Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals.
Abstract: Strains of Vagococcus fluvialis, a species of Gram-positive catalase-negative cocci, related to the genera Enterococcus and Carnobacterium, were isolated from various lesions of pigs, from lesions and tonsils of cattle and cats and from tonsils of a horse. Most lesion strains were isolated in mixed culture from animals with disease conditions unrelated to coccal infection. Certain differences with the species description of Vagococcus fluvialis were found: only a proportion of the strains was motile; many strains gave positive reactions to Voges-Proskauer, alkaline phosphatase and leucine arylamidase tests or produced acid from galactose and D-tagatose. SDS-PAGE of whole-cell protein patterns, however, confirmed the phenotypic identification. Guidelines for identification of Vagococcus fluvialis are given and an emended description of the species is proposed.
Publication Date: 1994-10-01 PubMed ID: 7989264DOI: 10.1111/j.1365-2672.1994.tb03436.xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study examines the characteristics of V. fluvialis strains, a type of bacteria, which were found in various domestic animals. The research also provides guidelines for recognizing this bacteria and proposes a revised description of the species.
Study Overview
In this study, the researchers isolated strains of Vagococcus fluvialis, an anaerobic bacteria from the family of cocci, from domestic animals like pigs, cattle, cats, and a horse. These strains were found in various lesions and tonsils, and most were found in mixed culture, meaning alongside other bacteria.
Findings
- The researchers noted certain dissimilarities with the existing description of Vagococcus fluvialis. This includes the fact that not all the strains isolated were motile, or capable of moving independently.
- Additionally, several strains gave positive reactions to various lab tests such as the Voges-Proskauer, alkaline phosphatase, and leucine arylamidase. These are biochemical tests used to distinguish different types of bacteria.
- Some strains were also seen to produce acid from certain sugars, another distinguishing characteristic used to identify bacteria.
- Despite these differences, the Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the whole-cell protein patterns confirmed the phenotypic identification. This is a method used to separate proteins by their electrophoretic mobility, which generally reflects the biological variations between species.
Conclusions
- The researchers provide guidelines for identifying Vagococcus fluvialis and propose an updated description of the species. This would help in correctly identifying the strains of this bacteria in different animals or environment.
- The study adds to our understanding of Vagococcus fluvialis, potentially enabling better diagnosis and treatment for illnesses caused by this bacterium.
Cite This Article
APA
Pot B, Devriese LA, Hommez J, Miry C, Vandemeulebroecke K, Kersters K, Haesebrouck F.
(1994).
Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals.
J Appl Bacteriol, 77(4), 362-369.
https://doi.org/10.1111/j.1365-2672.1994.tb03436.x Publication
Researcher Affiliations
- Laboratory of Microbiology, Faculty of Sciences, Universiteit Gent, Belgium.
MeSH Terms
- Animals
- Animals, Domestic / microbiology
- Bacterial Proteins / isolation & purification
- Carbohydrate Metabolism
- Catalase / metabolism
- Cats
- Cattle
- Cell Division
- Cell Movement
- Electrophoresis, Polyacrylamide Gel
- Enterococcus / classification
- Enterococcus / isolation & purification
- Enterococcus / metabolism
- Gram-Positive Cocci / classification
- Gram-Positive Cocci / isolation & purification
- Gram-Positive Cocci / metabolism
- Horses
- Phenotype
- Species Specificity
- Swine
Citations
This article has been cited 11 times.- Rosenbergová Z, Oftedal TF, Ovchinnikov KV, Thiyagarajah T, Rebroš M, Diep DB. Identification of a Novel Two-Peptide Lantibiotic from Vagococcus fluvialis. Microbiol Spectr 2022 Aug 31;10(4):e0095422.
- Giannattasio-Ferraz S, Ene A, Maskeri L, Oliveira AP, Barbosa-Stancioli EF, Putonti C. Vagococcus fluvialis isolation and sequencing from urine of healthy cattle. G3 (Bethesda) 2021 Jan 18;11(1).
- Al-Ahmad A, Pelz K, Schirrmeister JF, Hellwig E, Pukall R. Characterization of the first oral vagococcus isolate from a root-filled tooth with periradicular lesions. Curr Microbiol 2008 Sep;57(3):235-8.
- Teixeira LM, Carvalho MG, Merquior VL, Steigerwalt AG, Brenner DJ, Facklam RR. Phenotypic and genotypic characterization of Vagococcus fluvialis, including strains isolated from human sources. J Clin Microbiol 1997 Nov;35(11):2778-81.
- Vandamme P, Pot B, Gillis M, de Vos P, Kersters K, Swings J. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 1996 Jun;60(2):407-38.
- Averaimo D, Defourny SVP, Alessiani A, Rulli M, Chiaverini A, Di Domenico M, Mangone I, Pompilii C, Piersanti V, Giancristofaro R, Ricci L, Petrini A. Vagococcus lutrae Isolation in a Cat with Feline Urological Syndrome in Italy: A Case Report. Microorganisms 2025 Aug 29;13(9).
- Shahapur PR, Shahapur RP, Veggalam S, Kandi V. A Report of a Rare Case of Vagococcus fluvialis Isolated From Urine: Clinical Significance of Vagococcus Species With an Update of the Available Literature. Cureus 2024 Oct;16(10):e71620.
- Gonzalez-Fandos E, da Silva Guedes J. Microbiological Quality and Antibiotic Resistance of Relevant Bacteria from Horsemeat. Microorganisms 2024 Aug 28;12(9).
- Ockert LE, McLennan EA, Fox S, Belov K, Hogg CJ. Characterising the Tasmanian devil (Sarcophilus harrisii) pouch microbiome in lactating and non-lactating females. Sci Rep 2024 Jul 2;14(1):15188.
- Kitano H, Kitagawa H, Tadera K, Saito K, Kohada Y, Takemoto K, Kobatake K, Sekino Y, Hieda K, Ohge H, Hinata N. First reported human case of isolation of Vagococcus fluvialis from the urine of a former zoo clerk in Japan: a case report. BMC Infect Dis 2024 Mar 21;24(1):341.
- Chen Q, Tan S, Long S, Wang K, Liu Q. Vagococcus fluvialis isolation from the urine of a bladder cancer patient: a case report. BMC Infect Dis 2024 Feb 26;24(1):261.
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