Changes in plasma progesterone levels during storage of heparinized whole blood from cow, horse, dog and pig.
Abstract: Progesterone concentrations in heparinized plasma harvested immediately after blood collection were compared with levels obtained after storage of the corresponding whole blood for 2 h, 4 h, 6 h, 1 day, 2 days and 5 days at room temperature and in a refrigerator. The blood was taken during the luteal phase from 4 dogs, 4 horses, 4 pigs and 8 cows. For 4 cows the storage time was extended to 9 and 20 days. No significant effect of whole blood storage time on plasma progesterone concentrations could be shown for dogs or pigs. For the horse a slight but significant decrease was demonstrated when the blood was kept at room temperature. For the cow, however, a dramatic decrease was observed even when the blood was stored in the refrigerator. Following incubation of cow’s blood at room temperature, progesterone levels were close to zero after 1–2 days. By further extending the storage period, a reappearance of assayable progesterone could be elicited. For all species it was found that the storage of at room temperature for 5–9 days did not change the progesterone concentrations. Progesteronkoncentrationen i heparinplasma avskiljd omedelbart efter provtagningen jämfördes med nivåer erhållna efter lagring av motsvarande helblod i 2 h, 4 h, 6 h, 1 dag, 2 dagar och 5 dagar i rumstemperatur och i kylskåp. Blod togs från 4 hundar, 4 hästar, 4 grisar och 8 kor som alla befann sig i luteal fas. För 4 av korna utsträcktes lagringstiden till 9 och 20 dagar. Hos hund och gris påverkades inte plasmaprogesteronkoncentrationen märkbart vid lagring av helblodet. Hos häst påvisades en relativt lindrig men statistiskt signifikant ned-gång efter lagring i rustemperatur. För ko künde däremot konstateras en dramatisk nedgång i plasmaprogesteronkoncentrationen även efter kylskåpslagring av blodet. Vid rumslagring var progesteronnivåerna nära noll efter 1–2 dagar för att därefter åter öka. För alla undersökta djurslag gällde att lagring av i rumstempertur i 5–9 dagar inte påverkade progesteronkoncentrationen.
Publication Date: 1982-01-01 PubMed ID: 7113859PubMed Central: PMC8295762DOI: 10.1186/BF03546815Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research investigates the effect of storage time and temperature on plasma progesterone levels in the heparinized whole blood obtained from cows, horses, dogs, and pigs, revealing striking decreases observed particularly in cow blood with extended storage times.
Methodology
- The research involved collecting heparinized whole blood from 4 dogs, 4 horses, 4 pigs and 8 cows during the luteal phase.
- Progesterone concentrations in plasma harvested right after collection were compared against levels determined after storing the corresponding whole blood for specific periods (2 h, 4 h, 6 h, 1 day, 2 days and 5 days) at room temperature and inside a refrigerator.
- For 4 cows, the storage period was extended to 9 and 20 days.
Key Findings
- No significant changes in plasma progesterone concentrations were observed for dogs or pigs regardless of whole blood storage time.
- In horses, there was a minor but statistically significant progesterone decrease noted when the blood was kept at room temperature.
- For cows, an intense decrease was observed even when the blood was stored inside a refrigerator.
- Progesterone levels in cow’s blood decreased nearly to zero after 1-2 days of storage at room temperature. However, further prolongation of the storage period resulted in the reappearance of measurable progesterone.
- For all the animal species, keeping their blood at room temperature for 5-9 days did not significantly change the progesterone concentrations. This suggests the effect of storage temperature and duration on progesterone concentration may vary among different animal species.
Implications
- The findings highlight the importance of considering blood storage conditions and durations when studying progesterone concentration in the blood of different animals, especially in cows, to avoid erroneous interpretations.
- The variability observed in the stability of plasma progesterone levels among different animal species should be accounted for in future studies to ensure the accurate assessment of hormonal states.
Cite This Article
APA
Oltner R, Edqvist LE.
(1982).
Changes in plasma progesterone levels during storage of heparinized whole blood from cow, horse, dog and pig.
Acta Vet Scand, 23(1), 1-8.
https://doi.org/10.1186/BF03546815 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Blood Specimen Collection / veterinary
- Cattle / blood
- Dogs / blood
- Female
- Horses / blood
- Progesterone / blood
- Radioimmunoassay
- Swine / blood
- Temperature
- Time Factors
References
This article includes 9 references
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- Helwig JT, Council KA. SAS User’s Guide. SAS Institute, Inc., Raleigh, N.G. 1979.
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- Owens RE, Atkins DT, Rahe CH, Fleeger JL, Harms PG. Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood.. Theriogenology 1980 Apr;13(4):305-9.
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Citations
This article has been cited 4 times.- de Castro T, Valdez L, Rodriguez M, Benquet N, Rubianes E. Decline in assayable progesterone in bovine serum under different storage conditions. Trop Anim Health Prod 2004 May;36(4):381-4.
- Masunda B, Mutisi C, Hamudikuwanda H, Agumbah JG. The concentration of faecal progestins during the oestrous cycle in Nkone cows and the effect of duration of storage of faecal samples at room temperature on faecal progestin levels. Trop Anim Health Prod 1999 Dec;31(6):373-81.
- Mukasa-Mugerwa E, Bekele T, Kasali OB. The effect of handling methods on subsequent plasma progesterone levels in sheep. Vet Res Commun 1989;13(1):75-9.
- Abal MA, Ghezzi M, Quiroga M, Solana H, Auza N. Concentrations of progesterone during storage of whole blood from llama (Lama glama): effects of anticoagulants, storage time and temperature. Acta Vet Scand 1996;37(1):123-5.
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