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Acta biomaterialia2014; 10(9); 3969-3977; doi: 10.1016/j.actbio.2014.05.027

Bovine and equine peritubular and intertubular dentin.

Abstract: Dentin contains 1-2μm diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (∼200nm)(2), 10.1keV synchrotron X-ray beam maps X-ray fluorescence and X-ray diffraction simultaneously around tubules in 15-30μm thick bovine and equine specimens. Increased Ca fluorescence surrounding tubule lumens confirms that PTD is present, and the relative intensities in PTD and ITD correspond to carbonated apatite (cAp) volume fraction of ∼0.8 in PTD vs. 0.65 assumed for ITD. In the PTD near the lumen edges, Zn intensity is strongly peaked, corresponding to a Zn content of ∼0.9mgg(-1) for an assumed concentration of ∼0.4mgg(-1) for ITD. In the equine specimen, the Zn K-edge position indicates that Zn(2+) is present, similar to bovine dentin (Deymier-Black et al., 2013), and the above edge structure is consistent with spectra from macromolecules related to biomineralization. Transmission X-ray diffraction shows only cAp, and the 00.2 diffraction peak (Miller-Bravais indices) width is constant from ITD to the lumen edge. The cAp 00.2 average preferred orientation is axisymmetric (about the tubule axis) in both bovine and equine dentin, and the axisymmetric preferred orientation continues from ITD through the PTD to the tubule lumen. These data indicate that cAp structure does not vary from PTD to ITD.
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Publication Date: 2014-06-06 PubMed ID: 24911530PubMed Central: PMC4123743DOI: 10.1016/j.actbio.2014.05.027Google Scholar: Lookup
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Summary

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This research article examines and compares the compositions and structures of peritubular and intertubular dentin in bovine and equine specimens. The study uses advanced X-ray technologies to confirm the presence of key substances and structures, with findings indicating that the structure of carbonated apatite, a significant mineral component, does not vary between peritubular and intertubular dentin.

Dentin and the Study Focus

  • Dentin is a crucial component of teeth, containing microscopic tubules that extend from the center of the tooth towards the enamel junction.
  • There are two types of dentin: Peritubular Dentin (PTD), which envelops the tubule lumens, and Intertubular Dentin (ITD) that surrounds the PTD. However, differences in their microstructure and composition are not fully understood, prompting the need for this study.
  • The research aims to gain insights into these differences by analyzing bovine and equine dentin specimens using synchrotron X-ray beams.

Research Method and Findings

  • The high-energy X-ray beams mapped X-ray fluorescence and diffraction simultaneously, providing detailed insights into the structure and composition of the specimens.
  • Increased Calcium (Ca) fluorescence around the tubule lumen confirms the presence of PTD. Relative intensities suggest a carbonated apatite (cAp) volume fraction of approximately 0.8 in PTD versus 0.65 in ITD. cAp is a crucial mineral constituent of dentin.
  • Single-crystal Zn, with an intensity peak near the lumen edges in PTD, corresponds to a Zinc (Zn) content of about 0.9mgg(-1) assuming a concentration of 0.4mgg(-1) in ITD.

Results on Equine Specimen and Final Conclusions

  • In the equine specimen, Zn(2+) is present, and its edge structure aligns with spectra from biomolecules related to biomineralization.
  • Transmission X-ray diffraction shows only cAp and the cAp 00.2 average preferred orientation is the same in bovine and equine dentin and doesn’t change from ITD through the PTD to the tubule lumen.
  • These findings suggest that the cAp structure, a significant mineral element of dentin, remains constant from PTD to ITD.

Cite This Article

APA
Stock SR, Deymier-Black AC, Veis A, Telser A, Lux E, Cai Z. (2014). Bovine and equine peritubular and intertubular dentin. Acta Biomater, 10(9), 3969-3977. https://doi.org/10.1016/j.actbio.2014.05.027

Publication

Acta biomaterialia
ISSN: 1878-7568
NlmUniqueID: 101233144
Country: England
Language: English
Volume: 10
Issue: 9
Pages: 3969-3977
PII: S1742-7061(14)00239-6

Researcher Affiliations

Stock, S R
  • Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, 303 E. Chicago Ave, Chicago, IL 60611-3008, USA. Electronic address: s-stock@northwestern.edu.
Deymier-Black, A C
  • Department of Cell and Molecular Biology, Northwestern University, 303 E. Chicago Ave, Chicago, IL 60611-3008, USA.
Veis, A
  • Department of Cell and Molecular Biology, Northwestern University, 303 E. Chicago Ave, Chicago, IL 60611-3008, USA.
Telser, A
  • Department of Cell and Molecular Biology, Northwestern University, 303 E. Chicago Ave, Chicago, IL 60611-3008, USA.
Lux, E
  • Department of Cell and Molecular Biology, Northwestern University, 303 E. Chicago Ave, Chicago, IL 60611-3008, USA.
Cai, Z
  • Advanced Photon Source, Argonne National Laboratory, 9700 S. Cass Ave, Argonne, IL 60439, USA.

MeSH Terms

  • Animals
  • Calcium / analysis
  • Cattle
  • Crystallography, X-Ray
  • Dentin / chemistry
  • Dentin / diagnostic imaging
  • Fluorescence
  • Horses
  • Radiography
  • X-Ray Absorption Spectroscopy
  • Zinc / analysis

Grant Funding

  • R01 DE001374 / NIDCR NIH HHS
  • DE001374 / NIDCR NIH HHS

Conflict of Interest Statement

. The authors have no conflict of interest to report.

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Citations

This article has been cited 10 times.
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