Biophysical and 1H magnetic resonance spectroscopy characteristics of fractionated stallion ejaculates.
Abstract: The composition of seminal plasma must be determined to assess the possible roles of sex gland secretions in survival of stallion spermatozoa. In the present study, an automated semen collection device and 1H magnetic resonance spectroscopy were used to analyse and compare the composition of seminal plasma from fractionated and nonfractionated stallion ejaculates. The contribution of each semen component to the ejaculate (sequence of production of component and concentration) was evaluated and its relationship to biophysical parameters was determined. 1H magnetic resonance spectroscopy was used to quantify molecules defined as markers of sex gland secretions: carnitine, glycerophosphorylcholine and choline for the epididymides; N-acetyl function of glycoproteins and spermine for the ampullae; acetic acid for the bulbourethral glands; and citric acid for seminal vesicles. The results from 32 ejaculates (four ejaculates from each of four stallions by two collection methods) demonstrated the reliability of the 1H magnetic resonance spectroscopy quantitation, the sequence of sex gland secretion contributions to the ejaculate (bulbourethral glands, epididymides, ampullae and seminal vesicles) and the concomitant appearance of the sperm-rich fraction with secretions from the epididymides and ampullae.
Publication Date: 2000-01-01 PubMed ID: 20681121
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study examines the composition of the seminal plasma in horse ejaculates and the role different secretions play in sperm survival, using an automated semen collection device and 1H magnetic resonance spectroscopy for accurate analysis.
Research Objectives
- The researchers aimed to determine the composition of the seminal plasma in stallion ejaculates. This is important in understanding the roles various sex gland secretions play in the survival of spermatozoa (sperm cells).
- To achieve this, an automated semen collection device was used alongside 1H magnetic resonance spectroscopy. These tools allowed for precise measurement and comparison of seminal plasma composition from fractionated (divided into fractions for separate analysis) and non-fractionated stallion ejaculates.
Methodology
- The study evaluated the contribution of each semen component to the ejaculate. This analysis involved understanding the sequence of production of each component and their respective concentrations. It also aimed to understand how these factors relate to biophysical parameters.
- The 1H magnetic resonance spectroscopy was used to quantify specific molecules known as markers of sex gland secretions. The markers included various biochemical compounds produced in different parts of the male reproductive system: carnitine, glycerophosphorylcholine and choline for the epididymides; N-acetyl function of glycoproteins and spermine for the ampullae; acetic acid for the bulbourethral glands; and citric acid for seminal vesicles.
Findings
- The results came from 32 ejaculates (four ejaculates each from four different stallions using two collection methods).
- The findings confirmed the reliability of 1H magnetic resonance spectroscopy for quantifying the sex gland secretion molecules in the seminal plasma.
- They established the sequence of sex gland secretion contributions to the ejaculate, in order from the bulbourethral glands, the epididymides, ampullae, and finally the seminal vesicles.
- Moreover, the researchers found a simultaneous appearance of the sperm-rich fraction along with secretions from the epididymides and ampullae.
Cite This Article
APA
Magistrini M, Lindeberg H, Koskinen E, Beau P, Seguin F.
(2000).
Biophysical and 1H magnetic resonance spectroscopy characteristics of fractionated stallion ejaculates.
J Reprod Fertil Suppl(56), 101-110.
Publication
Researcher Affiliations
- Equipe Reproduction Equine, INRA-Haras Nationaux, Station PRMD, 37380 Nouzilly, France.
MeSH Terms
- Animals
- Ejaculation / physiology
- Horses / physiology
- Magnetic Resonance Spectroscopy / methods
- Male
- Semen / chemistry
- Semen / physiology
- Specimen Handling / veterinary
Citations
This article has been cited 3 times.- Bazzano M, Zhu C, Laus F, Giambattista AD, Laghi L. Exploring the metabolome of seminal plasma in two different horse types: Light versus draft stallions. Reprod Domest Anim 2023 Jan;58(1):109-116.
- Tirpák F, Halo M Jr, Tokárová K, Binkowski LJ, Vašíček J, Svoradová A, Błaszczyk-Altman M, Kováčik A, Tvrdá E, Chrenek P, Lukáč N, Massányi P. Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality. Life (Basel) 2021 Nov 16;11(11).
- Castiglione Morelli MA, Ostuni A, Giangaspero B, Cecchini S, Carluccio A, Boni R. Relationships between Seminal Plasma Metabolites, Semen Characteristics and Sperm Kinetics in Donkey (Equus asinus). Animals (Basel) 2021 Jan 15;11(1).
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