Binding of epidermal growth factor and transforming growth factor-alpha in mammalian preimplantation embryos.
Abstract: Preimplantation embryos of the pig (Days 11 to 15), cow (Days 14 to 16), sheep (Day 14) and pony (Day 16) bind epidermal growth factor (EGF) specifically. Binding was not detected in embryos of the rabbit at Day 5 or 6 or the hamster at Day 3. Transforming growth factor-alpha displaced [(125)I] EGF in pig, cow and pony embryos almost as much as unlabeled EGF. The binding affinities of EGF ranged from 12 to 233 pM in pig and cow embryos. The range of species and binding features indicate that the EGF family may play a significant role in mammalian preimplantation development.
Publication Date: 1994-01-01 PubMed ID: 16727441DOI: 10.1016/0093-691x(94)90503-bGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research article explores the binding of epidermal growth factor (EGF) and transforming growth factor-alpha in preimplantation embryos of various mammals and suggests that the EGF family may have a significant role in early mammalian development.
Research Focus
The main purpose of this research was to study and understand the binding behavior of two factors – Epidermal Growth Factor (EGF) and Transforming Growth Factor-alpha – in preimplantation embryos of different mammals. The selected mammals for this study include pigs, cows, sheep, ponies, rabbits, and hamsters. The research also aimed at deciphering whether there is any possible role of the EGF family in early mammalian development.
- The researchers chose embryos at different development stages: Days 11 to 15 for pigs, Days 14 to 16 for cows, Day 14 for sheep, Day 16 for ponies, Day 5 or 6 for rabbits, and Day 3 for hamsters.
- EFG and Transforming Growth Factor-alpha were evaluated for their binding potentials.
Key Findings
Several significant observations were made during this research.
- Specific binding of EGF was observed in the preimplantation embryos of pigs, cows, sheep, and ponies, but not in rabbits and hamsters.
- The Transforming Growth Factor-alpha was able to displace EGF in pig, cow, and pony embryos with efficiency similar to that of unlabeled EGF, indicating a potential interplay between the two factors.
- The binding affinities of EGF varied among the embryos, with a range from 12 to 233 pM in pig and cow embryos.
Implications
The results of this study open up a range of possibilities for further research.
- The fact that specific EGF binding was observed in certain mammals but not others could suggest a species-specific role of EGF in embryonic development.
- The ability of Transforming Growth Factor-alpha to displace EGF in some species could imply coordinated roles of these factors.
- The difference in binding affinities further suggests that each species may have a unique response to EGF.
- Overall, these findings suggest a potential influential role of the EGF family in the preimplantation development of mammals.
Cite This Article
APA
Fischer B, Rose-Hellekant TA, Sheffield LG, Bertics PJ, Bavister BD.
(1994).
Binding of epidermal growth factor and transforming growth factor-alpha in mammalian preimplantation embryos.
Theriogenology, 41(4), 879-887.
https://doi.org/10.1016/0093-691x(94)90503-b Publication
Researcher Affiliations
- Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI 53706, USA.
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