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Journal of equine veterinary science2019; 84; 102837; doi: 10.1016/j.jevs.2019.102837

Blood Response to Mercury Exposure in Athletic Horse From Messina, Italy.

Abstract: Mercury (Hg) exists in various chemical forms, and it is different to health effects. The most toxic effects occur in the central nervous system during fetal development with irreversible alterations of the nerve cells of the cerebral cortex and, it is essential to monitor the tissue residues of Hg to protect the animal's health. The present investigation was carried out during May 2019 in 20 horses located in equine sport center near the area of Milazzo, Messina, that is a part of Sicilian territory heavily polluted by many toxic substances. The purpose of this study was to determine the concentration of Hg in blood, serum, food, and water administered to horses and from the hematological profile as a biomarker of blood in relation to the bioaccumulation of Hg. The hematologic parameters (red blood cell, white blood cell, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelets) were processed with an automated hematology analyzer, and Hg concentrations were determined by DMA-80. To carry out the variation in the Hg levels in serum and blood, the data were subjected to paired t-test analysis; post-hoc comparisons were performed using Tukey's test. The differences were considered statistically significant when P < .05. Paired t-test showed that in blood samples, Hg concentrations were significantly higher than the serum, and blood samples were positively correlated with serum (r = 0.99; P < .0001). The Hg contents determined in blood, serum, hay, and water samples were below the respective benchmarks, and therefore, we can exclude any toxicological risk for athletic horse.
Publication Date: 2019-11-09 PubMed ID: 31864458DOI: 10.1016/j.jevs.2019.102837Google Scholar: Lookup
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  • Journal Article

Summary

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This study investigated the levels of Mercury (Hg) exposure on athletic horses in Messina, Italy, a region known for high levels of toxic pollution. The research showed that the Mercury concentrations found in the horses’ blood, serum, food, and water were below dangerous levels, suggesting that the horses are not at a toxicological risk.

Research Methodology

  • The study was conducted in May 2019 on 20 horses from a sporting center near an area in Messina, heavily polluted with various toxic substances.
  • The aim was to determine the concentration of Mercury in the horses’ blood, serum, food, and water, and to study the effect of the bioaccumulation of Mercury on the blood biomarker.
  • The hematological parameters including red and white blood cell counts, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet count were taken into consideration.
  • An automated hematology analyzer was used to process the hematologic parameters, and Mercury concentrations were determined by DMA-80.
  • The variation in Mercury levels in serum and blood samples were subjected to paired t-test analysis, with post-hoc comparisons performed using Tukey’s test.

Research Findings

  • The results showed that in blood samples, Mercury concentrations were significantly higher than in the serum. However, the levels of Mercury in the blood were positively correlated with the serum, indicating that the serum could be used as a reliable indicator of blood Mercury levels.
  • The Mercury concentrations found in blood, serum, food, and water samples were below the reference benchmarks. This suggests that despite living in an area recognized for toxic pollution, the athletic horses studied are not at risk of any potential Mercury-related health issues.
  • The study confirmed that Mercury can have the most toxic effects on the central nervous system, particularly during fetal development, hence the importance of this work to ensure the ongoing health and safety of the sporting horses.

Conclusion

  • This research provides valuable information for authorities in polluted areas, as it suggests that despite the presence of harmful toxins such as Mercury in the environment, safety measures are, in this case, effectively protecting the health of the horses.
  • It also suggests that regular monitoring and testing of Mercury levels in animals’ blood and serum can be a useful bioindicator in maintaining their health and the wider environmental safety.

Cite This Article

APA
Fazio F, Cicero N, Piccione G, Giannetto C, Licata P. (2019). Blood Response to Mercury Exposure in Athletic Horse From Messina, Italy. J Equine Vet Sci, 84, 102837. https://doi.org/10.1016/j.jevs.2019.102837

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 84
Pages: 102837
PII: S0737-0806(19)30586-6

Researcher Affiliations

Fazio, Francesco
  • Department of Veterinary Science, University of Messina, Messina, Italy. Electronic address: ffazio@unime.it.
Cicero, Nicola
  • BioMorf Department, University of Messina, Messina, Italy.
Piccione, Giuseppe
  • Department of Veterinary Science, University of Messina, Messina, Italy.
Giannetto, Claudia
  • Department of Veterinary Science, University of Messina, Messina, Italy.
Licata, Patrizia
  • Department of Veterinary Science, University of Messina, Messina, Italy.

MeSH Terms

  • Animals
  • Erythrocyte Indices / veterinary
  • Hematocrit / veterinary
  • Horses
  • Italy
  • Mercury / analysis
  • Sports

Citations

This article has been cited 3 times.
  1. Fazio F, Gugliandolo E, Nava V, Piccione G, Giannetto C, Licata P. Bioaccumulation of Mineral Elements in Different Biological Substrates of Athletic Horse from Messina, Italy. Animals (Basel) 2020 Oct 14;10(10).
    doi: 10.3390/ani10101877pubmed: 33066668google scholar: lookup
  2. Popescu M, Tripon MA, Lupșan AF, Bungărdean D, Crecan CM, Musteata M, Pașca PM, Mârza SM, Purdoiu RC, Papuc I, Lăcătuș R, Lăcătuș CM, Panait LC, Patrichi TS, Matei IR, Sisea CR, Bunea CI, Călugăr A, Petrescu-Mag IV, Daradics Z, Bora FD. Sentinel Equines in Anthropogenic Landscapes: Bioaccumulation of Heavy Metals and Hematological Biomarkers as Indicators of Environmental Contamination. Toxics 2025 Dec 9;13(12).
    doi: 10.3390/toxics13121064pubmed: 41441284google scholar: lookup
  3. Acosta-Dacal A, Melián Henríquez A, Corbera JA, Macías-Montes A, Zumbado M, Ruiz-Suárez N, Martín-Barrasa JL, Luzardo OP, Tejedor-Junco MT. Comprehensive Profiling of Essential Elements and Organic and Inorganic Contaminants in Dromedary Camels from the Canary Islands: A Baseline for Nutritional and Environmental Assessment. Vet Sci 2025 Aug 29;12(9).
    doi: 10.3390/vetsci12090829pubmed: 41012755google scholar: lookup