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Theriogenology2005; 64(3); 558-567; doi: 10.1016/j.theriogenology.2005.05.014

Cellular associations and the differential spermiogram: making sense of stallion spermatozoal morphology.

Abstract: Morphologic assessment of spermatozoa is one of the most objective measures in a Breeding Soundness Examination of a stallion. There are different systems for morphologic assessment of spermatozoa. The objectives of this article are to review spermatogenesis, describe clinical sample preparation, discuss previous methods of morphologic classification and explain the use of a differential spermiogram. The advantages of the differential spermiogram method of analysis are discussed, along with its use in delineating intrinsic and extrinsic disturbances in spermatogenesis. Case examples of specific cellular associations and their diagnostic relevance are discussed.
Publication Date: 2005-06-15 PubMed ID: 15953630DOI: 10.1016/j.theriogenology.2005.05.014Google Scholar: Lookup
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Summary

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This research investigates the process of spermatogenesis in stallions and the use of a differential spermiogram pertaining to stallion sperm morphology. The study examines the benefits of this methodology for identifying disruptions in spermatogenesis, with illustrations given from specific cases.

Spermatogenesis Overview

  • This research provides a comprehensive review of spermatogenesis, the process through which sperm cells are produced. This process is crucial in understanding stallion fertility and reproductive health, and provides insight into the underlying structures and mechanisms that guide sperm production.

Sample Preparation and Analysis

  • The article outlines the methodology for sample preparation to evaluate the morphology of spermatozoa. Techniques and protocols for sample preparation can significantly influence the results of any scientific investigation, hence their description is vital in understanding the results of the study.
  • The analysis of spermatozoa morphology, or the study of the structure and form of the sperm cells, is a critical aspect of the study. This is done using several systems or methods, each with its strengths and limitations.

Differential Spermiogram

  • The Differential Spermiogram is a method for analyzing sperm morphology. This innovative method, the focus of the article, is explained in detail – its working and the reasons for its application.
  • The differential spermiogram method allows for a more targeted and precise classification of spermatozoa morphology, leading to better understanding and diagnosis of potential spermatogenesis issues.
  • The differential spermiogram’s advantages over other sperm morphology methods are significantly highlighted, demonstrating the efficacy and precision of this method in identifying intrinsic and extrinsic disturbances in the spermatogenesis process, causing problems in sperm formation.

Case studies Examination

  • The research provides case examples of specific cellular associations, indicating the potential of the differential spermiogram method in diagnosing disturbances in sperm morphology. These cellular associations can be integral in forming a comprehensive understanding of spermatozoa structure, potentially revealing links to various fertility and reproductive issues in stallions.

Cite This Article

APA
Card C. (2005). Cellular associations and the differential spermiogram: making sense of stallion spermatozoal morphology. Theriogenology, 64(3), 558-567. https://doi.org/10.1016/j.theriogenology.2005.05.014

Publication

ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 64
Issue: 3
Pages: 558-567

Researcher Affiliations

Card, Claire
  • Department LACS, Western College of Veterinary Medicine, 52 Campus Drive, University of Saskatchewan, Saskatoon, Sask., Canada S7N 5B4. Claire.Card@usask.ca

MeSH Terms

  • Animals
  • Breeding
  • Female
  • Horse Diseases
  • Horses
  • Infertility, Male / veterinary
  • Male
  • Sexual Maturation
  • Spermatogenesis
  • Spermatozoa / abnormalities
  • Spermatozoa / ultrastructure

Citations

This article has been cited 3 times.
  1. Czubaszek M, Andraszek K, Banaszewska D, Walczak-Jędrzejowska R. The effect of the staining technique on morphological and morphometric parameters of boar sperm. PLoS One 2019;14(3):e0214243.
    doi: 10.1371/journal.pone.0214243pubmed: 30908553google scholar: lookup
  2. Phetudomsinsuk K, Sirinarumitr K, Laikul A, Pinyopummin A. Morphology and head morphometric characters of sperm in Thai native crossbred stallions. Acta Vet Scand 2008 Oct 22;50(1):41.
    doi: 10.1186/1751-0147-50-41pubmed: 18940014google scholar: lookup
  3. Morrell JM, Johannisson A, Dalin AM, Hammar L, Sandebert T, Rodriguez-Martinez H. Sperm morphology and chromatin integrity in Swedish warmblood stallions and their relationship to pregnancy rates. Acta Vet Scand 2008 Jan 7;50(1):2.
    doi: 10.1186/1751-0147-50-2pubmed: 18179691google scholar: lookup