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International journal of systematic bacteriology1999; 49 Pt 4; 1381-1385; doi: 10.1099/00207713-49-4-1381

Characterization of a novel gram-positive, catalase-negative coccus from horses: description of Eremococcus coleocola gen. nov., sp. nov.

Abstract: Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic coccus originating from the reproductive tract of horses were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the two strains constitute a new subline within the lactic-acid group of bacteria, close to, but distinct from, Abiotrophia defectiva, Globicatella sanguinis and close relatives. The unknown bacterium was readily distinguished from other described Gram-positive, catalase-negative cocci by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Eremococcus coleocola gen. nov., sp. nov. The type strain of Eremococcus coleocola is CCUG 38207T.
Publication Date: 1999-11-11 PubMed ID: 10555316DOI: 10.1099/00207713-49-4-1381Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research study identifies a new type of bacterium originating from the reproductive tract of horses. Using rest tests and gene sequencing, the researchers were able to distinguish this bacterium from others, and have named it Eremococcus coleocola.

Characterization of a Novel Bacterium

  • This research article presents their study on identifying and characterizing a novel bacterium found in the reproductive tract of horses. After conducting a series of tests and observations, it was confirmed that the bacterium is new.
  • This bacterium is Gram-positive, meaning it takes up the violet Gram staining due to a thick layer of peptidoglycan in its cell wall, and catalase-negative which means it lacks the enzyme catalase.
  • Being a facultative anaerobe, the bacterium is capable of growing with or without oxygen. In other words, it can adjust its metabolism according to the environmental conditions.

Comparative 16S rRNA gene sequencing studies

  • This procedure is a popular tool used for identifying and classifying bacteria. By comparing the 16S rRNA gene sequences, researchers were able to confirm that the two strains of this bacterium were new and did not fit into any existing bacterial species.
  • Despite finding notable similarities with the lactic-acid group of bacteria, the unknown bacterium was distinct from Abiotrophia defectiva, Globicatella sanguinis and close relatives.

Classifying the New Bacterium

  • After distinguishing the unknown bacterium from other described Gram-positive, catalase-negative cocci through biochemical tests and analysis of the whole-cell proteins, the researchers proposed that this bacterium be classified as a new species and genus – Eremococcus coleocola.
  • The identification and classification of this bacterium doesn’t just represent a significant contribution to the taxonomic field, but could also have potential implications in the identification and treatment of horse diseases.

The Type Strain Eremococcus coleocola CCUG 38207T

  • The type strain of a species is the strain that was originally used to describe and classify the species. In this case, it is CCUG 38207T for Eremococcus coleocola.
  • This identification serves as the ultimate reference point for the newly discovered species and it is deposited in a culture collection to enable other researchers to access and study this microorganism.

Cite This Article

APA
Collins MD, Rodriguez Jovita M, Lawson PA, Falsen E, Foster G. (1999). Characterization of a novel gram-positive, catalase-negative coccus from horses: description of Eremococcus coleocola gen. nov., sp. nov. Int J Syst Bacteriol, 49 Pt 4, 1381-1385. https://doi.org/10.1099/00207713-49-4-1381

Publication

ISSN: 0020-7713
NlmUniqueID: 0042143
Country: England
Language: English
Volume: 49 Pt 4
Pages: 1381-1385

Researcher Affiliations

Collins, M D
  • Department of Food Science and Technology, University of Reading, UK. David.Collins@bbsrc.ac.uk
Rodriguez Jovita, M
    Lawson, P A
      Falsen, E
        Foster, G

          MeSH Terms

          • Animals
          • Bacterial Typing Techniques
          • Catalase / metabolism
          • Female
          • Genes, rRNA
          • Gram-Positive Bacterial Infections / microbiology
          • Gram-Positive Bacterial Infections / veterinary
          • Gram-Positive Cocci / classification
          • Gram-Positive Cocci / genetics
          • Gram-Positive Cocci / isolation & purification
          • Horse Diseases / microbiology
          • Horses
          • Molecular Sequence Data
          • Phylogeny
          • RNA, Bacterial / genetics
          • RNA, Ribosomal, 16S / genetics
          • Sequence Analysis, DNA
          • Vaginal Discharge / microbiology
          • Vaginal Discharge / veterinary

          Citations

          This article has been cited 5 times.
          1. Doyle CJ, Gleeson D, O'Toole PW, Cotter PD. Impacts of Seasonal Housing and Teat Preparation on Raw Milk Microbiota: a High-Throughput Sequencing Study. Appl Environ Microbiol 2017 Jan 15;83(2).
            doi: 10.1128/AEM.02694-16pubmed: 27815277google scholar: lookup
          2. Iraola G, Pérez R, Naya H, Paolicchi F, Pastor E, Valenzuela S, Calleros L, Velilla A, Hernández M, Morsella C. Genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus Campylobacter. Genome Biol Evol 2014 Sep 4;6(9):2392-405.
            doi: 10.1093/gbe/evu195pubmed: 25193310google scholar: lookup
          3. Vela AI, Fernández E, las Heras A, Lawson PA, Domínguez L, Collins MD, Fernandez-Garayzabal JF. Meningoencephalitis associated with Globicatella sanguinis infection in lambs. J Clin Microbiol 2000 Nov;38(11):4254-5.
          4. Manavalan S, Pradeep D, Dharmalingam D, Semalaiyappan J, Sivarasan T, Venkatesan S, Thirumal S, Kuttiatt VS. Comparative analysis of skin microbiome of patients with filarial lymphedema and healthy individuals. PLoS One 2025;20(7):e0325380.
            doi: 10.1371/journal.pone.0325380pubmed: 40601568google scholar: lookup
          5. Zhou G, Gao L, Fang BZ, Wang YS, Tao HB, Wen X, Wang Q, Huang XM, Shi QS, Li WJ, Xie XB. Fundicoccus culcitae sp. nov., a novel potential bacteriocin producing bacterium isolated from a spoiled eye mask. Antonie Van Leeuwenhoek 2023 Nov;116(11):1185-1195.
            doi: 10.1007/s10482-023-01866-5pubmed: 37704902google scholar: lookup