Changes in coagulation and fibrinolysis in horses during exercise.
Abstract: Changes in clotting time (CT) and fibrinolytic activity (FA) were evaluated in 6 mature, female horses during exercise. Two trials were performed on consecutive days, using a randomized crossover design. Each mare was assigned to either an exercise trial or a control trial on the first day, and to the alternate trial 24 hours later. Mares exercised for 20 minutes on a treadmill at an elevation of 2 degrees and a velocity of 5 m/s. Venous blood samples were collected immediately before exercise, at 4, 8, 12, 16 and 20 minutes during exercise, and 15 minutes after cessation of exercise. Blood was placed into plain glass tubes for determination of CT, and into chilled, citrated tubes for determination of FA, plasminogen/plasmin complex activity (PLG), one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), and antithrombin-III (AT-III) activity. There were significant differences (P less than 0.05) between the control and exercise groups for CT, FA, and PLG. During exercise, clotting time decreased from 21.5 +/- 1.6 minutes to 9.9 +/- 1.6 minutes (mean +/- SD; P less than 0.05), without significant changes in OSPT, APTT, or AT-III. Fibrinolytic activity and PLG increased (P less than 0.05) during exercise. Changes in CT, FA, and PLG were significant at 4 minutes of exercise, remained altered until the end of exercise, and returned to baseline values by 15 minutes of recovery. Clotting time, OSPT, APTT, FA, AT-III, and PLG did not change (P greater than 0.05) during control trials.
Publication Date: 1990-09-01 PubMed ID: 2396779
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research looks at changes in blood clotting time and fibrinolytic activity in horses during exercise, indicating that clotting time decreases and fibrinolytic activity increases during a 20-minute exercise period.
Research Parameters and Design
- The study was conducted with 6 mature, female horses.
- The study was carried out in two trials on consecutive days, using a randomized crossover design to limit bias and control confounding effects.
- In the exercise trial, each mare was made to exercise for 20 minutes on a treadmill at an elevation of 2 degrees and a velocity of 5 m/s. In the control trial, the mares did not exercise.
- Blood samples were collected at specific times – before the exercise, at 4-minute intervals during exercise, and 15 minutes after the exercise.
- The blood samples were used to determine clotting time (CT), fibrinolytic activity (FA), plasminogen/plasmin complex activity (PLG), one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), and antithrombin-III (AT-III) activity.
Principal Findings
- The research found significant differences (P < 0.05) between the control and exercise groups for CT, FA, and PLG.
- During exercise, the clotting time significantly decreased from 21.5 +/- 1.6 minutes to 9.9 +/- 1.6 minutes, with no significant changes noticed in OSPT, APTT, or AT-III compartments.
- There was a significant increase in fibrinolytic activity and PLG during exercise.
- The changes in CT, FA, and PLG became observable at 4 minutes of exercise, remained altered until the end of exercise, and returned to baseline values by 15 minutes of recovery.
- There were no significant changes in the CT, OSPT, APTT, FA, AT-III, and PLG during the control trials.
Research Implications
- The research demonstrates clear physiological changes in horses’ coagulation and fibrinolytic parameters during exercise, providing valuable insights for veterinary practitioners and those working in equine sports medicine.
- The findings could inform the development of protocols for exercise and recovery for horses aiming to optimize their coagulation system’s responses.
Cite This Article
APA
McKeever KH, Hinchcliff KW, Kociba GJ, Reed SM, Muir WW.
(1990).
Changes in coagulation and fibrinolysis in horses during exercise.
Am J Vet Res, 51(9), 1335-1339.
Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Ohio State University, Columbus 43210.
MeSH Terms
- Animals
- Blood Coagulation / physiology
- Female
- Fibrinolysis / physiology
- Heart Rate
- Horses / blood
- Horses / physiology
- Physical Exertion
- Random Allocation
- Time Factors
- Whole Blood Coagulation Time / veterinary
Citations
This article has been cited 4 times.- Miglio A, Falcinelli E, Mezzasoma AM, Cappelli K, Mecocci S, Gresele P, Antognoni MT. Effect of First Long-Term Training on Whole Blood Count and Blood Clotting Parameters in Thoroughbreds. Animals (Basel) 2021 Feb 9;11(2).
- Piccione G, Bazzano M, Giannetto C, Marafioti S, Fazio F. Training-induced changes in clotting parameters of athletic horses. J Vet Sci 2014;15(1):45-9.
- Perez-Moreno CI, Couëtil LL, Pratt SM, Ochoa-Acuña HG, Raskin RE, Russell MA. Effect of furosemide and furosemide-carbazochrome combination on exercise-induced pulmonary hemorrhage in Standardbred racehorses. Can Vet J 2009 Aug;50(8):821-7.
- Miglio A, Falcinelli E, Cappelli K, Mecocci S, Mezzasoma AM, Antognoni MT, Gresele P. Effect of Regular Training on Platelet Function in Untrained Thoroughbreds. Animals (Basel) 2024 Jan 27;14(3).
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