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Binding of cellular proteins to the leader RNA of equine arteritis virus.
Abstract: The genome of equine arteritis virus (EAV) produces a 3' coterminal-nested set of six subgenomic (sg) viral RNAs during virus replication cycle, and each set possesses a common leader sequence of 206 nucleotides (nt) in length derived from the 5' end of the viral genome. Given the presence of the leader region within both genomic and sg mRNAs, it is likely to contain cis-acting signals that may interact with cellular or viral proteins for RNA synthesis. Gel mobility shift assays indicated that proteins in Vero cell cytoplasmic extracts formed complexes with the positive (+) and negative (-) strands of the EAV leader RNA. Several cell proteins with molecular masses ranging from 74 to 31 kDa and 58 to 32 kDa were detected in UV-induced cross-linking assays with the EAV leader RNA (+) and (-) strands, respectively. In both cases, intense bands were observed at the 58-52 kDa molecular weight markers. Results from competition gel mobility shift assays using overlapping cold RNA probes spanning the leader RNA (+) strand indicated that nt 140-206 are not necessary for binding to cell proteins.
Publication Date: 2005-03-04 PubMed ID: 15744570PubMed Central: PMC7088659DOI: 10.1007/s11262-004-4589-6Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research study explores how cellular proteins bind to the leader RNA of the equine arteritis virus (EAV). It investigated if certain cell proteins form complexes with the EAV leader RNA, with the ultimate goal of understanding how the EAV replication cycle works.
Objectives and Methodology
- The underlying objective of this research was to decipher the role of ‘leader RNA’ in the replication of the equine arteritis virus (EAV).
- The study presumed that the leader RNA of EAV, common to both genomic and subgenomic RNAs, likely carries cis-acting signals to facilitate the RNA synthesis by interacting with certain cellular or viral proteins.
- The researchers utilized gel mobility shift assays to demonstrate that proteins in Vero cell cytoplasmic extracts formed complexes with the positive and negative strands of the EAV leader RNA.
- UV-induced cross-linking assays were used to ascertain the association of proteins of varying molecular masses (between 74 to 31 kDa and 58 to 32 kDa), with the EAV leader RNA.
Key Findings
- The research discovered that some cell proteins with molecular masses ranging from 74 to 31 kDa for the positive strands, and 58 to 32 kDa for the negative strands, were detected in the UV-induced cross-linking assays with EAV leader RNA.
- Notably, intense bands were observed at the 58-52 kDa molecular weight markers for both the positive and negative strands, indicating significant binding occurring at this level.
- Observations from gel mobility shift assays using overlapping cold RNA probes to span the leader RNA positive strand showed that nucleotides 140-206 are not necessary for binding to cell proteins.
- The research implies that binding to cellular proteins, important for virus replication, can occur even in the absence of nucleotides 140-206, a discovery that can further applications in designing effective antiviral strategies.
Implications of the Research
- This study provides a clearer understanding of the replication mechanism of the EAV. The findings can form the foundation for developing therapeutic strategies against equine arteritis virus.
- Deeper understanding of protein-RNA binding patterns in virus replication may facilitate progress in designing RNA-targeted antiviral drugs or treatment mechanisms.
Cite This Article
APA
Archambault D, St-Louis MC, Martin S.
(2005).
Binding of cellular proteins to the leader RNA of equine arteritis virus.
Virus Genes, 30(1), 121-125.
https://doi.org/10.1007/s11262-004-4589-6 Publication
Researcher Affiliations
- Department of Biological Sciences, University of Québec at Montréal, P.O. Box 8888, Succursale Centre-Ville, Montréal, Québec, H3C 3P8, Canada. archambault.denis@uqam.ca
MeSH Terms
- 5' Untranslated Regions / metabolism
- Electrophoretic Mobility Shift Assay
- Equartevirus / genetics
- Equartevirus / metabolism
- Molecular Weight
- Protein Binding
- Proteins / chemistry
- Proteins / isolation & purification
- Proteins / metabolism
- RNA Probes
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This article includes 19 references
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Citations
This article has been cited 2 times.- Zhong Y, Tan YW, Liu DX. Recent progress in studies of arterivirus- and coronavirus-host interactions. Viruses 2012 Jun;4(6):980-1010.
- Archambault D, Kheyar A, de Vries AA, Rottier PJ. The intraleader AUG nucleotide sequence context is important for equine arteritis virus replication. Virus Genes 2006 Aug;33(1):59-68.
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