Cellular and humoral defence mechanisms in mares susceptible and resistant to persistent endometritis.
Abstract: Both random and directional migration of blood neutrophils from 9 mares susceptible to persistent endometritis were significantly less (p less than 0.05) than neutrophils from 8 resistant mares. Serum from susceptible mares had significantly more (p less than 0.01) chemotactic activity than serum from resistant mares. Although phagocytosis of yeast blastospores by blood neutrophils from 4 resistant and 3 susceptible mares was similar, uterine neutrophils from susceptible mares were significantly worse (p less than 0.01) at phagocytosis than uterine neutrophils from resistant mares. Uterine washings from 17 susceptible mares were significantly better at opsonising yeast blastospores than washings from 14 resistant mares; however, washings from both groups had a similar ability to promote killing of S. zooepidemicus by neutrophils. When an immunologically non-specific endometritis was induced, washings from 3 susceptible mares were significantly worse at promoting bactericidal activity by 144 h than washings from 4 resistant mares (p less than 0.01). Haemolytic complement activity was significantly greater (p less than 0.001) in washings from 17 susceptible mares than from 14 resistant mares. Induction of acute endometritis resulted in high levels of haemolytic complement activity in 2 of 3 susceptible mares at 24 and 144 h, but only in small increases in 4 resistant mares. Thus, some abnormalities in neutrophil function were detected and a possible defect in promotion of neutrophil bactericidal activity by uterine secretions from susceptible mares but there was no evidence for any deficiency in haemolytic complement activity.
Publication Date: 1987-09-01 PubMed ID: 3314110DOI: 10.1016/0165-2427(87)90178-4Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article identifies differences in cellular and humoral defence mechanisms in mares prone to, and resistant against persistent endometritis. It notices significant variations in the performance of blood neutrophils, phagocytosis process, and haemolytic complement activity between the two groups, and links them to the progression or resistance of endometritis.
Neutrophil Migration
- The study examined the behavior of blood neutrophils, a type of white blood cell, in 9 susceptible and 8 resistant mares.
- Both random and planned movement of blood neutrophils in susceptible mares were significantly lesser than in resistant mares, hinting at a reduced immune response in the former.
Chemotactic Activity
- On testing the chemotactic activity, which is the movement of an organism or cell in response to a chemical stimulus, it was found that serum from susceptible mares had significantly more chemotactic activity than the serum from resistant mares.
Phagocytosis Process
- There was no significant difference in the phagocytosis, or the process by which cells ingest harmful particles, by blood neutrophils from both groups on exposure to yeast blastospores.
- However, neutrophils from the uterine lining of susceptible mares were significantly inferior at phagocytosis compared to those from resistant mares.
Opsonisation and Bactericidal Activity
- Uterine washings from susceptible mares were better at opsonising, or making bacteria more susceptible to phagocytosis, yeast blastospores than those from resistant mares.
- Although, both groups showed similar efficiency in killing the bacterium S. zooepidemicus with the help of neutrophils.
- However, when non-specific endometritis was induced, the bactericidal activity in susceptible mares declined faster compared to resistant mares.
Haemolytic Complement Activity
- Haemolytic complement activities, which are part of the immune system that aids cells in removing pathogens, were considerably higher in washings from susceptible mares than from resistant mares.
- Following the induction of acute endometritis, high levels of haemolytic complement activity were observed in susceptible mares, while minor increases were noted in resistant ones, indicating a possible overactive immune response in susceptible mares.
Conclusion
- The research detected certain abnormalities in neutrophil function and proposed a possible flaw in utilizing neutrophil bactericidal activity by uterine secretions in susceptible mares.
- However, it didn’t find any deficiency in the haemolytic complement activity among these mares.
Cite This Article
APA
Watson ED, Stokes CR, Bourne FJ.
(1987).
Cellular and humoral defence mechanisms in mares susceptible and resistant to persistent endometritis.
Vet Immunol Immunopathol, 16(1-2), 107-121.
https://doi.org/10.1016/0165-2427(87)90178-4 Publication
Researcher Affiliations
- Department of Veterinary Medicine, University of Bristol, Langford, England.
MeSH Terms
- Animals
- Chemotaxis, Leukocyte
- Complement System Proteins / metabolism
- Endometritis / etiology
- Endometritis / immunology
- Endometritis / veterinary
- Female
- Horse Diseases / etiology
- Horse Diseases / immunology
- Horses
- Immunity, Cellular
- In Vitro Techniques
- Neutrophils / immunology
- Opsonin Proteins / immunology
- Phagocytosis
- Saccharomyces cerevisiae / immunology
- Streptococcus / immunology
Citations
This article has been cited 2 times.- Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology.. Int J Mol Sci 2020 Feb 20;21(4).
- Wang J, Wang W, Pang Y. Saikosaponin A Inhibits LPS-Induced Endometritis in Mice Through Activating Nrf2 Signaling Pathway.. Inflammation 2018 Aug;41(4):1508-1514.
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