Characterization of a 2.6 kbp variable region within a class 1 integron found in an Acinetobacter baumannii strain isolated from a horse.
Abstract: A complete gene cassette contained in a class 1 integron from a multidrug-resistant (MDR) isolate of Acinetobacter baumannii cultured from a horse was characterized by molecular methods. Methods: Template genomic DNA purified from the A. baumannii isolate was investigated by PCR. A gene cassette-associated amplicon was detected and completely characterized. Results: A 2.6 kbp DNA fragment containing four gene cassettes was amplified from the MDR A. baumannii isolate. Sequence analysis showed it was similar to sequences recently reported in Klebsiella pneumoniae, Serratia marcescens and an Escherichia coli plasmid p1658/97 which conferred aminoglycoside resistance. Aminoglycoside resistance-encoding genes aacC1 and aadA1 were located within the 2.6 kbp amplicon, separated by two open reading frames (ORFs) coding for unknown products. This cassette structure (and some variants) was identified in unrelated Acinetobacter spp. from human sources, based on sequence comparisons of the current databases. Conclusions: Identification of a complete class 1 integron in an equine isolate of A. baumannii suggests that the screening of isolates from animals for these elements should be considered, as this information could influence the selection of chemotherapeutic agents.
Publication Date: 2005-01-28 PubMed ID: 15681585DOI: 10.1093/jac/dkh543Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The researchers have characterized a certain gene cassette in a multidrug-resistant strain of Acinetobacter baumannii that originated from a horse. This finding suggests that testing samples from animal sources for similar elements might become a useful tool that could help in determining the choice of therapeutic drugs.
Research Methods
- The researchers examined a multidrug-resistant isolate of Acinetobacter baumannii that was derived from a horse.
- The genomic DNA from the A. baumannii isolate served as the template for the investigation.
- Through PCR or Polymerase Chain Reaction, a specific gene cassette-associated amplicon was detected and thoroughly studied.
Research Findings
- The team was able to amplify a 2.6 kbp DNA fragment that held four gene cassettes from the multidrug-resistant A. baumannii isolate.
- After examining the sequence, it was found to be similar to those reported earlier in Klebsiella pneumoniae, Serratia marcescens, and an Escherichia coli plasmid p1658/97 that is known to confer resistance to aminoglycosides.
- Aminoglycoside resistance-encoding genes, namely aacC1 and aadA1, were discovered within the 2.6 kbp amplicon. They are separated by two empty reading frames (ORFs) that code for unidentified products.
- This structural formation of cassettes and some variants was recognised in unrelated Acinetobacter species originating from human sources. This was based on comparisons with the sequence from current databases.
Conclusion
- The study successfully identified a full class 1 integron in an equine A. baumannii isolate. This demonstrates that the screening of isolates obtained from animals for such elements needs to be taken into account.
- The gathered information might potentially influence the choice of chemotherapeutic agents by providing deeper insights into pathogen resistance patterns.
Cite This Article
APA
Abbott Y, O'Mahony R, Leonard N, Quinn PJ, van der Reijden T, Dijkshoorn L, Fanning S.
(2005).
Characterization of a 2.6 kbp variable region within a class 1 integron found in an Acinetobacter baumannii strain isolated from a horse.
J Antimicrob Chemother, 55(3), 367-370.
https://doi.org/10.1093/jac/dkh543 Publication
Researcher Affiliations
- Department of Veterinary Microbiology & Parasitology, Faculty of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
MeSH Terms
- Acinetobacter baumannii / drug effects
- Acinetobacter baumannii / genetics
- Amino Acid Sequence
- Animals
- Drug Resistance, Multiple, Bacterial / genetics
- Horses / microbiology
- Integrons
- Molecular Sequence Data
Citations
This article has been cited 8 times.- Mitchell SW, Moran RA, Elbourne LDH, Chapman B, Bull M, Muscatello G, Coleman NV. Impacts of Domestication and Veterinary Treatment on Mobile Genetic Elements and Resistance Genes in Equine Fecal Bacteria. Appl Environ Microbiol 2023 Mar 29;89(3):e0159022.
- Argudín MA, Deplano A, Meghraoui A, Dodémont M, Heinrichs A, Denis O, Nonhoff C, Roisin S. Bacteria from Animals as a Pool of Antimicrobial Resistance Genes. Antibiotics (Basel) 2017 Jun 6;6(2).
- Zordan S, Prenger-Berninghoff E, Weiss R, van der Reijden T, van den Broek P, Baljer G, Dijkshoorn L. Multidrug-resistant Acinetobacter baumannii in veterinary clinics, Germany. Emerg Infect Dis 2011 Sep;17(9):1751-4.
- Endimiani A, Hujer KM, Hujer AM, Bertschy I, Rossano A, Koch C, Gerber V, Francey T, Bonomo RA, Perreten V. Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data. J Antimicrob Chemother 2011 Oct;66(10):2248-54.
- Muller MG, George AR, Walochnik J. Acinetobacter baumannii in Localised Cutaneous Mycobacteriosis in Falcons. Vet Med Int 2010 Sep 5;2010.
- Valenzuela JK, Thomas L, Partridge SR, van der Reijden T, Dijkshoorn L, Iredell J. Horizontal gene transfer in a polyclonal outbreak of carbapenem-resistant Acinetobacter baumannii. J Clin Microbiol 2007 Feb;45(2):453-60.
- Hujer KM, Hujer AM, Hulten EA, Bajaksouzian S, Adams JM, Donskey CJ, Ecker DJ, Massire C, Eshoo MW, Sampath R, Thomson JM, Rather PN, Craft DW, Fishbain JT, Ewell AJ, Jacobs MR, Paterson DL, Bonomo RA. Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. Antimicrob Agents Chemother 2006 Dec;50(12):4114-23.
- Lupo A, Haenni M, Madec JY. Antimicrobial Resistance in Acinetobacter spp. and Pseudomonas spp. Microbiol Spectr 2018 Jun;6(3).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
