Bovine papillomaviral gene expression in equine sarcoid tumours.
Abstract: The sarcoid is a benign locally invasive dermal fibroblastic lesion, commonly affecting horses and donkeys. The aetiology of the equine sarcoid is equivocal. Bovine papillomaviral (BPV) DNA (type 1/2) is frequently demonstrable in equine sarcoid tumour biopsies. However, the exact role of the virus in the disease process and its contribution to the phenotypic differences in sarcoids is not known. It was sought to assess the transcriptional activity of BPV-1 found in sarcoid tissues. Of 20 tumours examined, 18 were positive for E2 expression and ten positive for L1 expression. Viral oncogenes E5, E6 and E7 transcripts were detected in 16, nine and 12 tumours, respectively. This study demonstrates BPV gene expression in equine sarcoids and provide the first evidence for a direct involvement of the virus in the pathogenesis of sarcoids.
Publication Date: 1999-09-04 PubMed ID: 10475087DOI: 10.1016/s0168-1702(99)00022-2Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research discusses the presence and expression of Bovine papillomavirus (BPV) genes in equine sarcoid tumors, suggesting a role of this virus in the development of these tumors in horses and donkeys.
Introduction to the Study
- The research focuses on equine sarcoid tumors, a common benign skin tumor found among horses and donkeys.
- Researchers are not certain about what causes these tumors, but BPV DNA (type 1/2) is frequently found in biopsy samples of these tumors.
- The researchers aimed to determine the exact role the virus plays in the disease process and its contribution to the differences in the phenotype of sarcoids.
Methodology and Findings
- The researchers assessed the transcriptional activity of BPV-1—the process where the virus’s DNA is converted into RNA—in sarcoid tissues.
- Out of 20 tumors examined, 18 were found to express E2, a regulatory protein that controls the replication of BPV, and 10 expressed L1, a protein responsible for forming virus particles.
- The viral oncogenes E5, E6, and E7 were also detected in this study. Oncogenes are genes that can potentially cause cancer. Specifically, in 16, nine, and 12 tumors they detected E5, E6, and E7 transcripts respectively.
- This suggests these genes have been transcribed, indicating they are taking part in the disease process.
Significance of the Study
- This research provides proof of Bovine papillomavirus gene expression in the tissue of equine sarcoid tumors.
- This indicates a direct involvement of the virus in the pathogenesis—the development or progression—of these tumors, giving researchers valuable insight into the disease process.
- The findings of this study could help develop treatment strategies targeting the BPV genes.
Cite This Article
APA
Nasir L, Reid SW.
(1999).
Bovine papillomaviral gene expression in equine sarcoid tumours.
Virus Res, 61(2), 171-175.
https://doi.org/10.1016/s0168-1702(99)00022-2 Publication
Researcher Affiliations
- Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, UK.
MeSH Terms
- Animals
- Bovine papillomavirus 1
- Equidae / virology
- Horse Diseases / virology
- Horses / virology
- Papillomavirus Infections / veterinary
- Reverse Transcriptase Polymerase Chain Reaction
- Skin Neoplasms / veterinary
- Skin Neoplasms / virology
- Transcription, Genetic
- Tumor Virus Infections / veterinary
Citations
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