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Journal of bacteriology1966; 92(1); 250-257; doi: 10.1128/jb.92.1.250-257.1966

Biological and morphological aspects of the growth of equine abortion virus.

Abstract: Darlington, R. W. (St. Jude Children's Research Hospital, Memphis, Tenn.), and C. James. Biological and morphological aspects of the growth of equine abortion virus. J. Bacteriol. 92:250-257. 1966.-The growth of equine abortion virus (EAV) was studied by bioassay and electron microscopy in L-cell monolayer and suspension cultures, and in HeLa and BHK 21/13 cell monolayers. Results of virus assay (plaque-forming units) indicated that production of cell-associated virus (CAV) began at 6 to 9 hr after infection in all of the cell strains used. Virus release occurred 1 to 2 hr later. By 15 to 20 hr after infection, the amount of released virus (RV) equaled or surpassed that of CAV in all cells other than the HeLa cells, where the amount of RV did not equal CAV until 48 hr after infection. Electron microscopy of infected cells revealed no differences in the morphology of virus development in any of the cells used. Developing virus particles were first detected in cell nuclei at 9 hr after infection. At 12 hr, virus particles could be seen budding from the inner nuclear envelope. Budding into cytoplasmic vacuoles was not seen. Budding virus, virus in cytoplasmic vacuoles, and extracellular virus were all approximately 145 mmu in diameter, and were indistinguishable morphologically. These results indicated that EAV is quite similar to herpes simplex virus with respect to growth and morphology, and that the inner nuclear membrane is the principal site of virus envelopment.
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Publication Date: 1966-07-01 PubMed ID: 5941279PubMed Central: PMC276222DOI: 10.1128/jb.92.1.250-257.1966Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores the growth patterns and characteristics of the equine abortion virus (EAV) using bioassay and electron microscopy in various cell cultures. The results indicate EAV shows significant resemblance to the herpes simplex virus in terms of growth and morphology.

Methodology

  • The study used L-cell monolayer and suspension cultures, as well as HeLa and BHK 21/13 cell monolayers as its subjects.
  • It leveraged both bioassay and electron microscopy techniques to investigate the growth of the equine abortion virus (EAV).

Results and Findings

  • The production of cell-associated virus (CAV) began between 6 to 9 hours after infection in every cell strain used in the study.
  • The release of the virus was said to occur 1 to 2 hours after the initiation of the formation of the CAV.
  • The study found that by 15 to 20 hours after infection, the amount of released virus (RV) was either equal to or greater than CAV in all cell strains except HeLa cells. For HeLa cells, RV did not equal CAV until 48 hours post-infection.
  • The electron microscopy technique helped the researchers observe the development of virus particles within the cell nuclei. The first signs of developing virus particles were noticed at about 9 hours post-infection.
  • Around the 12th hour, the virus particles were seen budding from the inner nuclear envelope. Bud-offs into cytoplasmic vacuoles were not observed.
  • All the virus forms – budding, within cytoplasmic vacuoles, and extracellular were approximately 145 nm in diameter and shared the same morphological characteristics.

Conclusions

  • The outcomes of this research point towards the strong resemblance of EAV to the herpes simplex virus in relation to its growth attributes and morphological characteristics.
  • This study has deduced that the primary site for virus envelopment is the inner nuclear membrane.

Cite This Article

APA
Darlington RW, James C. (1966). Biological and morphological aspects of the growth of equine abortion virus. J Bacteriol, 92(1), 250-257. https://doi.org/10.1128/jb.92.1.250-257.1966

Publication

Journal of bacteriology
ISSN: 0021-9193
NlmUniqueID: 2985120R
Country: United States
Language: English
Volume: 92
Issue: 1
Pages: 250-257

Researcher Affiliations

Darlington, R W
    James, C

      MeSH Terms

      • Animals
      • HeLa Cells
      • Herpesviridae / growth & development
      • Humans
      • In Vitro Techniques
      • L Cells
      • Mice
      • Microscopy, Electron

      References

      This article includes 20 references
      1. DULBECCO R, VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses.. J Exp Med 1954 Feb;99(2):167-82.
        pubmed: 13130792doi: 10.1084/jem.99.2.167google scholar: lookup
      2. KUCHLER RJ, MERCHANT DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures.. Proc Soc Exp Biol Med 1956 Aug-Sep;92(4):803-6.
        pubmed: 13370534doi: 10.3181/00379727-92-22620google scholar: lookup
      3. BRYANS JT, CROWE ME, DOLL ER, MCCOLLUM WH. Isolation of a filterable agent causing arteritis of horses and abortion by mares; its differentiation from the equine abortion (influenza) virus.. Cornell Vet 1957 Jan;47(1):3-41.
        pubmed: 13397177
      4. EPSTEIN MA. Observations on the mode of release of herpes virus from infected HeLa cells.. J Cell Biol 1962 Mar;12(3):589-97.
        pubmed: 13890436doi: 10.1083/jcb.12.3.589google scholar: lookup
      5. RANDALL CC, LAWSON LA. Adaptation of equine abortion virus to Earle's L cells in serum-free medium with plaque formation.. Proc Soc Exp Biol Med 1962 Jul;110:487-9.
        pubmed: 14490220doi: 10.3181/00379727-110-27558google scholar: lookup
      6. PLUMMER G, WATERSON AP. Equine herpes viruses.. Virology 1963 Mar;19:412-6.
        pubmed: 13944111doi: 10.1016/0042-6822(63)90083-7google scholar: lookup
      7. REYNOLDS ES. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.. J Cell Biol 1963 Apr;17(1):208-12.
        pubmed: 13986422doi: 10.1083/jcb.17.1.208google scholar: lookup
      8. ARHELGER RB, DARLINGTON RW, RANDALL CC. An electron microscopic study of equine abortion virus infection in hamster liver.. Am J Pathol 1963 Jun;42(6):703-13.
        pubmed: 14013743
      9. DARLINGTON RW, RANDALL CC. The nucleic acid content of equine abortion virus.. Virology 1963 Mar;19:322-7.
        pubmed: 14025123doi: 10.1016/0042-6822(63)90071-0google scholar: lookup
      10. HOLMES IH, WATSON DH. AN ELECTRON MICROSCOPE STUDY OF THE ATTACHMENT AND PENETRATION OF HERPES VIRUS IN BHK21 CELLS.. Virology 1963 Sep;21:112-23.
        pubmed: 14062901doi: 10.1016/0042-6822(63)90309-xgoogle scholar: lookup
      11. RECZKO E, MAYR A. [ON THE FINE STRUCTURE OF A VIRUS OF THE HERPES GROUP ISOLATED FROM HORSES (SHORT REPORT)].. Arch Gesamte Virusforsch 1963 Aug 26;13:591-3.
        pubmed: 14078849
      12. MELNICK JL, MIDULLA M, WIMBERLY I, BARRERA-ORO JG, LEVY BM. A NEW MEMBER OF THE HERPESVIRUS GROUP ISOLATED FROM SOUTH AMERICAN MARMOSETS.. J Immunol 1964 Apr;92:596-601.
        pubmed: 14139031
      13. PLUMMER G. SEROLOGICAL COMPARISON OF THE HERPES VIRUSES.. Br J Exp Pathol 1964 Apr;45(2):135-41.
        pubmed: 14144526
      14. ERLANDSON RA. A NEW MAGAGLAS, D.E.R.(R) 732, EMBEDMENT FOR ELECTRON MICROSCOPY.. J Cell Biol 1964 Sep;22(3):704-9.
        pubmed: 14206432doi: 10.1083/jcb.22.3.704google scholar: lookup
      15. STOKER M, MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.. Nature 1964 Sep 26;203:1355-7.
        pubmed: 14207308doi: 10.1038/2031355a0google scholar: lookup
      16. SOEHNER RL, GENTRY GA, RANDALL CC. SOME PHYSICOCHEMICAL CHARACTERISTICS OF EQUINE ABORTION VIRUS NUCLEIC ACID.. Virology 1965 Jul;26:394-405.
        pubmed: 14319712doi: 10.1016/0042-6822(65)90003-6google scholar: lookup
      17. EAGLE H. Nutrition needs of mammalian cells in tissue culture.. Science 1955 Sep 16;122(3168):501-14.
        pubmed: 13255879doi: 10.1126/science.122.3168.501google scholar: lookup
      18. PALADE GE. Studies on the endoplasmic reticulum. II. Simple dispositions in cells in situ.. J Biophys Biochem Cytol 1955 Nov 25;1(6):567-82.
        pubmed: 13278367doi: 10.1083/jcb.1.6.567google scholar: lookup
      19. MORGAN C, ROSE HM, HOLDEN M, JONES EP. Electron microscopic observations on the development of herpes simplex virus.. J Exp Med 1959 Oct 1;110(4):643-56.
        pubmed: 14424096doi: 10.1084/jem.110.4.643google scholar: lookup
      20. BECKER P, MELNICK JL, MAYOR HD. A MORPHOLOGIC COMPARISON BETWEEN THE DEVELOPMENTAL STAGES OF HERPES ZOSTER AND HUMAN CYTOMEGALOVIRUS.. Exp Mol Pathol 1965 Feb;4:11-23.
        pubmed: 14297547doi: 10.1016/0014-4800(65)90020-1google scholar: lookup

      Citations

      This article has been cited 10 times.
      1. Schwartz J, Roizman B. Similarities and Differences in the Development of Laboratory Strains and Freshly Isolated Strains of Herpes Simplex Virus in HEp-2 Cells: Electron Microscopy.. J Virol 1969 Dec;4(6):879-89.
        doi: 10.1128/JVI.4.6.879-889.1969pubmed: 16789121google scholar: lookup
      2. de Bruyn Kops A, Knipe DM. Preexisting nuclear architecture defines the intranuclear location of herpesvirus DNA replication structures.. J Virol 1994 Jun;68(6):3512-26.
        doi: 10.1128/JVI.68.6.3512-3526.1994pubmed: 8189490google scholar: lookup
      3. Stackpole CW. Herpes-type virus of the frog renal adenocarcinoma. I. Virus development in tumor transplants maintained at low temperature.. J Virol 1969 Jul;4(1):75-93.
        doi: 10.1128/JVI.4.1.75-93.1969pubmed: 5808113google scholar: lookup
      4. Moss LH 3rd, Gravell M. Ultrastructure and sequential development of infectious pancreatic necrosis virus.. J Virol 1969 Jan;3(1):52-8.
        doi: 10.1128/JVI.3.1.52-58.1969pubmed: 5772492google scholar: lookup
      5. Karpas A. The growth cycle of equine herpes 3 virus as compared with other viruses of the herpes group.. Arch Gesamte Virusforsch 1967;22(3):316-23.
        doi: 10.1007/BF01242952pubmed: 5628707google scholar: lookup
      6. Darlington RW, Moss LH 3rd. Herpesvirus envelopment.. J Virol 1968 Jan;2(1):48-55.
        doi: 10.1128/JVI.2.1.48-55.1968pubmed: 4316013google scholar: lookup
      7. O'Callaghan DJ, Hyde JM, Gentry GA, Randall CC. Kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells.. J Virol 1968 Aug;2(8):793-804.
        doi: 10.1128/JVI.2.8.793-804.1968pubmed: 4302745google scholar: lookup
      8. Nii S, Morgan C, Rose HM. Electron microscopy of herpes simplex virus. II. Sequence of development.. J Virol 1968 May;2(5):517-36.
        doi: 10.1128/JVI.2.5.517-536.1968pubmed: 4301317google scholar: lookup
      9. Lawrence WC. Evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the S phase of the KB cell mitotic cycle.. J Virol 1971 Jun;7(6):736-48.
        doi: 10.1128/JVI.7.6.736-748.1971pubmed: 4254680google scholar: lookup
      10. Abodeely RA, Lawson LA, Randall CC. Morphology and entry of enveloped and deenveloped equine abortion (herpes) virus.. J Virol 1970 Apr;5(4):513-23.
        doi: 10.1128/JVI.5.4.513-523.1970pubmed: 4195054google scholar: lookup
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