Changes in Serum Antibody Levels after Vaccination for Strangles and after Intranasal Challenge with Streptococcus equi subsp. equi in Horses.
Abstract: In this study, to evaluate the influence of strangles vaccination on serological test results, we investigated the changes in strangles serum antibody levels in horses after vaccination and subsequent intranasal challenge with S. equi. The horses were vaccinated for strangles with either a component vaccine (Group C) or a live vaccine (Group L). We measured changes in strangles serum antibody levels weekly for 20 weeks after vaccinating horses twice for strangles over a 3-week interval, and for 7 weeks after intranasal challenge with S. equi in the same horses. Serum antibody responses to the proline-glutamic acid-proline-lysine (PEPK) antigen with five repetitions (PEPK-5R) were higher at all times (up to 2.4-fold) following vaccination in Group C than in Group L, and the value peaked at 2.9-fold above the initial value after the second vaccination in Group C horses. However, the value was lower than that in horses infected with S. equi, and it gradually decreased, reaching the initial (week 0) value by the 15th week. Serum antibody responses to PEPK-5R after challenge with S. equi increased in both groups of horses, but the value tended to be lower than that reported for unvaccinated horses. In addition, the average value in Group C was 2.6-fold higher than that of Group L. These results suggest the serum antibody responses of horses infected with S. equi varies according to the type of vaccine with which they have been vaccinated. Although the serological diagnostic test for strangles in which PEPK-5R is used as an antigen is effective for the investigation of serum antibodies to strangles in vaccinated horses, the present data suggest it is necessary to consider the vaccination history when interpreting the results.
Publication Date: 2010-10-22 PubMed ID: 24833976PubMed Central: PMC4013967DOI: 10.1294/jes.21.33Google Scholar: Lookup
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Summary
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The study investigates the changes in strangles serum antibodies in horses vaccinated for strangles and challenged intranasally with S. equi bacteria. The different types of vaccines used indicated variations in antibody response, highlighting the need to consider the vaccination history when interpreting serological diagnostic test results for strangles.
Overview of the Study
- The research was conducted to examine the effect of a specific strangles vaccination on serological test results in horses.
- The study aimed to understand the changes in strangles serum antibody levels in horses after vaccination and subsequent intranasal challenge with Streptococcus equi (S. equi), the bacteria causing strangles.
- The horses were vaccinated with either a component vaccine (Group C) or a live vaccine (Group L).
Methods
- The antibodies in horses’ serum were tested weekly for 20 weeks after administering two rounds of strangles vaccines over a three-week interval.
- The horses were challenged intranasally with S. equi to study the resultant changes in serum antibody levels for seven weeks.
Results
- The study found that the antibody responses to the proline-glutamic acid-proline-lysine (PEPK) antigen were consistently higher in Group C horses after vaccination.
- However, these levels were still lower than those seen in horses naturally infected with S. equi.
- Antibody levels tended to decline and revert to the initial value by the 15th week.
Post-Iontranasal Challenge Results
- After the horses were challenged with S. equi, both groups showed an increased antibody response, but these values were still lower than those reported for unvaccinated horses.
- The average value in Group C was discovered to be 2.6-fold higher than that of Group L.
Interpretation and Conclusion
- The results suggest that the type of strangles vaccine used could impact the serum antibody responses upon S. equi infection.
- The study concluded that though the diagnostic test involving PEPK-5R as an antigen is efficient in investigating serum antibodies to strangles in vaccinated horses, it is crucial to factor in the vaccination history when interpreting the results.
Cite This Article
APA
Hobo S, Niwa H, Anzai T, Jones JH.
(2010).
Changes in Serum Antibody Levels after Vaccination for Strangles and after Intranasal Challenge with Streptococcus equi subsp. equi in Horses.
J Equine Sci, 21(3), 33-37.
https://doi.org/10.1294/jes.21.33 Publication
Researcher Affiliations
- Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Department of Surgical and Radiological Science, Giannini Equine Athletic Performance Laboratory, School of Veterinary Medicine, University of California, Davis, CA 95616, U.S.A.
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