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Home / Equine Research Bank / Am J Vet Res / Bovine papillomavirus DNA in neoplastic and nonneoplastic ti...
American journal of veterinary research2001; 62(5); 741-744; doi: 10.2460/ajvr.2001.62.741

Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States.

Abstract: To determine the incidence of bovine papillomavirus (BPV) type 1 or 2 in sarcoids and other samples of cutaneous tissues collected from horses in the western United States. Methods: 55 horses with sarcoids and 12 horses without sarcoids. Methods: Tissue samples (tumor and normal skin from horses with sarcoids and normal skin, papillomas, and nonsarcoid cutaneous neoplasms from horses without sarcoids) were collected. Tissue samples were analyzed for BPV-1 or -2 DNA, using a polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products from 7 sarcoid-affected horses were sequenced to evaluate percentage homology with expected sequences for BPV-1 or-2. Results: Most (94/96, 98%) sarcoids contained BPV DNA. Sixty-two percent of the tumors examined had restriction enzyme patterns consistent with BPV-2. Thirty-one of 49 (63%) samples of normal skin obtained from horses with sarcoids contained BPV DNA. All samples subsequently sequenced had 100% homology with the expected sequences for the specific viral type. All tissues from healthy horses, nonsarcoid neoplasms, and papillomas were negative for BPV DNA. Conclusions: Bovine papillomaviral DNA was detected in essentially all sarcoids examined. There appears to be regional variation in the prevalence of viral types in these tumors. The fact that we detected viral DNA in normal skin samples from horses with sarcoids suggests the possibility of a latent viral phase. Viral latency may be 1 explanation for the high rate of recurrence following surgical excision of sarcoids.
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Publication Date: 2001-05-09 PubMed ID: 11341396DOI: 10.2460/ajvr.2001.62.741Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the prevalence of bovine papillomavirus (BPV) in samples of neoplastic and nonneoplastic tissues from horses in the western United States, particularly those with and without sarcoids, finding a high incidence of BPV in horses with the disease.

Objective and Methodology

  • The intention of this research was to determine the incidence of bovine papillomavirus type 1 or 2 in tumor and normal skin samples collected from a group of 55 horses with sarcoids and 12 horses without sarcoids.
  • The researchers utilized two common molecular testing techniques for this purpose: polymerase chain reaction (PCR) testing and restriction fragment length polymorphism. These methods allowed the scientists to detect and analyze the presence of BPV-1 or -2 DNA in the samples.
  • In addition, the PCR products from seven sarcoid affected horses also underwent sequencing to assess their homology to known BPV sequences.

Results

  • The researchers found that the vast majority of sarcoids (98%) contained BPV DNA, and around 62% of the tumors examined had restriction enzyme patterns consistent with BPV-2.
  • Further, 63% of normal skin samples from horses with sarcoids also contained BPV DNA.
  • All samples that underwent additional sequencing confirmed 100% homology with their respective expected BPV sequences.
  • Moreover, all tissue samples gathered from healthy horses and those with nonsarcoid neoplasms and papillomas were devoid of BPV DNA, underscoring the connection between BPV and sarcoids.

Conclusions

  • The research concluded that bovine papillomaviral DNA was present in nearly all sarcoids examined, indicating a strong association between the virus and the condition.
  • They also noted a regional variation in the prevalence of viral types in these tumors, which could suggest localized environmental or other influences on the types of BPV present.
  • The detection of viral DNA in normal skin samples from horses with sarcoids introduces the potential for a latent viral phase. This could explain the high rate of recurrence often seen following the surgical removal of sarcoids, as dormant viruses may reactivate and cause the disease to reemerge.

Cite This Article

APA
Carr EA, Théon AP, Madewell BR, Griffey SM, Hitchcock ME. (2001). Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States. Am J Vet Res, 62(5), 741-744. https://doi.org/10.2460/ajvr.2001.62.741

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 62
Issue: 5
Pages: 741-744

Researcher Affiliations

Carr, E A
  • Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
Théon, A P
    Madewell, B R
      Griffey, S M
        Hitchcock, M E

          MeSH Terms

          • Animals
          • Bovine papillomavirus 1 / genetics
          • Bovine papillomavirus 1 / growth & development
          • Cattle
          • DNA, Viral / genetics
          • DNA, Viral / isolation & purification
          • Histocytochemistry
          • Horse Diseases / pathology
          • Horse Diseases / virology
          • Horses
          • Papillomavirus Infections / pathology
          • Papillomavirus Infections / veterinary
          • Papillomavirus Infections / virology
          • Polymerase Chain Reaction / veterinary
          • Polymorphism, Restriction Fragment Length
          • Sarcoidosis / pathology
          • Sarcoidosis / veterinary
          • Sarcoidosis / virology
          • Skin Neoplasms / pathology
          • Skin Neoplasms / veterinary
          • Skin Neoplasms / virology
          • Tumor Virus Infections / pathology
          • Tumor Virus Infections / veterinary
          • Tumor Virus Infections / virology
          • United States

          Citations

          This article has been cited 20 times.
          1. Jindra C, Hainisch EK, Brandt S. Immunotherapy of Equine Sarcoids-From Early Approaches to Innovative Vaccines. Vaccines (Basel) 2023 Mar 30;11(4).
            doi: 10.3390/vaccines11040769pubmed: 37112681google scholar: lookup
          2. Hainisch EK, Jindra C, Reicher P, Miglinci L, Brodesser DM, Brandt S. Bovine Papillomavirus Type 1 or 2 Virion-Infected Primary Fibroblasts Constitute a Near-Natural Equine Sarcoid Model. Viruses 2022 Nov 28;14(12).
            doi: 10.3390/v14122658pubmed: 36560661google scholar: lookup
          3. Munday JS, Orbell G, Fairley RA, Hardcastle M, Vaatstra B. Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand Are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA Are Detectable in around 10% of Sarcoids. Animals (Basel) 2021 Oct 29;11(11).
            doi: 10.3390/ani11113093pubmed: 34827825google scholar: lookup
          4. Jindra C, Hainisch EK, Rümmele A, Wolschek M, Muster T, Brandt S. Influenza virus vector iNS1 expressing bovine papillomavirus 1 (BPV1) antigens efficiently induces tumour regression in equine sarcoid patients. PLoS One 2021;16(11):e0260155.
            doi: 10.1371/journal.pone.0260155pubmed: 34797850google scholar: lookup
          5. Munday JS, Gedye K, Daudt C, Chaves Da Silva F. The Development of Novel Primer Sets to Specifically Amplify Each of the Five Different Deltapapillomaviruses That Cause Neoplasia after Cross-Species Infection. Vet Sci 2021 Sep 26;8(10).
            doi: 10.3390/vetsci8100208pubmed: 34679038google scholar: lookup
          6. Jindra C, Kamjunke AK, Jones S, Brandt S. Screening for bovine papillomavirus type 13 (BPV13) in a European population of sarcoid-bearing equids. Equine Vet J 2021 Aug 30;54(4):662-9.
            doi: 10.1111/evj.13501pubmed: 34459020google scholar: lookup
          7. Savini F, Gallina L, Prosperi A, Puleio R, Lavazza A, Di Marco P, Tumino S, Moreno A, Lelli D, Guercio A, Scagliarini A. Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily. Pathogens 2020 May 30;9(6).
            doi: 10.3390/pathogens9060429pubmed: 32486181google scholar: lookup
          8. Savini F, Gallina L, Mazza F, Mariella J, Castagnetti C, Scagliarini A. Molecular Detection of Bovine Papillomavirus DNA in the Placenta and Blood of Healthy Mares and Respective Foals. Vet Sci 2019 Feb 6;6(1).
            doi: 10.3390/vetsci6010014pubmed: 30736349google scholar: lookup
          9. Bai DP, Lin XY, Huang YF, Zhang XF. Theranostics Aspects of Various Nanoparticles in Veterinary Medicine. Int J Mol Sci 2018 Oct 24;19(11).
            doi: 10.3390/ijms19113299pubmed: 30352960google scholar: lookup
          10. Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study. BMC Vet Res 2016 Feb 2;12:24.
            doi: 10.1186/s12917-016-0648-1pubmed: 26838095google scholar: lookup
          11. Wilford S, Woodward E, Dunkel B. Owners' perception of the efficacy of Newmarket bloodroot ointment in treating equine sarcoids. Can Vet J 2014 Jul;55(7):683-6.
            pubmed: 24982522
          12. Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids. J Clin Microbiol 2013 Jul;51(7):2167-71.
            doi: 10.1128/JCM.00371-13pubmed: 23637294google scholar: lookup
          13. Maglennon GA, Doorbar J. The biology of papillomavirus latency. Open Virol J 2012;6:190-7.
            doi: 10.2174/1874357901206010190pubmed: 23341854google scholar: lookup
          14. Corteggio A, Altamura G, Roperto F, Borzacchiello G. Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?. Infect Agent Cancer 2013 Jan 9;8(1):1.
            doi: 10.1186/1750-9378-8-1pubmed: 23302179google scholar: lookup
          15. Hainisch EK, Brandt S, Shafti-Keramat S, Van den Hoven R, Kirnbauer R. Safety and immunogenicity of BPV-1 L1 virus-like particles in a dose-escalation vaccination trial in horses. Equine Vet J 2012 Jan;44(1):107-11.
            doi: 10.1111/j.2042-3306.2011.00390.xpubmed: 21895749google scholar: lookup
          16. Wobeser BK, Davies JL, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL. Epidemiology of equine sarcoids in horses in western Canada. Can Vet J 2010 Oct;51(10):1103-8.
            pubmed: 21197201
          17. Reid N. Squamous cell carcinoma and suspect peripheral nerve sheath tumor in a 10-year-old Paint horse. Can Vet J 2009 Nov;50(11):1195-7.
            pubmed: 20119546
          18. Postey RC, Appleyard GD, Kidney BA. Evaluation of equine papillomas, aural plaques, and sarcoids for the presence of Equine papillomavirus DNA and Papillomavirus antigen. Can J Vet Res 2007 Jan;71(1):28-33.
            pubmed: 17193879
          19. Cutarelli A, Buonavoglia A, Fusco G, Pellicanò R, Napoletano M, Brandt S, Roperto S. Accurate identification of bovine deltapapillomavirus in equine sarcoids by ddPCR. Sci Rep 2025 Aug 11;15(1):29414.
            doi: 10.1038/s41598-025-15353-6pubmed: 40790360google scholar: lookup
          20. Smith CH, Stewart HL, Stefanovski D, Levine DG. Outcomes following autologous tumor tissue implantation with or without concurrent antineoplastic therapies in the treatment of sarcoids in 50 equids. Front Vet Sci 2025;12:1559519.
            doi: 10.3389/fvets.2025.1559519pubmed: 40417356google scholar: lookup
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