Changes of mRNA, miRNA and lncRNA expression contributing to skeletal muscle differences between fetus and adult Mongolian horses.
Abstract: The growth and development of myofibers, as the fundamental units comprising muscle tissue, and their composition type are indeed among the most crucial factors influencing skeletal muscle types. Muscle fiber adaptation is closely associated with alterations in physiological conditions. Muscle fiber types undergo dynamic changes in fetus and adult horses. Our aim is to investigate the mechanisms influencing the differences in muscle fiber types between fetal and adult stages of Mongolian horses. The study investigated the distribution of muscle fiber types within longissimus dorsi muscle of fetus and adult Mongolian horses. A total of 652 differentially expressed genes (DEGs), 476 Differentially expressed lncRNAs (DELs), and 174 Differentially expressed miRNAs (DEMIRs) were identified using deep RNA-seq analysis. The results of functional analysis reveal the transformations in muscle fiber type from the fetal to adult stage in Mongolian horses. The up-regulated DEGs were implicated in the development and differentiation of muscle fibers, while down-regulated DEGs were associated with muscle fiber contraction, transformation, and metabolism. Additionally, connections between non-coding RNA and mRNA landscapes were identified based on their functional alterations, some non-coding RNA target genes may be associated with immunity. These data have broadened our understanding of the specific roles and interrelationships among regulatory molecules involved in Mongolian horse development, this provides new perspectives for selecting and breeding superior individuals and for disease prevention.
Copyright © 2024 Elsevier Inc. All rights reserved.
Publication Date: 2024-07-16 PubMed ID: 39180870DOI: 10.1016/j.cbd.2024.101294Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the differences in muscle fiber types between fetal and adult stages of Mongolian horses. This is done by examining the distribution of muscle fiber types and identifying changes in the expression of certain genes, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) using deep RNA-seq analysis.
Research Design and Methodology
- The researchers focused on the longissimus dorsi muscle of fetus and adult Mongolian horses. The longissimus dorsi muscle is a large muscle that runs along the horse’s back, and is crucial to the horse’s movement and strength.
- They applied a technique called deep RNA-seq analysis. This is a next-generation sequencing technology that provides insights into the transcriptome, the complete set of all the RNA molecules in one cell or a population of cells. In this case, it was used to compare gene expressions in the fetal and adult muscle cells.
Results and Findings
- The researchers identified 652 differentially expressed genes (DEGs), 476 differentially expressed lncRNAs (DELs), and 174 differentially expressed miRNAs (DEMiRs) between the two stages of development.
- Up-regulated DEGs were involved in the development and differentiation of muscle fibers during the transition from fetus to adult stage, whereas down-regulated DEGs were tied to muscle fiber contraction, transformation, and metabolism.
- Non-coding RNA and mRNA landscapes were also connected based on their functional changes, suggesting that some non-coding RNA target genes could be involved in immunity.
Implications and Potential Applications
- The study contributes towards an improved understanding of the developmental processes that underpin the growth and differentiation of muscle fibers in Mongolian horses.
- The findings could potentially inform the selection of superior individuals for breeding, and can be used for disease prevention strategies. Understanding the changes in muscle fiber types may also offer valuable insights into health conditions such as muscle-related diseases, metabolic disorders or developmental issues in horses.
Cite This Article
APA
Ding W, Gong W, Liu H, Hu H, Shi L, Ren X, Cao Y, Zhang A, Shi X, Li Z, Bou T, Dugarjaviin M, Bai D.
(2024).
Changes of mRNA, miRNA and lncRNA expression contributing to skeletal muscle differences between fetus and adult Mongolian horses.
Comp Biochem Physiol Part D Genomics Proteomics, 52, 101294.
https://doi.org/10.1016/j.cbd.2024.101294 Publication
Researcher Affiliations
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
- Key Laboratory of Equus Germplasm Innovation (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China. Electronic address: baidongyi1983@163.com.
MeSH Terms
- Animals
- Horses / genetics
- MicroRNAs / genetics
- RNA, Long Noncoding / genetics
- RNA, Messenger / genetics
- Muscle, Skeletal / metabolism
- Fetus / metabolism
- Gene Expression Regulation, Developmental
- Gene Expression Profiling
Conflict of Interest Statement
Declaration of competing interest The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
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