B, Vaughn R. Exosomes in Precision Oncology and Beyond: From Bench to Bedside in Diagnostics and Therapeutics. Cancers 2025;17:940.
    doi: 10.3390/cancers17060940pmc: PMC11940788pubmed: 40149276google scholar: lookup
  21. Kader M, Smith AP, Guiducci C, Wonderlich ER, Normolle D, Watkins SC, Barrat FJ, Barratt-Boyes SM. Blocking TLR7- and TLR9-Mediated IFN-α Production by Plasmacytoid Dendritic Cells Does Not Diminish Immune Activation in Early SIV Infection. PLoS Pathog. 2013;9:e1003530.
    doi: 10.1371/journal.ppat.1003530pmc: PMC3723633pubmed: 23935491google scholar: lookup
  22. Pandya A, Shah Y, Kothari N, Postwala H, Shah A, Parekh P, Chorawala MR. The Future of Cancer Immunotherapy: DNA Vaccines Leading the Way. Med. Oncol. 2023;40:200.
    doi: 10.1007/s12032-023-02060-3pmc: PMC10251337pubmed: 37294501google scholar: lookup
  23. Semik E, Gurgul A, Ząbek T, Ropka-Molik K, Koch C, Mählmann K, Bugno-Poniewierska M. Transcriptome analysis of equine sarcoids. Vet. Comp. Oncol. 2017;15:1370–1381.
    doi: 10.1111/vco.12279pubmed: 27779365google scholar: lookup
  24. Verkuijl C, Smit J, Green JMH, Nordquist RE, Sebo J, Hayek MN, Hötzel MJ. Climate change, public health, and animal welfare: Towards a One Health approach to reducing animal agriculture’s climate footprint. Front. Anim. Sci. 2024;5:1281450.
    doi: 10.3389/fanim.2024.1281450google scholar: lookup
  25. Danasekaran R. One Health: A Holistic Approach to Tackling Global Health Issues. Indian J. Community Med. 2024;49:260–263.
    doi: 10.4103/ijcm.ijcm_521_23pmc: PMC11042131pubmed: 38665439google scholar: lookup
  26. Feng X, Li Z, Liu Y, Chen D, Zhou Z. CRISPR/Cas9 technology for advancements in cancer immunotherapy: From uncovering regulatory mechanisms to therapeutic applications. Exp. Hematol. Oncol. 2024;13:102.
    doi: 10.1186/s40164-024-00570-ypmc: PMC11490091pubmed: 39427211google scholar: lookup
  27. Coman MA, Marcu A, Chereches RM, Leppälä J, Van Den Broucke S. Educational Interventions to Improve Safety and Health Literacy Among Agricultural Workers: A Systematic Review. Int. J. Environ. Res. Public Health 2020;17:1114.
    doi: 10.3390/ijerph17031114pmc: PMC7037762pubmed: 32050565google scholar: lookup
  28. Chehelgerdi M, Chehelgerdi M, Allela OQB, Pecho RDC, Jayasankar N, Rao DP, Thamaraikani T, Vasanthan M, Viktor P, Lakshmaiya N. Progressing nanotechnology to improve targeted cancer treatment: Overcoming hurdles in its clinical implementation. Mol. Cancer 2023;22:169.
    doi: 10.1186/s12943-023-01865-0pmc: PMC10561438pubmed: 37814270google scholar: lookup
  29. Nogueira SA, Torres SM, Malone ED, Diaz SF, Jessen C, Gilbert S. Efficacy of imiquimod 5% cream in the treatment of equine sarcoids: A pilot study. Vet. Dermatol. 2006;17:259–265.
    doi: 10.1111/j.1365-3164.2006.00526.xpubmed: 16827669google scholar: lookup
  30. Pagliusi S, Che Y, Dong S. The art of partnerships for vaccines. Vaccine 2019;37:5909–5919.
    doi: 10.1016/j.vaccine.2019.07.088pmc: PMC6739625pubmed: 31447125google scholar: lookup
  31. Lorga AD, Amaro F, Gomes ARC, Cocco M, Gularte A, Silva Y, Silva J. Application of Euphorbia tirucalli Sap in Sarcoid Treatment in Horses—Case Report. Arq. Bras. Med. Vet. Zootec. 2022;74:509–513.
    doi: 10.1590/1678-4162-12487google scholar: lookup
  32. Knottenbelt DC, Kelly DF. The Diagnosis and Treatment of Periorbital Sarcoid in the Horse: 445 Cases from 1974 to 1999. Vet. Ophthalmol. 2000;3:169–191.
    doi: 10.1046/j.1463-5224.2000.00119.xpubmed: 11397301google scholar: lookup
  33. Knottenbelt DC. The Equine Sarcoid: Why Are There So Many Treatment Options?. Vet. Clin. N. Am. Equine Pract. 2019;35:243–262.
    doi: 10.1016/j.cveq.2019.03.006pubmed: 31097356google scholar: lookup
  34. Brum JS, Souza TM, Barros CSL. Aspectos epidemiológicos e distribuição anatômica das diferentes formas clínicas do sarcoide equino no Rio Grande do Sul: 40 casos. Pesqui. Vet. Bras. 2010;30:839–843.
    doi: 10.1590/S0100-736X2010001000006google scholar: lookup
  35. Anjos BL, Silva MS, Diefenbach A, Brito MF, Seppa GS, Brum MCS. Sarcoide equino associado ao papilomavírus bovino BR-UEL-4. Ciênc. Rural. 2010;40:1456–1459.
    doi: 10.1590/S0103-84782010000600037google scholar: lookup
  36. Alcântara BK, Alfieri AA, Headley SA, Rodrigues WB, Otonel RAA, Lunardi M, Alfieri AF. Caracterização molecular de DNA de Delta papillomavirus bovino (BPV1, 2–13) em sarcoides equinos. Pesqui. Vet. Bras. 2015;35:431–436.
    doi: 10.1590/S0100-736X2015000500007google scholar: lookup
  37. Munday JS, Orbell G, Fairley RA, Hardcastle M, Vaatstra B. Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA are Detectable in around 10% of Sarcoids. Animals 2021;11:3093.
    doi: 10.3390/ani11113093pmc: PMC8614326pubmed: 34827825google scholar: lookup
  38. Karalus W, Subharat S, Orbell G, Vaatstra B, Munday JS. Equine Sarcoids: A Clinicopathologic Study of 49 Cases, with Mitotic Count and Clinical Type Predictive of Recurrence. Vet. Pathol. 2024;61:357–365.
    doi: 10.1177/03009858231209408pmc: PMC11067406pubmed: 37937724google scholar: lookup
  39. Bogaert L, Martens A, Van Poucke M, Ducatelle R, De Cock H, Dewulf J, De Baere C, Peelman L, Gasthuys F. High prevalence of bovine papillomaviral DNA in the normal skin of equine sarcoid-affected and healthy horses. Vet. Microbiol. 2008;129:58–68.
    doi: 10.1016/j.vetmic.2007.11.008pubmed: 18093754google scholar: lookup
  40. McConaghy FF, Davis RE, Reppas GP, Rawlinson RJ, McClintock SA, Hutchins DR, Hodgson DR. Management of equine sarcoids: 1975–1993. N. Z. Vet. J. 1994;42:180–184.
    doi: 10.1080/00480169.1994.35816pubmed: 16031776google scholar: lookup
  41. Horefti E. The Importance of the One Health Concept in Combating Zoonoses. Pathogens 2023;12:977.
    doi: 10.3390/pathogens12080977pmc: PMC10460008pubmed: 37623937google scholar: lookup
  42. Stewart AA, Rush B, Davis E. The efficacy of intratumoural 5-fluorouracil for the treatment of equine sarcoids. Aust. Vet. J. 2006;84:101–106.
    doi: 10.1111/j.1751-0813.2006.tb12239.xpubmed: 16555558google scholar: lookup
  43. Stadler S, Kainzbauer C, Haralambus R, Brehm W, Hainisch E, Brandt S. Successful treatment of equine sarcoids by topical aciclovir application. Vet. Rec. 2011;168:187.
    doi: 10.1136/vr.c5430pubmed: 21493530google scholar: lookup
  44. Walker M, Adams W, Hoskinson J, Held JP, Blackford J, Geiser D, Henton J. Iridium-192 brachytherapy for equine sarcoid, one and two year remission rates. Vet. Radiol. 1991;32:206–208.
    doi: 10.1111/j.1740-8261.1991.tb00108.xgoogle scholar: lookup
  45. Spoormakers TJ, Klein WR, Jacobs JJ, Van Den Ingh TS, Koten JW, Den Otter W. Comparison of the efficacy of local treatment of equine sarcoids with IL-2 or cisplatin/IL-2. Cancer Immunol. Immunother. 2003;52:179–184.
    doi: 10.1007/s00262-002-0369-0pmc: PMC11032927pubmed: 12649747google scholar: lookup
  46. Berruex F, Gerber V, Wohlfender FD, Burger D, Koch C. Clinical course of sarcoids in 61 Franches-Montagnes horses over a 5–7 year period. Vet. Q. 2016;36:189–196.
    doi: 10.1080/01652176.2016.1204483pubmed: 27327513google scholar: lookup
  47. Assis-Brasil ND, Marcolongo-Pereira C, Stigger AL, Fiss L, Santos BL, Coelho ACB, Sallis ESV, Fernandes CG, Schild AL. Equine Dermatopathies in Southern Brazil: A Study of 710 Cases. Ciênc. Rural. 2015;45:519–524.
    doi: 10.1590/0103-8478cr20140901google scholar: lookup
  48. Baust JG, Gage AA. The Molecular Basis of Cryosurgery. BJU Int. 2005;95:1187–1191.
    doi: 10.1111/j.1464-410X.2005.05502.xpubmed: 15892798google scholar: lookup
  49. Schaffer PA, Wobeser B, Martin LE, Dennis MM, Duncan CG. Cutaneous Neoplastic Lesions of Equids in the Central United States and Canada: 3,351 Biopsy Specimens from 3,272 Equids (2000–2010). J. Am. Vet. Med. Assoc. 2013;242:99–104.
    doi: 10.2460/javma.242.1.99pubmed: 23234288google scholar: lookup
  50. Ireland JL, Wylie CE, Collins SN, Verheyen KL, Newton JR. Preventive Health Care and Owner-Reported Disease Prevalence of Horses and Ponies in Great Britain. Res. Vet. Sci. 2013;95:418–424.
    doi: 10.1016/j.rvsc.2013.05.007pubmed: 23768693google scholar: lookup
  51. Aminin D, Wang YM. Macrophages as a “Weapon” in Anticancer Cellular Immunotherapy. Kaohsiung J. Med. Sci. 2021;37:749–758.
    doi: 10.1002/kjm2.12405pmc: PMC11896134pubmed: 34110692google scholar: lookup
  52. Perez-Penco M, Byrdal M, Lara de la Torre L, Ballester M, Khan S, Siersbæk M, Lecoq I, Madsen CO, Kjeldsen JW, Svane IM. The Antitumor Activity of TGFβ-Specific T Cells Is Dependent on IL-6 Signaling. Cell Mol. Immunol. 2025;22:111–126.
    doi: 10.1038/s41423-024-01238-7pmc: PMC11685413pubmed: 39653766google scholar: lookup
  53. Collinet A, Grimm P, Jacotot E, Julliand V. Biomarkers for monitoring the equine large intestinal inflammatory response to stress-induced dysbiosis and probiotic supplementation. J. Anim. Sci. 2022;100:skac268.
    doi: 10.1093/jas/skac268pmc: PMC9576022pubmed: 35980768google scholar: lookup
  54. Kober AKMH, Riaz Rajoka MS, Mehwish HM, Villena J, Kitazawa H. Immunomodulation Potential of Probiotics: A Novel Strategy for Improving Livestock Health, Immunity, and Productivity. Microorganisms 2022;10:388.
    doi: 10.3390/microorganisms10020388pmc: PMC8878146pubmed: 35208843google scholar: lookup
  55. Ahmed MM, Okesanya OJ, Othman ZK, Ibrahim AM, Adigun OA, Ukoaka BM, Abdi MI, Lucero-Prisno DE. Holistic Approaches to Zoonoses: Integrating Public Health, Policy, and One Health in a Dynamic Global Context. Zoonotic Dis. 2025;5:5.
    doi: 10.3390/zoonoticdis5010005google scholar: lookup
  56. Singh S, Saavedra-Avila NA, Tiwari S, Porcelli SA. A Century of BCG Vaccination: Immune Mechanisms, Animal Models, Non-Traditional Routes and Implications for COVID-19. Front. Immunol. 2022;13:959656.
    doi: 10.3389/fimmu.2022.959656pmc: PMC9459386pubmed: 36091032google scholar: lookup

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