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Home / Equine Research Bank / Vet Med Int / Changes in Adenylate Nucleotides Concentration and Na, K-ATP...
Veterinary medicine international2009; 2010; 987309; doi: 10.4061/2010/987309

Changes in Adenylate Nucleotides Concentration and Na, K-ATPase Activities in Erythrocytes of Horses in Function of Breed and Sex.

Abstract: The aim of this study was to examine the relationships between the concentrations of ATP, ADP, AMP (HPLC methods), total nucleotide pool (TAN), adenylate energy charge (AEC) and Na(+), K(+)-ATPase erythrocytic activities (by Choi's method) of horses as a function of breed and sex. The studies were conducted on 54 horses (stallions and mares) of different constitution types: breathing constitution (Wielkopolska and Hanoverian breed) and digestive constitution (Ardenian breed). Horse erythrocytes, independently of examined breed, present low ATP concentration in comparison to other mammal species while retaining relatively high AEC. Erythrocytes of breathing constitution type horses appear to have a more intensive glucose metabolism and a more efficient energetic metabolism when compared to digestive constitution type horses. The conclusions may be proven by significantly higher ATP concentration, higher TAN and significantly higher AEC in breathing constitution type horses compared to the digestive constitution type. Sex does not significantly influence adenine nucleotides concentration in the erythrocytes of the examined horses, however, stallions have slightly higher values in comparison to mares. A positive correlation was found between Na(+), K(+), -ATPase activity, ATP, ADP and AMP concentration and TAN in Wielkopolska and Ardenian breeds, which was not confirmed for the Hanoverian breed.
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Publication Date: 2009-11-11 PubMed ID: 20445796PubMed Central: PMC2859025DOI: 10.4061/2010/987309Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the differences in ATP, ADP, AMP concentrations and Na(+), K(+)-ATPase activities in the red blood cells (erythrocytes) of different horse breeds, based on their constitution type (breathing or digestive), and sex. It concludes that breathing constitution type horses have a more efficient energy metabolism than digestive constitution types, and that sex does not significantly affect these measurements in the studied horses.

Research Methodology

  • The study was conducted using 54 horses of different breed and sex, divided into breathing constitution types (represented by Wielkopolska and Hanoverian breeds) and digestive constitution types (represented by the Ardenian breed).
  • For each horse, concentrations of ATP, ADP, and AMP in their erythrocytes were measured using high performance liquid chromatography (HPLC) methods.
  • The total adenylate nucleotide pool (TAN) – the sum of all these molecules – and the adenylate energy charge (AEC) – a measure of cell energy status – were calculated.
  • The activity of the enzyme Na(+), K(+)-ATPase within the horse’s erythrocytes was also evaluated using Choi’s method. This enzyme is responsible for managing the concentration of sodium and potassium ions within the cells.

Key Findings

  • Horses, regardless of breed, have lower ATP concentrations in their erythrocytes compared to other mammalian species, but maintain relatively high AEC, indicating efficient energy metabolism.
  • Breathing constitution type horses have a more intensive glucose metabolism and a more efficient energetic metabolism when compared to digestive constitution type horses. This is demonstrated by their significantly higher ATP concentration, higher TAN, and significantly higher AEC.
  • Sex does not significantly influence the concentration of adenine nucleotides in the erythrocytes of horses, although stallions had slightly higher measurements than mares.
  • The study found a positive correlation between Na(+), K(+), -ATPase activity, ATP, ADP and AMP concentration, and TAN in Wielkopolska and Ardenian breeds. This correlation was not confirmed for the Hanoverian breed.

Conclusion

  • The findings of this study provide valuable insight into understanding the metabolic differences between horses of different constitution types and breeds. It could have potential implications for nutritional planning and health management of horses, geared to their specific breed and constitutional type characteristics.
  • The fact that sex does not significantly influence these metabolic parameters is also interesting, suggesting that, in some aspects, energy metabolism in horses may not be significantly influenced by gender.

Cite This Article

APA
Suska M, Skotnicka E. (2009). Changes in Adenylate Nucleotides Concentration and Na, K-ATPase Activities in Erythrocytes of Horses in Function of Breed and Sex. Vet Med Int, 2010, 987309. https://doi.org/10.4061/2010/987309

Publication

Veterinary medicine international
ISSN: 2042-0048
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2010
Pages: 987309

Researcher Affiliations

Suska, Maria
  • Department of Physiology, Faculty of Life Sciences, University of Szczecin, Piastów 40 B, av, bl. 6, 71-065 Szczecin, Poland.
Skotnicka, Ewa

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