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Home / Equine Research Bank / Front Vet Sci / Berbamine, a bioactive alkaloid, suppresses equine herpesvir...
Frontiers in veterinary science2023; 10; 1163780; doi: 10.3389/fvets.2023.1163780

Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo.

Abstract: Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 infection. Quantitative PCR (qPCR), immunoblotting, the Reed-Muench method, and pathological examination were employed to study the ability of BBM to inhibit EHV-1 infection, viral DNA replication, viral protein production, virion secretion, and cytopathogenesis in vitro and in vivo. The in vitro studies revealed that 10 μM BBM effectively suppressed EHV-1 viral entry into cells, viral DNA replication, and virion secretion, while the in vivo studies verified the ability of BBM to suppress EHV-1-induced damage of brain and lung tissues and animal mortality. These findings strongly suggest that BBM could be a serious contender in the therapeutic control of EHV-1 infection of equines.
Copyright © 2023 Li, He, Ge, Quan, Chen, Hu, Sa, Liu, Ran, Fu and Shi.
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Publication Date: 2023-05-25 PubMed ID: 37303732PubMed Central: PMC10247970DOI: 10.3389/fvets.2023.1163780Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the impact of berbamine (BBM), a bioactive alkaloid, on equine herpesvirus type 1 (EHV-1). Findings suggest that BBM inhibits the virus’ entry into cells, prevents replication of viral DNA, limits virion secretion, and reduces EHV-1-induced damage to brain and lung tissues in equines.

Objective and Significance of the Study

  • The study was led by the necessity to discover a new method to control EHV-1, a virus harmful to horses worldwide.
  • The research focuses on berbamine (BBM), a substance already identified as having anti-viral properties. Its effects against EHV-1, however, hadn’t been thoroughly studied.
  • Understanding of BBM’s properties could offer considerable benefit in formulating a viable treatment against EHV-1.

Methodologies Employed

  • The experiment involved both and studies to evaluate BBM’s effectiveness against EHV-1 effectively.
  • Quantitative PCR (qPCR) and immunoblotting were used to measure how BBM can prevent the EHV-1 virus from entering equine cells and replicate its DNA inside.
  • The Reed-Muench method was utilized to assess the virion secretion – the process of releasing new virus particles from the host cell.
  • Pathological examinations confirmed the damage caused by EHV-1 on equine brain and lung tissues. They provided empirical evidence to set a baseline for assessing the impact of the BBM treatment.

Findings and Conclusion

  • The study discovered that 10 µM BBM substantially impedes the entry of EHV-1 into equine cells, the replication of its DNA, and the secretion of virion.
  • Additional studies validated BBM’s potential to reduce EHV-1-related damage to brain and lung tissues and to decrease animal mortality.
  • The findings strongly indicate that BBM could be a strong candidate in the development of an effective EHV-1 treatment for horses.

Cite This Article

APA
Li Z, He Y, Ge L, Quan R, Chen J, Hu Y, Sa R, Liu J, Ran D, Fu Q, Shi H. (2023). Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo. Front Vet Sci, 10, 1163780. https://doi.org/10.3389/fvets.2023.1163780

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 10
Pages: 1163780

Researcher Affiliations

Li, Zeyu
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
He, Yuanxiu
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Ge, Lijuan
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Quan, Ran
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Chen, Junzhen
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Hu, Yue
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Sa, Ruixue
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Liu, Jianhua
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Ran, Duoliang
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Fu, Qiang
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Shi, Huijun
  • College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships.

References

This article includes 33 references
  1. Walter J, Balzer HJ, Seeh C, Fey K, Bleul U, Osterrieder N. Venereal shedding of equid herpesvirus-1 (EHV-1) in naturally infected stallions.. J Vet Intern Med (2012) 26:1500–4.
    doi: 10.1111/j.1939-1676.2012.00997.xpubmed: 22947047google scholar: lookup
  2. Sutton G, Garvey M, Cullinane A, Marcillaud PC. Molecular surveillance of EHV-1 strains circulating in France during and after the major 2009 outbreak in Normandy involving respiratory infection, neurological disorder, and abortion.. Viruses (2019) 11:916.
    doi: 10.3390/v11100916pmc: PMC6832873pubmed: 31590336google scholar: lookup
  3. Oladunni FS, Horohov DW, Chambers TM. EHV-1: a constant threat to the horse industry.. Front Microbiol (2019) 10:2668.
    doi: 10.3389/fmicb.2019.02668pmc: PMC6901505pubmed: 31849857google scholar: lookup
  4. Stokol T, Soboll HG. Current research in equid herpesvirus Type-1 (EHV-1).. Front Vet Sci (2020) 6:492.
    doi: 10.3389/fvets.2019.00492pmc: PMC6965053pubmed: 31998768google scholar: lookup
  5. Zarski LM, Vaala WE, Barnett DC, Gisela SH. A live-attenuated equine influenza vaccine stimulates innate immunity in equine respiratory epithelial cell cultures that could provide protection from equine herpesvirus 1.. Front Vet Sci (2021) 8:674850.
    doi: 10.3892/or_00000688pmc: PMC8224402pubmed: 34179166google scholar: lookup
  6. Sutton G, Normand C, Carnet F, Couroucé A, Garvey M, Castagnet S. Equine herpesvirus 1 variant and new marker for epidemiologic surveillance, Europe, 2021.. Emerg Infect Dis (2021) 27:2738–9.
    doi: 10.3201/eid2710.210704pmc: PMC8462333pubmed: 34546162google scholar: lookup
  7. Ma G, Eschbaumer M, Said A, Hoffmann B, Beer M, Osterrieder N. An equine herpesvirus type 1 (EHV-1) expressing VP2 and VP5 of serotype 8 bluetongue virus (BTV-8) induces protection in a murine infection model.. PLoS One (2012) 7:e34425.
    doi: 10.1371/journal.pone.0034425pmc: PMC3325243pubmed: 22511939google scholar: lookup
  8. Mc FA, Hanlon D, McKenzie RK, Mayhew I. The first reported outbreak of equine herpesvirus myeloencephalopathy in New Zealand.. N Z Vet J (2016) 64:125–34.
    doi: 10.1080/00480169.2015.1096853pubmed: 26414406google scholar: lookup
  9. Si YL, Li HL, Teh BS, Thong Y. Anti-inflammatory and immunosuppressive properties of the bis-benzylisoquinolines: in vitro comparisons of tetrandrine and berbamine.. Int J Immunopharmacol (1989) 11:395–401.
    doi: 10.1016/0192-0561(89)90086-6pubmed: 2777433google scholar: lookup
  10. Liu L, Liang D, Zheng Q, Zhao M, Lv R, Tang J. Berbamine dihydrochloride suppresses the progression of colorectal cancer via RTKs/Akt axis.. J Ethnopharmacol (2023) 303:116025.
    doi: 10.1016/j.jep.2022.116025pubmed: 36496042google scholar: lookup
  11. Zhao Y, Tan Y, Wu G, He H. Berbamine overcomes imatinib-induced neutropenia and permits cytogenetic responses in Chinese patients with chronic-phase chronic myeloid leukemia.. Int J Hematol (2011) 94:156–62.
    doi: 10.1007/s12185-011-0887-7pubmed: 21728004google scholar: lookup
  12. Liu L, Yan J, Cao Y, Shusheng W. Proliferation, migration and invasion of triple negative breast cancer cells are suppressed by berbamine via the PI3K/Akt/MDM2/p53 and PI3K/Akt/mTOR signaling pathways.. Oncol Lett (2021) 21:70–1.
    doi: 10.3892/ol.2020.12331pmc: PMC7716707pubmed: 33365081google scholar: lookup
  13. Huang L, Li H, Ye Z, Yue J. Berbamine inhibits Japanese encephalitis virus (JEV) infection by compromising TPRMLs-mediated endolysosomal trafficking of low-density lipoprotein receptor (LDLR).. Emerg Microbes Infect (2021) 10:1257–71.
    doi: 10.1080/22221751.2021.1941276pmc: PMC8238074pubmed: 34102949google scholar: lookup
  14. Cen S, Yi D, Li Q, Shan C. Repurposing of berbamine hydrochloride to inhibit Ebola virus by targeting viral glycoprotein.. Acta Pharm Sin B (2022) 12:4378–89.
    doi: 10.1016/j.apsb.2022.05.023pmc: PMC9764067pubmed: 36561997google scholar: lookup
  15. Mesquita LP, Arévalo AF, Zanatto DA, Miyashiro SI, Cunha EMS, Souza MDCC. Equine herpesvirus type 1 induces both neurological and respiratory disease in Syrian hamsters.. Vet Microbiol (2017) 203:117–24.
    doi: 10.1016/j.vetmic.2017.03.007pubmed: 28619133google scholar: lookup
  16. Saleh AG, El HN, Abd HA, Abas OM, Anwar S, Fukushi H. Comparative study of the pathogenesis of Rhinopneumonitis induced by intranasal inoculation of hamsters with equine Herpesvirus-9, equine Herpesvirus-1 strain Ab4p and Zebra-borne equine Herpesvirus-1.. J Comp Pathol (2020) 180:35–45.
    doi: 10.1016/j.jcpa.2020.08.002pubmed: 33222872google scholar: lookup
  17. Zhang D, Bai C, Ge K, Yong W. Establishment of an SYBR green-based real-time PCR assay for porcine circovirus type 4 detection.. J Virol Methods (2020) 285:113963.
    doi: 10.1016/j.jviromet.2020.113963pubmed: 32882322google scholar: lookup
  18. Zhu J, Huang L, Gao F, Jian W, Chen H, Liao M. Berbamine hydrochloride inhibits African swine fever virus infection in vitro.. Molecules (2022) 28:170.
    doi: 10.3390/molecules28010170pmc: PMC9822360pubmed: 36615369google scholar: lookup
  19. Grela E, Kozłowska J, Grabowiecka A. Current methodology of MTT assay in bacteria–a review.. Acta Histochem (2018) 120:303–11.
    doi: 10.1016/j.acthis.2018.03.007pubmed: 29606555google scholar: lookup
  20. Álvarez D, Muñoz AL, Tavera RP, Marcela MR. Low neutralizing antibody titers against the mu variant of SARS-CoV-2 in 31 BNT162b2 vaccinated individuals in Colombia.. Vaccine (2022) 10:180.
    doi: 10.3390/vaccines10020180pmc: PMC8876570pubmed: 35214639google scholar: lookup
  21. Ran D L, Hu Y, Liu J H. Application of Equine Herpesvirus Type 1.. (2020).
  22. Beurden SJ, Voorbergen MA, Roozenburg I, Boerlage AS, Haenen OL, Engelsma MY. Development and validation of a two-step real-time RT-PCR for the detection of eel virus European X in European eel, Anguilla anguilla.. J Virol Methods (2011) 171:352–9.
    doi: 10.1016/j.jviromet.2010.11.019pubmed: 21126538google scholar: lookup
  23. Hu Y, Jia Q, Liu J, Ran D. Molecular characteristics and pathogenicity of an equid alphaherpesvirus 1 strain isolated in China.. Virus Genes (2022) 58:284–93.
    doi: 10.1007/s11262-022-01910-ypubmed: 35567668google scholar: lookup
  24. Chan CM, Chu H, Zhang AJ, Kwok YY. Hemagglutinin of influenza a virus binds specifically to cell surface nucleolin and plays a role in virus internalization.. Virology (2016) 494:78–88.
    doi: 10.1016/j.virol.2016.04.008pubmed: 27085069google scholar: lookup
  25. Lee K, Han DG, Kim S, Choi E, Choi KS. Experimental infection of mice with noncytopathic bovine viral diarrhea virus 2 increases the number of megakaryocytes in bone marrow.. Virol J (2018) 15:115–9.
    doi: 10.1186/s12985-018-1030-7pmc: PMC6064063pubmed: 30055639google scholar: lookup
  26. Seong G, Oem JK, Lee KH, Choi KS. Experimental infection of mice with bovine viral diarrhea virus.. Arch Virol (2015) 160:1565–71.
    doi: 10.1007/s00705-015-2412-4pubmed: 25850760google scholar: lookup
  27. Huang L, Yuen TTT, Ye Z, Yue J. Berbamine inhibits SARS-CoV-2 infection by compromising TRPMLs-mediated endolysosomal trafficking of ACE2.. Signal Transduct Target Ther (2021) 6:168–3.
    doi: 10.1038/s41392-021-00584-6pmc: PMC8065329pubmed: 33895782google scholar: lookup
  28. Tong Q, Liu G, Sang X, Zhu X, Fu X, Dou C. Targeting RNA G-quadruplex with repurposed drugs blocks SARS-CoV-2 entry.. PLoS Pathog (2023) 19:e1011131.
    doi: 10.1371/journal.ppat.1011131pmc: PMC9904497pubmed: 36701392google scholar: lookup
  29. Braga L, Ali H, Secco I, Chiavacci E, Neves G, Goldhill D. Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia.. Nature (2021) 594:88–93.
    doi: 10.1038/s41586-021-03491-6pmc: PMC7611055pubmed: 33827113google scholar: lookup
  30. Wang J, Yang G, Zhang L, Zhang J, Wang J, Zou Y. Berbamine hydrochloride inhibits bovine viral diarrhea virus replication via interfering in late-stage autophagy.. Virus Res (2022) 321:198905.
    doi: 10.1016/j.virusres.2022.198905pubmed: 36064041google scholar: lookup
  31. Stokes A, Allen GP, Pullen LA, Murray PK. A hamster model of equine herpesvirus type 1 (EHV-1) infection; passive protection by monoclonal antibodies to EHV-1 glycoproteins 13, 14 and 17/18.. J Gen Virol (1989) 70:1173–83.
    doi: 10.1099/0022-1317-70-5-1173pubmed: 2471805google scholar: lookup
  32. Awan AR, Chong YC, Field HJ. The pathogenesis of equine herpesvirus type 1 in the mouse: a new model for studying host responses to the infection.. J Gen Virol (1990) 71:1131–40.
    doi: 10.1099/0022-1317-71-5-1131pubmed: 2161048google scholar: lookup
  33. Kasem S, Yu MHH, Yamada S, Hideto F. The ORF37 (UL24) is a neuropathogenicity determinant of equine herpesvirus 1 (EHV-1) in the mouse encephalitis model.. Virology (2010) 400:259–70.
    doi: 10.1016/j.virol.2010.02.012pubmed: 20199788google scholar: lookup
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