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Calcium/calmodulin and cAMP/protein kinase-A pathways regulate sperm motility in the stallion.
Abstract: In spite of the importance of sperm motility to fertility in the stallion, little is known about the signaling pathways that regulate motility in this species. In other mammals, calcium/calmodulin signaling and the cyclic AMP/protein kinase-A pathway are involved in sperm motility regulation. We hypothesized that these pathways also were involved in the regulation of sperm motility in the stallion. Using immunoblotting, calmodulin and the calmodulin-dependent protein kinase II β were shown to be present in stallion sperm and with indirect immunofluorescence calmodulin was localized to the acrosome and flagellar principal piece. Additionally, inhibition of either calmodulin or protein kinase-A significantly reduced sperm motility without affecting viability. Following inhibition of calmodulin, motility was not restored with agonists of the cyclic AMP/protein kinase-A pathway. These data suggest that calcium/calmodulin and cyclic AMP/protein kinase-A pathways are involved in the regulation of stallion sperm motility. The failure of cyclic AMP/protein kinase-A agonists to restore motility of calmodulin inhibited sperm suggests that both pathways may be required to support normal motility.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication Date: 2012-05-24 PubMed ID: 22687341DOI: 10.1016/j.anireprosci.2012.05.007Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- N.I.H.
- Extramural
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigated the role of calcium/calmodulin signaling and the cyclic AMP/protein kinase-A pathway in regulating sperm motility in stallions, contributing to a better understanding of equine fertility.
Research Purpose and Hypothesis
- The study aimed to enhance understanding of sperm motility regulation in stallions, an aspect crucial to fertility. Despite its importance, limited knowledge exists on the signaling pathways involved in stallion sperm motility regulation.
- The researchers hypothesized that calcium/calmodulin signaling and the cyclic AMP/protein kinase-A pathway, known to regulate sperm motility in other mammals, also play a role in stallions.
Research Methodology
- Calmodulin and calmodulin-dependent protein kinase II β in stallion sperm were identified using immunoblotting. Indirect immunofluorescence localized calmodulin to the acrosome and flagellar principal piece (parts of the sperm cell).
- The study used inhibitory substances on both pathways to measure their impact on sperm motility and viability. Any changes in these characteristics would suggest a role for these pathways in their regulation.
Research Findings
- The study confirmed the presence of calmodulin and its dependent kinase in stallion sperm and its location in the acrosome and flagellar principal piece.
- When either calmodulin or protein kinase-A were inhibited, the stallion sperm experienced a significant reduction in motility, while viability remained unaffected. This indicates a role for these two pathways in sperm motility regulation in stallions.
- The researchers further found that if motility was reduced through calmodulin inhibition, it could not be restored with cyclic AMP/protein kinase-A agonists. This suggests that both pathways may need to function simultaneously for normal sperm motility.
Research Conclusion
- Ultimately, the study supports the hypothesis that calcium/calmodulin and the cyclic AMP/protein kinase-A pathways play crucial roles in regulating stallion sperm motility.
- More so, it suggests that both pathways have to function together for normal sperm motility, thus contributing to the understanding of equine fertility regulation.
Cite This Article
APA
Lasko J, Schlingmann K, Klocke A, Mengel GA, Turner R.
(2012).
Calcium/calmodulin and cAMP/protein kinase-A pathways regulate sperm motility in the stallion.
Anim Reprod Sci, 132(3-4), 169-177.
https://doi.org/10.1016/j.anireprosci.2012.05.007 Publication
Researcher Affiliations
- Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, 382 West Street Rd., Kennett Square, PA 19348, USA.
MeSH Terms
- Animals
- Calcium / metabolism
- Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics
- Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism
- Calmodulin / antagonists & inhibitors
- Calmodulin / metabolism
- Cyclic AMP / metabolism
- Cyclic AMP-Dependent Protein Kinases / genetics
- Cyclic AMP-Dependent Protein Kinases / metabolism
- Gene Expression Regulation / physiology
- Horses / physiology
- Isoquinolines / pharmacology
- Male
- Sperm Motility / physiology
- Spermatozoa / enzymology
- Spermatozoa / physiology
- Sulfonamides / pharmacology
Citations
This article has been cited 8 times.- Sahoo B, Gupta MK. Effect of arginine-induced motility and capacitation on RNA population in goat spermatozoa. Vet Res Commun 2023 Sep;47(3):1427-1444.
- Iskandar H, Andersson G, Sonjaya H, Arifiantini RI, Said S, Hasbi H, Maulana T, Baharun A. Protein Identification of Seminal Plasma in Bali Bull (Bos javanicus). Animals (Basel) 2023 Feb 1;13(3).
- Maghsoumi-Norouzabad L, Zare Javid A, Mansoori A, Dadfar M, Serajian A. The effects of Vitamin D3 supplementation on Spermatogram and endocrine factors in asthenozoospermia infertile men: a randomized, triple blind, placebo-controlled clinical trial. Reprod Biol Endocrinol 2021 Jul 5;19(1):102.
- Romero-Aguirregomezcorta J, Sugrue E, Martínez-Fresneda L, Newport D, Fair S. Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species. Sci Rep 2018 Nov 15;8(1):16897.
- Kwon WS, Rahman MS, Lee JS, Yoon SJ, Park YJ, Pang MG. Discovery of predictive biomarkers for litter size in boar spermatozoa. Mol Cell Proteomics 2015 May;14(5):1230-40.
- Quattrone A, Fehri NE, Agradi S, Menchetti L, Barbato O, Castrica M, Sulçe M, Castellini C, Muça G, Mattioli S, Vigo D, Migni G, Nompleggio L, Belabbas R, Gualazzi F, Ricci G, Postoli R, Di Federico F, Moretti E, Zalla P, Collodel G, Brecchia G, Curone G. In Vitro Effects of Lipopolysaccharide on Rabbit Sperm: Toll-like Receptor 4 Expression, Motility, and Oxidative Status. Antioxidants (Basel) 2025 Apr 2;14(4).
- Maulana T, Said S, Arifiantini RI, Jakaria J, Gunawan A. The frozen-thawed sperm protein of Indonesian Toraya buffaloes is significantly associated with sperm kinematics, acrosome integrity, and mitochondrial membrane potential. J Adv Vet Anim Res 2024 Dec;11(4):869-879.
- Khan MZ, Chen W, Naz S, Liu X, Liang H, Chen Y, Kou X, Liu Y, Ashraf I, Han Y, Peng Y, Wang C, Zahoor M. Determinant genetic markers of semen quality in livestock. Front Endocrinol (Lausanne) 2024;15:1456305.
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