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Home / Equine Research Bank / Equine Vet J / Cartilage oligomeric matrix protein and hyaluronan levels in...
Equine veterinary journal2006; 38(6); 502-507; doi: 10.2746/042516406x156073

Cartilage oligomeric matrix protein and hyaluronan levels in synovial fluid from horses with osteoarthritis of the tarsometatarsal joint compared to a control population.

Abstract: Quantification of cartilage oligomeric matrix protein (COMP) levels within synovial fluid from the tarsometatarsal joint has not previously been reported and an effective synovial fluid marker would allow monitoring of disease progression and treatment. Objective: To quantify levels of COMP and hyaluronan (HA) in synovial fluid from the tarsometatarsal joint, identify differences in levels from horses with osteoarthritis (OA) of the tarsometatarsal joint compared to a control population and to correlate levels with radiographic changes in horses with OA. Methods: Synovial fluid was collected from the tarsometatarsal joint of 25 horses without hindlimb lameness (controls) and 25 lame horses, subjected to analgesia of the joint. COMP concentrations were measured using a homologous inhibition ELISA. Immunoblots of synovial fluid from 3 lame horses and 3 controls were performed to identify fragmentation of COMP. Hyaluronan (HA) concentration in synovial fluid was determined using a competition ELISA. Radiographs of the lame horses with OA were scored and correlated with levels of COMP and HA. Results: Concentrations of COMP in OA of the tarsometatarsal joint were significantly lower than in the control samples. An additional fragment band of COMP (approximately 30 kDa) was identified on the immunoblots of the horses with OA and this fragment was not identified in controls. No significant difference was identified in the HA or HA:COMP ratio between lame and control horses. There was no correlation between levels of synovial fluid COMP and HA, and radiographic changes. Conclusions: Lowered levels of COMP in synovial fluid of tarsometatarsal joints correlates with the presence of osteoarthritis. However, a single value cannot be used to stage the disease process. Levels of HA may not be a useful marker for this disease. Decreased, rather than increased COMP levels, may reflect significant loss of cartilage in established osteoarthritis. A specific assay for the COMP fragment generated with osteoarthritis may allow the earlier detection of clinical cases.
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Publication Date: 2006-11-28 PubMed ID: 17124839DOI: 10.2746/042516406x156073Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The research investigates the levels of cartilage oligomeric matrix protein (COMP) and hyaluronan (HA) in the synovial fluid of horses suffering from osteoarthritis in the tarsometatarsal joint, comparing these levels to healthy, control horses in order to understand whether these could be useful indicators in monitoring disease progression and treatment.

Research Objective and Methods

  • The primary goal of this research was to quantify the levels of COMP and HA in the synovial fluid from the tarsometatarsal joint, and check for any significant differences in these levels between horses with osteoarthritis and a control population. It aimed to establish a correlation between these levels and any radiographic changes noticed in the horses suffering from osteoarthritis. To carry this out, synovial fluid was collected from the tarsometatarsal joint of 25 lame horses (with osteoarthritis), and 25 healthy horses.
  • The concentrations of COMP were measured using a homologous inhibition ELISA while hyaluronan concentration in the synovial fluid was determined using a competition ELISA.
  • Radiographic images of the afflicted horses were taken, scored, and then an attempt to correlate the scores with the levels of COMP and HA was conducted.

Results

  • The research found that COMP concentrations in the synovial fluid of horses with osteoarthritis were significantly lower than in the fluid from the control group.
  • The study also identified an additional fragment band of COMP (approximately 30 kDa) in the afflicted horses, which were not present in the controls.
  • However, the study observed no significant differences in HA or the HA:COMP ratio between the patient and control groups.
  • They found no correlation between the levels of COMP and HA in the synovial fluid, and the radiographic changes observed.

Conclusion

  • The results suggested that lowered COMP levels in the synovial fluid correlate with the presence of osteoarthritis, but these levels cannot be used singularly to establish the stage of the disease.
  • The HA levels may not serve as a useful marker for osteoarthritis.
  • A noteworthy conclusion drawn was that decrease, rather than increase, in COMP levels might reflect significant loss of cartilage in well-established osteoarthritis cases.
  • The researchers suggested future exploration of a specific assay for the COMP fragment correlated with osteoarthritis as it may enable earlier detection of clinical cases.

Cite This Article

APA
Taylor SE, Weaver MP, Pitsillides AA, Wheeler BT, Wheeler-Jones CP, Shaw DJ, Smith RK. (2006). Cartilage oligomeric matrix protein and hyaluronan levels in synovial fluid from horses with osteoarthritis of the tarsometatarsal joint compared to a control population. Equine Vet J, 38(6), 502-507. https://doi.org/10.2746/042516406x156073

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 38
Issue: 6
Pages: 502-507

Researcher Affiliations

Taylor, S E
  • Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK.
Weaver, M P
    Pitsillides, A A
      Wheeler, B T
        Wheeler-Jones, C P D
          Shaw, D J
            Smith, R K W

              MeSH Terms

              • Animals
              • Biomarkers / cerebrospinal fluid
              • Case-Control Studies
              • Disease Progression
              • Enzyme-Linked Immunosorbent Assay / methods
              • Enzyme-Linked Immunosorbent Assay / veterinary
              • Extracellular Matrix Proteins / cerebrospinal fluid
              • Female
              • Glycoproteins / cerebrospinal fluid
              • Horse Diseases / diagnosis
              • Horse Diseases / diagnostic imaging
              • Horse Diseases / pathology
              • Horses
              • Hyaluronic Acid / cerebrospinal fluid
              • Immunoblotting / methods
              • Immunoblotting / veterinary
              • Lameness, Animal / diagnosis
              • Lameness, Animal / diagnostic imaging
              • Lameness, Animal / pathology
              • Male
              • Matrilin Proteins
              • Osteoarthritis / diagnosis
              • Osteoarthritis / diagnostic imaging
              • Osteoarthritis / pathology
              • Osteoarthritis / veterinary
              • Radiography
              • Tarsal Joints / metabolism

              Citations

              This article has been cited 9 times.
              1. Laus F, Gialletti R, Bazzano M, Laghi L, Dini F, Marchegiani A. Synovial Fluid Metabolome Can Differentiate between Healthy Joints and Joints Affected by Osteoarthritis in Horses. Metabolites 2023 Aug 4;13(8).
                doi: 10.3390/metabo13080913pubmed: 37623857google scholar: lookup
              2. McParland TJ, Horne CR, Robertson JB, Schnabel LV, Nelson NC. Alterations to the synovial invaginations of the navicular bone are associated with pathology of both the navicular apparatus and distal interphalangeal joint when evaluated using high field MRI. Vet Radiol Ultrasound 2023 Jan;64(1):9-17.
                doi: 10.1111/vru.13140pubmed: 35969227google scholar: lookup
              3. Anderson JR, Phelan MM, Foddy L, Clegg PD, Peffers MJ. Ex Vivo Equine Cartilage Explant Osteoarthritis Model: A Metabolomics and Proteomics Study. J Proteome Res 2020 Sep 4;19(9):3652-3667.
                doi: 10.1021/acs.jproteome.0c00143pubmed: 32701294google scholar: lookup
              4. Smith R, Önnerfjord P, Holmgren K, di Grado S, Dudhia J. Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease. Int J Mol Sci 2020 Mar 20;21(6).
                doi: 10.3390/ijms21062155pubmed: 32245107google scholar: lookup
              5. Bourebaba L, Röcken M, Marycz K. Osteochondritis dissecans (OCD) in Horses - Molecular Background of its Pathogenesis and Perspectives for Progenitor Stem Cell Therapy. Stem Cell Rev Rep 2019 Jun;15(3):374-390.
                doi: 10.1007/s12015-019-09875-6pubmed: 30796679google scholar: lookup
              6. Anderson JR, Phelan MM, Clegg PD, Peffers MJ, Rubio-Martinez LM. Synovial Fluid Metabolites Differentiate between Septic and Nonseptic Joint Pathologies. J Proteome Res 2018 Aug 3;17(8):2735-2743.
                doi: 10.1021/acs.jproteome.8b00190pubmed: 29969035google scholar: lookup
              7. Bertuglia A, Pagliara E, Grego E, Ricci A, Brkljaca-Bottegaro N. Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career. BMC Vet Res 2016 Nov 8;12(1):246.
                doi: 10.1186/s12917-016-0873-7pubmed: 27821120google scholar: lookup
              8. Wanner J, Subbaiah R, Skomorovska-Prokvolit Y, Shishani Y, Boilard E, Mohan S, Gillespie R, Miyagi M, Gobezie R. Proteomic profiling and functional characterization of early and late shoulder osteoarthritis. Arthritis Res Ther 2013 Nov 6;15(6):R180.
                doi: 10.1186/ar4369pubmed: 24286485google scholar: lookup
              9. Anderson JR, Phelan MM, Caamaño-Gutiérrez E, Clegg PD, Rubio-Martinez LM, Peffers MJ. Metabolomic and proteomic stratification of equine osteoarthritis. Equine Vet J 2025 Sep;57(5):1204-1218.
                doi: 10.1111/evj.14490pubmed: 39972657google scholar: lookup
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