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Home / Equine Research Bank / Vet Res / CCL2 and CCL5 driven attraction of CD172a+ monocytic cells d...
Veterinary research2017; 48(1); 14; doi: 10.1186/s13567-017-0419-4

CCL2 and CCL5 driven attraction of CD172a+ monocytic cells during an equine herpesvirus type 1 (EHV-1) infection in equine nasal mucosa and the impact of two migration inhibitors, rosiglitazone (RSG) and quinacrine (QC).

Abstract: Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Besides epithelial cells, CD172a monocytic cells become infected with EHV-1 in the respiratory mucosa and transport the virus from the apical side of the epithelium to the lamina propria en route to the lymph and blood circulation. Whether CD172a monocytic cells are specifically recruited to the infection sites in order to pick up virus is unknown. In our study, equine nasal mucosa explants were inoculated with EHV-1 neurological strains 03P37 and 95P105 or the non-neurological strains 97P70 and 94P247 and the migration of monocytic cells was examined by immunofluorescence. Further, the role of monokines CCL2 and CCL5 was determined and the effect of migration inhibitors rosiglitazone (RSG) or quinacrine was analyzed. It was shown that with neurological strains but not with the non-neurological strains, CD172a cells specifically migrated towards EHV-1 infected regions and that CCL2 and CCL5 were involved. CCL2 started to be expressed in infected epithelial cells at 24 h post-incubation (hpi) and CCL5 at 48 hpi, which corresponded with the CD172a migration. RSG treatment of EHV-1-inoculated equine nasal mucosa had no effect on the virus replication in the epithelium, but decreased the migration of CD172a cells in the lamina propria. Overall, these findings bring new insights in the early pathogenesis of EHV-1 infections, illustrate differences between neurological and non-neurological strains and show the way for EHV-1 treatment.
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Publication Date: 2017-02-27 PubMed ID: 28241864PubMed Central: PMC5327560DOI: 10.1186/s13567-017-0419-4Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focused on understanding how a type of horse herpes virus (EHV-1) interacts with certain cells in the horse’s respiratory system and how two drugs might affect this interaction. The researchers observed differences between neurological and non-neurological strains of the virus and suggest their findings may provide new insights for treating EHV-1 infections.

Research Objective and Methodology

  • The researchers aimed to understand whether specific monocytic cells, known as CD172a cells, are purposely attracted to infection areas to collect the EHV-1 virus in horses.
  • Equine nasal mucosa explants were infected with both neurological (03P37 and 95P105) and non-neurological (97P70 and 94P247) strains of EHV-1. The researchers then examined the migration patterns of CD172a cells.
  • In addition, they evaluated the roles of monokines CCL2 and CCL5, two substances assumed to play a role in cell migration. They also examined how migration inhibitors rosiglitazone (RSG) and quinacrine (QC) influence these processes.

Findings and Interpretations

  • With neurological strains of EHV-1, but not the non-neurological strains, CD172a cells migrated towards EHV-1 infected areas. This suggests a selectively reactive response from CD172a cells towards distinct EHV-1 strains.
  • Monokines CCL2 and CCL5 were implicated in this process. CCL2 began to be expressed in infected epithelial cells 24 hours after exposure to the virus, and CCL5 at 48 hours. This time frame aligns with when the CD172a cell migration occurred, suggesting a relationship between them.
  • RSG, when introduced to the EHV-1 infected equine nasal mucosa, did not influence virus replication in the epithelium. However, the application of RSG did reduce the migration of CD172a cells in the part of the mucosa underneath the epithelium.

Conclusion and Potential Implications

  • The study’s findings provided new information about the early stages of EHV-1 infections, demonstrating differences between neurological and non-neurological virus strains.
  • The way CD172a cells interact with the EHV-1 virus and how this interaction is influenced by CCL2 and CCL5 may influence future research on disease spread and containment strategies.
  • The observed effect of RSG on CD172a cell migration could have implications for developing treatments for EHV-1 infections, though it is important to note that the study did not find an effect of RSG on viral replication.

Cite This Article

APA
Zhao J, Poelaert KCK, Van Cleemput J, Nauwynck HJ. (2017). CCL2 and CCL5 driven attraction of CD172a+ monocytic cells during an equine herpesvirus type 1 (EHV-1) infection in equine nasal mucosa and the impact of two migration inhibitors, rosiglitazone (RSG) and quinacrine (QC). Vet Res, 48(1), 14. https://doi.org/10.1186/s13567-017-0419-4

Publication

Veterinary research
ISSN: 1297-9716
NlmUniqueID: 9309551
Country: England
Language: English
Volume: 48
Issue: 1
Pages: 14
PII: 14

Researcher Affiliations

Zhao, Jing
  • Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Poelaert, Katrien C K
  • Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Van Cleemput, Jolien
  • Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Nauwynck, Hans J
  • Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium. Hans.Nauwynck@UGent.be.

MeSH Terms

  • Animals
  • Cell Movement / drug effects
  • Chemokine CCL2 / physiology
  • Chemokine CCL5 / physiology
  • Fluorescent Antibody Technique / veterinary
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Equid
  • Horse Diseases / immunology
  • Horse Diseases / virology
  • Horses / virology
  • Monocytes / drug effects
  • Monocytes / physiology
  • Nasal Mucosa / immunology
  • Nasal Mucosa / virology
  • Quinacrine / pharmacology
  • Receptors, Immunologic / physiology
  • Rosiglitazone
  • Thiazolidinediones / pharmacology

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Citations

This article has been cited 5 times.
  1. Zarski LM, Vaala WE, Barnett DC, Bain FT, Soboll Hussey G. A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1.. Front Vet Sci 2021;8:674850.
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  2. Laval K, Poelaert KCK, Van Cleemput J, Zhao J, Vandekerckhove AP, Gryspeerdt AC, Garré B, van der Meulen K, Baghi HB, Dubale HN, Zarak I, Van Crombrugge E, Nauwynck HJ. The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1.. Front Microbiol 2021;12:662686.
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  4. Poelaert KCK, Van Cleemput J, Laval K, Favoreel HW, Couck L, Van den Broeck W, Azab W, Nauwynck HJ. Equine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Target Organs.. J Virol 2019 Apr 1;93(7).
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  5. Poelaert KCK, Van Cleemput J, Laval K, Favoreel HW, Soboll Hussey G, Maes RK, Nauwynck HJ. Abortigenic but Not Neurotropic Equine Herpes Virus 1 Modulates the Interferon Antiviral Defense.. Front Cell Infect Microbiol 2018;8:312.
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