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Changes in steady-state concentrations of messenger ribonucleic acids in luteal tissue during prostaglandin F2alpha induced luteolysis in mares.
Abstract: Transvaginal ultrasound-guided luteal biopsy was used to evaluate the effects of prostaglandin (PG)F2alpha on steady-state concentrations of mRNA for specific genes that may be involved in regression of the corpus luteum (CL). Eight days after ovulation (Hour 0), mares (n=8/group) were randomized into three groups: control (no treatment or biopsy), saline+biopsy (saline treatment at Hour 0 and luteal biopsy at Hour 12), or PGF2alpha+biopsy (5mg PGF2alpha at Hour 0 and luteal biopsy at Hour 12). The effects of biopsy on CL were compared between the controls (no biopsy) and saline+biopsy group. At Hour 24 (12h after biopsy) there was a decrease in circulating progesterone in saline group to 56% of pre-biopsy values, indicating an effect of biopsy on luteal function. Mean plasma progesterone concentrations were lower (P<0.001) at Hour 12 in the PG group compared to the other two groups. The relative concentrations of mRNA for different genes in luteal tissue at Hour 12 was quantified by real time PCR. Compared to saline-treated mares, treatment with PGF2alpha increased mRNA for cyclooxygenase-2 (Cox-2, 310%, P<0.006), but decreased mRNA for LH receptor to 44% (P<0.05), steroidogenic acute regulatory protein to 22% (P<0.001), and aromatase to 43% (P<0.1) of controls. There was no difference in mRNA levels for PGF2alpha receptor between PG and saline-treated groups. Results indicated that luteal biopsy alters subsequent luteal function. However, the biopsy approach was effective for collecting CL tissue for demonstrating dynamic changes in steady-state levels of mRNAs during PGF2alpha-induced luteolysis. Increased Cox-2 mRNA concentrations suggested that exogenous PGF2alpha induced the synthesis of intraluteal PGF2alpha. Thus, the findings are consistent with the concept that an intraluteal autocrine loop augments the luteolytic effect of uterine PGF2alpha in mares.
Publication Date: 2005-11-22 PubMed ID: 16298275DOI: 10.1016/j.anireprosci.2005.02.008Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research examined how prostaglandin F2alpha affects the concentrations of specific genes involved in the regression of the corpus luteum in mares.
Methodology
In this study, the researchers used a transvaginal ultrasound-guided luteal biopsy to assess the effects of prostaglandin (PG) F2alpha on the steady-state concentrations of mRNA for specific genes believed to be involved in the regression of the corpus luteum (a temporary gland formed in the ovary after ovulation).
- Eight days after ovulation, the mares were divided into three groups:
- The control group received no treatment or biopsy
- The saline+biopsy group received saline treatment and then underwent a luteal biopsy 12 hours later
- The PGF2alpha+biopsy group received 5mg of PGF2alpha treatment and then had a luteal biopsy 12 hours later.
Results
The effects of the biopsy were compared between the control group and those in the saline+biopsy group, and the results showed that there was a decrease in circulating progesterone (a key hormone produced by the corpus luteum) in the saline group to 56% of pre-biopsy values. This suggested that the biopsy had an impact on the functioning of the luteum.
- It was found that the mean plasma progesterone concentrations were significantly lower at Hour 12 in the PGF2alpha group compared to the other two groups.
- The concentration of mRNA for different genes within the luteal tissue was quantified using real-time PCR (a method to amplify and simultaneously quantify a targeted DNA molecule).
- When compared to the saline-treated mares, treatment with PGF2alpha increased mRNA for cyclooxygenase-2 (Cox-2), suggesting that exogenous (externally administered) PGF2alpha induced the synthesis of intraluteal PGF2alpha.
- However, mRNA for LH receptors, steroidogenic acute regulatory protein, and aromatase were all decreased as a result of the treatment.
Conclusion
In summary, the study indicates that luteal biopsy alters subsequent luteal function; nevertheless, the biopsy approach proved effective for collecting corpus luteum tissue for demonstrating dynamic changes in steady-state mRNA levels during PGF2alpha-induced luteolysis (the degradation of the corpus luteum). The increase in Cox-2 mRNA concentrations also suggest that exogenous PGF2alpha generates additional intraluteal PGF2alpha – providing consistent evidence for the concept that an intraluteal autocrine loop (a signalling system where a cell targets itself) amplifies the luteolytic effect of uterine PGF2alpha in mares.
Cite This Article
APA
Beg MA, Gastal EL, Gastal MO, Ji S, Wiltbank MC, Ginther OJ.
(2005).
Changes in steady-state concentrations of messenger ribonucleic acids in luteal tissue during prostaglandin F2alpha induced luteolysis in mares.
Anim Reprod Sci, 90(3-4), 273-285.
https://doi.org/10.1016/j.anireprosci.2005.02.008 Publication
Researcher Affiliations
- Department of Animal Health and Biomedical Sciences, 1656 Linden Drive, University of Wisconsin-Madison, WI 53706, USA.
MeSH Terms
- Animals
- Aromatase / genetics
- Biopsy / veterinary
- Corpus Luteum / chemistry
- Cyclooxygenase 2 / genetics
- Dinoprost / pharmacology
- Female
- Horses
- Luteolysis / drug effects
- Luteolysis / genetics
- Ovulation
- Phosphoproteins / genetics
- Progesterone / blood
- RNA, Messenger / analysis
- Receptors, LH / genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
Citations
This article has been cited 2 times.- Ishak GM, Bashir ST, Dutra GA, Gastal GDA, Gastal MO, Cavinder CA, Feugang JM, Gastal EL. In vivo antral follicle wall biopsy: a new research technique to study ovarian function at the cellular and molecular levels. Reprod Biol Endocrinol 2018 Jul 28;16(1):71.
- Atli MO, Bender RW, Mehta V, Bastos MR, Luo W, Vezina CM, Wiltbank MC. Patterns of gene expression in the bovine corpus luteum following repeated intrauterine infusions of low doses of prostaglandin F2alpha. Biol Reprod 2012 Apr;86(4):130.
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