Characterisation and intracellular labelling of mesenchymal stromal cells derived from synovial fluid of horses and sheep.
Abstract: Multipotent mesenchymal stromal cells (MSCs) derived from synovial fluid (SF) are considered to be a promising cell type for therapeutic applications in joint disease. However, despite their potential relevance for clinical and experimental studies, there is insufficient knowledge about SF-derived MSCs isolated from horses and sheep. In this study, cells were recovered from healthy SF and bone marrow (BM) of sheep, and from healthy and osteoarthritic SF of horses. Ovine SF-MSCs were used to assess the efficiency of intracellular labelling with quantum dots (QDs). Colony forming units, generation times, trilineage differentiation potential and expression of CD73, CD90 and CD105 at mRNA level were assessed. QD labelling was efficient, with >98% positive cells directly after labelling at 10 nmol/L and >95% positive cells directly after labelling at 2 nmol/L. The label decreased over 7 days of culture, with more persistence at the higher labelling concentration. No significant differences in proliferation were observed. All MSCs had trilineage differentiation potential, but adipogenesis was more distinct in equine samples and chondrogenesis was most pronounced in ovine SF-MSCs. CD73, CD90 and CD105 were expressed in equine and ovine MSCs.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Publication Date: 2017-02-24 PubMed ID: 28410670DOI: 10.1016/j.tvjl.2017.02.006Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the characteristics and potential uses of mesenchymal stromal cells (MSCs) derived from synovial fluid in horses and sheep, with an aim to apply this knowledge in addressing joint diseases.
Study Objective
The research was conducted to overcome the limited information about SF-derived MSCs from horses and sheep. The research endeavored to label and characterize these cells in the hope that future cell-based therapies for joint diseases can be developed.
Methods
- Mesenchymal stromal cells were obtained from the synovial fluid and bone marrow of healthy sheep, as well as from the synovial fluid of healthy and osteoarthritic horses.
- These MSCs were then labelled using quantum dots (QDs) to observe their intracellular movements and behaviour.
- The researchers also conducted several assessments to evaluate various cellular attributes like colony forming units, generation times, along with trilineage differentiation potential.
- The expression of genes such as CD73, CD90, and CD105 at mRNA level were also assessed.
Results
- The efficiency of quantum dot labelling was high, with over 95 to 98% success rate at 2 to 10 nmol/L concentrations.
- Though the label started to decrease over a period of a week, it was more persistent when applied at higher concentrations.
- The study found no significant differences in cell proliferation before and after labelling.
- All the MSCs had the ability to differentiate into three different types of cells, with adipogenesis (fat cell formation) being more distinct in horse samples, and chondrogenesis (cartilage cell formation) being most pronounced in sheep-derived MSCs.
- The gene expressions for CD73, CD90 and CD105 were present in MSCs of both horses and sheep.
Implications
The findings of this research provide a detailed characterisation of mesenchymal stromal cells derived from the synovial fluid of horses and sheep. The information enables the researchers to better understand the nature and behavior of these cells, which may propel further investigations into the development and optimization of cell-based therapies for joint diseases in animals and potentially, humans.
Cite This Article
APA
Burk J, Glauche SM, Brehm W, Crovace A, Francioso E, Hillmann A, Schubert S, Lacitignola L.
(2017).
Characterisation and intracellular labelling of mesenchymal stromal cells derived from synovial fluid of horses and sheep.
Vet J, 222, 1-8.
https://doi.org/10.1016/j.tvjl.2017.02.006 Publication
Researcher Affiliations
- Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Philipp-Rosenthal-Str. 55, Leipzig 04103, Germany; Institute of Veterinary Physiology, University of Leipzig, An den Tierkliniken 7, Leipzig 04103, Germany. Electronic address: burk@rz.uni-leipzig.de.
- Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Philipp-Rosenthal-Str. 55, Leipzig 04103, Germany.
- Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Philipp-Rosenthal-Str. 55, Leipzig 04103, Germany; Large Animal Clinic for Surgery, University of Leipzig, An den Tierkliniken 21, Leipzig 04103, Germany.
- Department of Emergency and Organ Transplants (DEOT), University of Bari 'Aldo Moro', Strada Provinciale per Casamassima km. 3, Valenzano 70010, Italy.
- Department of Emergency and Organ Transplants (DEOT), University of Bari 'Aldo Moro', Strada Provinciale per Casamassima km. 3, Valenzano 70010, Italy.
- Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Philipp-Rosenthal-Str. 55, Leipzig 04103, Germany.
- Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Philipp-Rosenthal-Str. 55, Leipzig 04103, Germany.
- Department of Emergency and Organ Transplants (DEOT), University of Bari 'Aldo Moro', Strada Provinciale per Casamassima km. 3, Valenzano 70010, Italy.
MeSH Terms
- Animals
- Cell Differentiation
- Cell Separation / veterinary
- Horses / anatomy & histology
- Mesenchymal Stem Cells / cytology
- Multipotent Stem Cells / cytology
- Quantum Dots
- Sheep / anatomy & histology
- Synovial Fluid / cytology
Citations
This article has been cited 3 times.- Roth SP, Burk J, Brehm W, Troillet A. MSC in Tendon and Joint Disease: The Context-Sensitive Link Between Targets and Therapeutic Mechanisms.. Front Bioeng Biotechnol 2022;10:855095.
- Khan MR, Smith RK, David F, Lam R, Hughes G, De Godoy R, Carr AJ, Goodship AE, Dudhia J. Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy.. J Orthop Res 2020 Jan;38(1):128-138.
- Neybecker P, Henrionnet C, Pape E, Mainard D, Galois L, Loeuille D, Gillet P, Pinzano A. In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells.. Stem Cell Res Ther 2018 Nov 28;9(1):329.
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