Changes in gas composition and acid-base values of venous blood samples stored under different conditions in 4 domestic species.
Abstract: The effect of storage temperature and time on blood gas and acid-base values has been investigated intensively in cattle and dogs; however, data are lacking in other species. Objective: The aim of our study was to evaluate changes in gas composition and acid-base values in venous blood stored at different temperatures and for different times in 4 domestic species in Italy. Methods: Blood samples from Comisana sheep (n = 10), Maltese goats (n = 10), Ragusana donkeys (n = 10), and Thoroughbred horses (n = 10) were analyzed after storage at 23 degrees C (room temperature) for 15 minutes (group I), 23 degrees C for 1 hour (group II), 37 degrees C for 8 hours (group III), and 4 degrees C for 24 hours (group IV). Results were analyzed using a 1-way repeated measures ANOVA. Results: In all species no statistically significant differences in pH values were present in samples stored at 4 degrees C for 24 hours. This also was true for PCO2 in all species except the horse. Except for HCO3- concentration in the horse, significant changes in PO2, HCO3- concentration, base excess, and the standard bicarbonate concentration were observed for all species in samples stored at 4 degrees C. In samples stored for only 1 hour at room temperature, significant changes in most analytes were detected. Conclusions: The results of this study underline the need for rapid assessment of acid-base samples, because any delay, even for 1 hour, may affect the results.
Publication Date: 2007-11-29 PubMed ID: 18041702DOI: 10.1111/j.1939-165x.2007.tb00441.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article examines how the storage conditions affect the gas composition and acid-base values in venous blood of four domestic species in Italy: Comisana sheep, Maltese goats, Ragusana donkeys, and Thoroughbred horses.
Objective and Methods of the Research
- The study is aimed at investigating how different storage temperatures and time periods impact the gas composition and acid-base values in venous blood of four domestic species. This comes as an effort to fill the data void present in species apart from cattle and dogs.
- Blood samples were collected from 10 individuals of each species and stored under varying conditions:
- 23 degrees C (room temperature) for 15 minutes
- 23 degrees C for 1 hour
- 37 degrees C for 8 hours
- 4 degrees C for 24 hours
- A 1-way repeated measures ANOVA was used for the statistical analysis of the results.
Results of the Research
- The pH values showed no significant difference in any species, for samples stored at 4 degrees C for 24 hours. This observation also held true for PCO2 in all species, except for horses.
- Spikes in PO2, HCO3- concentration, base excess and standard bicarbonate concentration were noted in all samples stored at 4 degrees C. Horses, however, did not display a significant change in HCO3- concentration.
- Significant changes in most analytes were recorded in samples left at room temperature for only 1 hour.
Conclusion
- The findings of the study highlight the importance of timely assessment of acid-base samples. This is because delays, even as short as 1 hour, can significantly alter the results.
Cite This Article
APA
Piccione G, Bertolucci C, Grasso F, Guidice E.
(2007).
Changes in gas composition and acid-base values of venous blood samples stored under different conditions in 4 domestic species.
Vet Clin Pathol, 36(4), 358-360.
https://doi.org/10.1111/j.1939-165x.2007.tb00441.x Publication
Researcher Affiliations
- Department of Morphology, Biochemistry, Physiology and Animal Production, Faculty of Veterinary Medicine, University of Ferrara, Italy. guiseppe.piccione@unime.it
MeSH Terms
- Animals
- Blood Gas Analysis / veterinary
- Equidae / blood
- Goats / blood
- Hydrogen-Ion Concentration
- Sheep / blood
- Specimen Handling / veterinary
- Temperature
- Time Factors
Citations
This article has been cited 4 times.- Hamilton MT, Finger JW Jr, Winzeler ME, Tuberville TD. Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser. Conserv Physiol 2016;4(1):cov065.
- Hussein HA, Aamer AA. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Vet J 2013;3(1):1-7.
- Alberghina D, Casella S, Giannetto C, Marafioti S, Piccione G. Effect of storage time and temperature on the total protein concentration and electrophoretic fractions in equine serum. Can J Vet Res 2013 Oct;77(4):293-6.
- Ellis J, Waldner C, Gow S, Jackson M. Relationship of the extent of pulmonary lesions to the partial pressure of oxygen and the lactate concentration in arterial blood in calves experimentally infected with bovine respiratory syncytial virus. Can J Vet Res 2013 Jul;77(3):205-10.
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