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Home / Equine Research Bank / J Tissue Eng Regen Med / Characterization and potential applications of progenitor-li...
Journal of tissue engineering and regenerative medicine2011; 6(8); 622-635; doi: 10.1002/term.465

Characterization and potential applications of progenitor-like cells isolated from horse amniotic membrane.

Abstract: The aim of this work was to isolate, for the first time, progenitor-like cells from the epithelial (AECs) and mesenchymal (AMCs) portions of the horse amniotic membrane, and to define the biological properties of these cells. AECs displayed polygonal epithelial morphology, while AMCs were fibroblast-like. Usually, six to eight passages were reached before proliferation decreased, with 13.08 and 26.5 cell population doublings attained after 31 days for AECs and AMCs, respectively. Immunocytochemical studies performed at passage 3 (P3) showed that both cell populations were positive for the expression of specific embryonic markers (TRA-1-60, SSEA-3, SSEA-4 and Oct-4). Meanwhile, RT-PCR performed at P1 and P5 showed expression of mesenchymal stem/stromal cell markers (CD29, CD105, CD44 and CD166) with negativity for CD34 at P1, although this marker began to be expressed by P5. The cells also expressed MHC-I at both P1 and P5, but lacked MHC-II expression at P1. Both AECs and AMCs demonstrated high plasticity, differentiating in vitro toward the osteogenic, adipogenic, chondrogenic and neurogenic lineages. Equine amnion-derived cells could also be frozen and recovered without loss of their functional integrity in terms of morphology, presence of specific stemness markers and differentiation ability, although the renewal capacity was lower than that observed for freshly isolated cells. To investigate potential therapeutic effects and cell tolerance in vivo, horse amnion-derived cells were allogeneically injected into three horses with tendon injuries, resulting in a quick reduction in tendon size and ultrasonographic cross-sectional area measurements. These results suggest that horse amnion-derived cells may be useful for cell therapy applications.
Copyright © 2011 John Wiley & Sons, Ltd.
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Publication Date: 2011-09-22 PubMed ID: 21948689DOI: 10.1002/term.465Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on isolating cells with regenerative properties from the amniotic membrane of horses, studying these cells’ characteristics, and assessing their potential for medical applications, especially in healing tendon injuries.

Isolation and Characterization of Amnion-derived cells

  • The research originated from the need to better understand cells in the amniotic membranes of horses, particularly epithelial cells (AECs) and mesenchymal cells (AMCs), both of which have progenitor-like properties.
  • AECs had a polygonal epithelial structure, while AMCs were found to be more fibroblast-like. The research shows that the cells could usually go through six to eight rounds of division before they stopped proliferating.
  • The study established that after 31 days, the AEC population could double 13.08 times, while the AMC population could double 26.5 times.

Study of Cell Markers

  • Immunocytochemical studies were conducted to investigate the presence of specific embryonic markers (TRA-1-60, SSEA-3, SSEA-4, and Oct-4) in both cell populations. The study found these markers to be present.
  • RT-PCR done at the first and fifth passages showed expression of mesenchymal stem/stromal cell markers (CD29, CD105, CD44, and CD166).
  • CD34 was not present at the first passage but began to appear by the fifth. The cells also displayed MHC-I at both passages but lacked MHC-II expression at the first passage.

Pluripotency and Differentiation Abilities

  • Both AECs and AMCs displayed high plasticity, demonstrating the ability to differentiate into various lineages – osteogenic, adipogenic, chondrogenic, and neurogenic.
  • Moreover, these equine amnion-derived cells could be frozen and later recovered without losing the characteristics indicating their stemness and the ability to differentiate. But, they had a lower renewability as compared to freshly isolated cells.

Therapeutic Applications of the Cells

  • To test the therapeutic potential of these cells, they were injected into three horses with tendon injuries.
  • The results showed a quick reduction in the tendon size and ultrasonographic cross-sectional area measurements, indicating a potential healing effect.
  • This suggests that horse amnion-derived cells may be useful for cell therapy applications, particularly in facilitating recovery from tendon injuries.

Cite This Article

APA
Lange-Consiglio A, Corradetti B, Bizzaro D, Magatti M, Ressel L, Tassan S, Parolini O, Cremonesi F. (2011). Characterization and potential applications of progenitor-like cells isolated from horse amniotic membrane. J Tissue Eng Regen Med, 6(8), 622-635. https://doi.org/10.1002/term.465

Publication

Journal of tissue engineering and regenerative medicine
ISSN: 1932-7005
NlmUniqueID: 101308490
Country: England
Language: English
Volume: 6
Issue: 8
Pages: 622-635

Researcher Affiliations

Lange-Consiglio, A
  • Università degli Studi di Milano, Large Animal Hospital, Reproduction Unit, Lodi, Italy.
Corradetti, B
    Bizzaro, D
      Magatti, M
        Ressel, L
          Tassan, S
            Parolini, O
              Cremonesi, F

                MeSH Terms

                • Amnion / cytology
                • Animals
                • Biological Assay
                • Cell Differentiation
                • Cell Proliferation
                • Cell Separation / methods
                • Cell Shape
                • Colony-Forming Units Assay
                • Epithelial Cells / cytology
                • Female
                • Horses
                • Immunohistochemistry
                • Mesenchymal Stem Cells / cytology
                • Multipotent Stem Cells / cytology
                • Reverse Transcriptase Polymerase Chain Reaction
                • Rupture
                • Staining and Labeling
                • Stem Cell Transplantation
                • Stem Cells / cytology
                • Tendons / diagnostic imaging
                • Tendons / pathology
                • Ultrasonography

                Citations

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