Biochemical and genetic characterization of Corynebacterium pseudotuberculosis.
Abstract: Isolates (n = 94) of Corynebacterium pseudotuberculosis were obtained from sheep, goats, horses, and cattle from various parts of the world. The isolates were characterized biochemically and by restriction endonuclease analysis of DNA. We found near homogeneity in the ability of isolates to ferment carbohydrates and to produce urease. All isolates produced phospholipase D and catalase. The ability of isolates from horses to reduce nitrate, the inability of isolates from sheep and goats to do so, and the correlation of this characteristic with results of restriction endonuclease analyses confirmed the existence of 2 biovars of C pseudotuberculosis. We propose that these biovars be referred to as biovar equi for isolates that reduce nitrate and biovar ovis for isolates that fail to do so.
Publication Date: 1988-02-01 PubMed ID: 2831763
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study involves the biochemical and genetic characterization of different strains of the bacterium Corynebacterium pseudotuberculosis. The researchers concluded that there are two distinct groups within this species, marked by differences in their ability to reduce nitrate.
Research Material
- The researchers used 94 isolates of Corynebacterium pseudotuberculosis derived from sheep, goats, horses, and cattle from various parts of the world for their research.
Investigation Method
- These isolates were characterized through two different methods, one being biochemical analysis and the other being the analysis of DNA through a process called restriction endonuclease.
Key Findings
- There was found to be near homogeneity in the ability of isolates to ferment carbohydrates and to produce certain enzymes namely, urease, phospholipase D and catalase.
- However, a key discovery was made regarding the ability of different isolates to reduce nitrate. Specifically, researchers found that isolates from horses were capable of reducing nitrate while those from sheep and goats were not.
- This key distinction, coupled with results from restriction endonuclease analyses, allowed the researchers to assert the existence of two biological varieties (biovars) within the Corynebacterium pseudotuberculosis species.
Proposed Categorization
- In light of these findings, the authors proposed that the two groups should be referred to as biovar equi (for the isolates capable of nitrate reduction) and biovar ovis (for those that are incapable of nitrate reduction).
Cite This Article
APA
Songer JG, Beckenbach K, Marshall MM, Olson GB, Kelley L.
(1988).
Biochemical and genetic characterization of Corynebacterium pseudotuberculosis.
Am J Vet Res, 49(2), 223-226.
Publication
Researcher Affiliations
- Department of Veterinary Science, College of Agriculture, University of Arizona, Tucson 85721.
MeSH Terms
- Animals
- Carbohydrate Metabolism
- Catalase / biosynthesis
- Cattle
- Corynebacterium / classification
- Corynebacterium / enzymology
- Corynebacterium / genetics
- Corynebacterium / metabolism
- DNA Restriction Enzymes
- DNA, Bacterial / analysis
- Goats
- Horses
- Nitrate Reductase
- Nitrate Reductases / metabolism
- Nitrates / metabolism
- Oxidation-Reduction
- Phospholipase D / biosynthesis
- Sheep
- Urease / biosynthesis
Grant Funding
- S07 RR07002 / NCRR NIH HHS
Citations
This article has been cited 23 times.- Schlicher J, Schmitt S, Stevens MJA, Stephan R, Ghielmetti G. Molecular Characterization of Corynebacterium pseudotuberculosis Isolated over a 15-Year Period in Switzerland. Vet Sci 2021 Jul 30;8(8).
- Barral TD, Mariutti RB, Arni RK, Santos AJ, Loureiro D, Sokolonski AR, Azevedo V, Borsuk S, Meyer R, Portela RD. A panel of recombinant proteins for the serodiagnosis of caseous lymphadenitis in goats and sheep. Microb Biotechnol 2019 Nov;12(6):1313-1323.
- Droppa-Almeida D, Franceschi E, Padilha FF. Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis. Bioinform Biol Insights 2018;12:1177932218755337.
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- Dorneles EM, Santana JA, Ribeiro D, Dorella FA, Guimarães AS, Moawad MS, Selim SA, Garaldi AL, Miyoshi A, Ribeiro MG, Gouveia AM, Azevedo V, Heinemann MB, Lage AP. Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates. PLoS One 2014;9(6):e98758.
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