CD47 expression in cryopreserved equine cutaneous masses and normal skin.
Abstract: We investigated CD47 expression in cryopreserved sections of equine cutaneous masses and normal skin. CD47 is a cell surface protein expressed on many cell types and overexpressed in some tumors. Interaction of CD47 and signal regulatory protein-alpha (SIRPα) inhibits phagocytosis by macrophages. Formalin-fixed tissues from horses prospectively enrolled in the study were used to establish a histologic diagnosis. Immunohistochemical assays were performed on cryopreserved tissues using anti-CD47 antibodies or IgG control antibodies. CD47 was not expressed on equine normal skin but positivity to CD47 was present in 13 of 24 (54%) masses. Immunotherapy with anti-CD47 antibodies for equine cutaneous tumors that express CD47 warrants further investigation.
© 2016 The Author(s).
Publication Date: 2016-05-06 PubMed ID: 27154320DOI: 10.1177/1040638716643352Google Scholar: Lookup
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- Journal Article
Summary
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The research article presents an investigation into the expression of CD47, a cell surface protein, in cryopreserved equine cutaneous masses and normal skin.
Objective of the Research
The study was designed to observe and evaluate the expression of the CD47 protein in preserved equine cutaneous masses and normal skin. The ultimate goal was to explore the potential development of immunotherapy for equine cutaneous tumors that express CD47.
CD47 Expression and its Importance
- The CD47 protein is found on the surface of various cell types, with some tumors exhibiting overexpression.
- Interaction between CD47 and SIRPα (signal regulatory protein-alpha) results in the inhibition of phagocytosis by macrophages, potentially enabling tumor cells to evade immune elimination.
- Investigating CD47’s expression could contribute to the understanding of its function in tumor formation and growth, which could inform potential therapeutic approaches.
Methodology
- Sample tissues were obtained from horses that were prospectively enrolled in this study and then fixed with formalin for histologic diagnosis.
- Immunohistochemical assays were performed on these cryopreserved tissues using antibodies against CD47 or IgG control antibodies.
Findings
- Findings showed that CD47 was not expressed on equine normal skin. However, of the 24 examined masses, 13 (54%) exhibited positivity to CD47.
- This indicates a significant occurrence of CD47 in cases of equine cutaneous tumors.
Implication and Future Direction
- A better understanding of CD47 expression in equine cutaneous tumors may pave the way for the design of innovative immunotherapies.
- Particularly, the use of anti-CD47 antibodies could potentially disable the “don’t eat me” signal provided by CD47, thus promoting the destruction of tumor cells by macrophages.
- This finding warrants further exploration and validation in expanded studies.
Cite This Article
APA
Caston SS, Cooper EE, Chandramani-Shivalingappa P, Sponseller BA, Hostetter JM, Sun Y.
(2016).
CD47 expression in cryopreserved equine cutaneous masses and normal skin.
J Vet Diagn Invest, 28(4), 408-413.
https://doi.org/10.1177/1040638716643352 Publication
Researcher Affiliations
- Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA scaston@iastate.edu.
- Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
- Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
- Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
- Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
- Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
MeSH Terms
- Animals
- CD47 Antigen / analysis
- Cryopreservation / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / pathology
- Horses
- Prospective Studies
- Skin Neoplasms / diagnosis
- Skin Neoplasms / pathology
- Skin Neoplasms / veterinary
Citations
This article has been cited 1 times.- Pimenta J, Prada J, Cotovio M. Equine Melanocytic Tumors: A Narrative Review. Animals (Basel) 2023 Jan 10;13(2).
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