Calcium buffering is required to maintain bone stiffness in saline solution.
Abstract: This work determined whether mineral dissolution due to prolonged testing or storage of bone specimens in normal saline would alter their elastic modulus. In one experiment, small pieces of equine third metacarpal bone were soaked in normal saline supplemented with varying amounts of CaCl2. Changing Ca ion concentrations in the bath were monitored and the equilibrium concentration was determined. In a second experiment, the elastic moduli of twenty 4 x 10 x 100 mm equine third metacarpal beams were determined non-destructively in four-point bending. Half the beams were then soaked for 10 days in normal saline, and the other half in saline buffered to the bone mineral equilibrium point with Ca ions. Modulus measurements were repeated at 6 and 10 days. The equilibrium Ca ion concentration for bone specimens was found to be 57.5 mg l-1. The modulus of bone specimens soaked in normal saline significantly diminished 2.4%, whereas the modulus of those soaked in calcium-buffered saline did not change significantly.
Publication Date: 1996-09-01 PubMed ID: 8872276DOI: 10.1016/0021-9290(96)00020-6Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This research investigates how prolonged testing or storage of bone specimens in normal saline can lead to mineral dissolution, altering their elastic modulus. The results show that bones soaked in normal saline have a significant decrease in stiffness, but those soaked in calcium-buffered saline maintain their stiffness.
Objective
In this research, the scientists aim to understand if the elastic modulus, or stiffness, of bone specimens is affected by prolonged storage or testing in normal saline. They focus on equine third metacarpal bone pieces and beams to conduct their experiments.
Methodology
- The research was conducted in two separate experiments.
- In the first experiment, small pieces of equine third metacarpal bone were soaked in normal saline with various amounts of CaCl2 (calcium chloride).
- The changes in the concentrations of calcium ions in the soaking bath were monitored to determine equilibrium concentration.
- In the second experiment, the elastic moduli (measure of stiffness) of twenty equine third metacarpal bone pieces sized 4 x 10 x 100 mm were determined non-destructively through a process called four-point bending.
- Half of these bone beams were stored in normal saline for 10 days while the other half were placed in saline buffered with calcium ions to the bone mineral equilibrium point.
- Measurements of the moduli were repeated at the 6 and 10 day intervals.
Results
The findings from these experiments were as follows:
- The equilibrium concentration for the bone specimens was found to be 57.5 mg l-1.
- The stiffness of the bone specimens soaked in normal saline decreased significantly by 2.4%. This indicated the dissolution of minerals in the bones as a result of being stored in such a solution.
- Contrarily, the bones soaked in a saline solution buffered with calcium ions did not exhibit a significant change in stiffness. This suggested that the addition of calcium ions helped to mitigate the dissolution of bone minerals caused by soaking in normal saline.
Conclusion
From their findings, the researchers concluded that calcium buffering is therefore required to maintain the bone stiffness when bones are stored or tested in saline solution. Conversely, soaking bones in normal saline could lead to significant bone mineral dissolution, affecting their elastic modulus or inherent stiffness. This research provides an important contribution to the understanding of bone biophysics and has potential implications for bone health, particularly in relation to research or laboratory practices.
Cite This Article
APA
Gustafson MB, Martin RB, Gibson V, Storms DH, Stover SM, Gibeling J, Griffin L.
(1996).
Calcium buffering is required to maintain bone stiffness in saline solution.
J Biomech, 29(9), 1191-1194.
https://doi.org/10.1016/0021-9290(96)00020-6 Publication
Researcher Affiliations
- Orthopaedic Research Laboratories, School of Medicine, University of California at Davis, 95616, USA.
MeSH Terms
- Animals
- Bone and Bones / drug effects
- Bone and Bones / metabolism
- Bone and Bones / physiology
- Buffers
- Calcification, Physiologic
- Calcium / analysis
- Calcium / pharmacology
- Elasticity
- Female
- Horses
- Ions
- Male
- Osmolar Concentration
- Sodium Chloride / chemistry
- Sodium Chloride / pharmacology
- Time Factors
Grant Funding
- AR41644 / NIAMS NIH HHS
Citations
This article has been cited 20 times.- Bolger MW, Tekkey T, Kohn DH. The Contribution of Perilacunar Composition and Mechanical Properties to Whole-Bone Mechanical Outcomes in Streptozotocin-Induced Diabetes. Calcif Tissue Int 2023 Aug;113(2):229-245.
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