FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Archives of virology1991; 119(1-2); 95-109; doi: 10.1007/BF01314326

Characterization of BPV-like DNA in equine sarcoids.

Abstract: The DNA from equine sarcoid samples from New York State and Switzerland was isolated and probed with bovine papillomavirus type 1 (BPV-1) to determine if BPV genomes were present. Twelve of 13 sarcoids from New York State and 17/20 sarcoids from Switzerland contained DNA that hybridized to the BPV-1 probe. Restriction enzyme analysis of the positive samples demonstrated restriction fragment profiles characteristic of BPV-1 in 22 sarcoids and restriction fragment profiles characteristic of bovine papillomavirus type 2 (BPV-2) in 7 sarcoids. In addition, three tissues histologically diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma contained BPV-like DNA. Tissues with BPV-1-like and BPV-2-like DNA contained an average of 285.7 (21 to 808) and 125.8 (2 to 762) BPV-like genomes per cell, respectively. Minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids. BPV-like DNA was not detected in lymphocyte DNA from sarcoid-affected horses. These results confirm previous observations and support the hypothesis that bovine papillomavirus, or a very similar virus, is linked to the cause of equine sarcoid.
Get Full Text
Publication Date: 1991-01-01 PubMed ID: 1650553DOI: 10.1007/BF01314326Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study conducted on equine sarcoids to uncover if they contain Bovine Papillomavirus (BPV) genomes, back up previous observations, and strengthen the theory that equine sarcoid has a link to a virus similar to Bovine Papillomavirus.

Research Overview

The research was primarily conducted to:

  • Probe DNA isolated from equine sarcoid samples with the bovine papillomavirus type 1(BPV-1) to see if BPV genomes were found in these samples.
  • Analyze the samples that positively reacted to the BPV-1 probe using restriction enzyme and determine their restriction fragment profiles.

Methodology

  • DNA was isolated from equine sarcoids collected from New York State and Switzerland.
  • The isolated DNA was then probed with bovine papillomavirus type 1 (BPV-1).
  • Positive samples underwent restriction enzyme analysis to reveal their restriction fragment profiles.

Findings

The main findings of the research are as follows:

  • Of the investigated sarcoids, twelve out of thirteen from New York State and seventeen out of twenty from Switzerland contained DNA that had a reaction to the BPV-1 probe.
  • 22 sarcoids portrayed restriction fragment profiles typical of bovine papillomavirus type one (BPV-1).
  • 7 sarcoids showed restriction fragment profiles that were typical of bovine papillomavirus type 2(BPV-2).
  • BPV-like DNA was also found in tissues diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma.
  • There were minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids.
  • BPV-like DNA was not detected in lymphocyte DNA from horses affected with sarcoids.

Conclusion

The outcome of this research underscores previous observations and provides stronger credence to the hypothesis that bovine papillomavirus, or a virus closely related to it, is tied to the cause of equine sarcoid. The exact nature and implications of this relationship continue to be a subject of ongoing research.

Cite This Article

APA
Angelos JA, Marti E, Lazary S, Carmichael LE. (1991). Characterization of BPV-like DNA in equine sarcoids. Arch Virol, 119(1-2), 95-109. https://doi.org/10.1007/BF01314326

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 119
Issue: 1-2
Pages: 95-109

Researcher Affiliations

Angelos, J A
  • James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca.
Marti, E
    Lazary, S
      Carmichael, L E

        MeSH Terms

        • Animals
        • Blotting, Southern / veterinary
        • Bovine papillomavirus 1 / genetics
        • Bovine papillomavirus 1 / isolation & purification
        • DNA Probes
        • DNA Restriction Enzymes
        • DNA, Viral / analysis
        • Horse Diseases / microbiology
        • Horses
        • Sarcoidosis / microbiology
        • Sarcoidosis / veterinary
        • Skin Diseases / microbiology
        • Skin Diseases / veterinary

        References

        This article includes 25 references
        1. Jarrett WF, Campo MS, Blaxter ML, O'Neil BW, Laird HM, Moar MH, Sartirana ML. Alimentary fibropapilloma in cattle: a spontaneous tumor, nonpermissive for papillomavirus replication.. J Natl Cancer Inst 1984 Aug;73(2):499-504.
          pubmed: 6087008doi: 10.1093/jnci/73.2.499google scholar: lookup
        2. Meinkoth J, Wahl G. Hybridization of nucleic acids immobilized on solid supports.. Anal Biochem 1984 May 1;138(2):267-84.
          pubmed: 6204550doi: 10.1016/0003-2697(84)90808-xgoogle scholar: lookup
        3. OLSON C Jr, COOK RH. Cutaneous sarcoma-like lesions of the horse caused by the agent of bovine papilloma.. Proc Soc Exp Biol Med 1951 Jun;77(2):281-4.
          pubmed: 14854020doi: 10.3181/00379727-77-18750google scholar: lookup
        4. Pascoe RR, Summers PM. Clinical survey of tumours and tumour-like lesions in horses in south east Queensland.. Equine Vet J 1981 Oct;13(4):235-9.
          pubmed: 6459231doi: 10.1111/j.2042-3306.1981.tb03504.xgoogle scholar: lookup
        5. Lancaster WD. Physical maps of bovine papillomavirus type 1 and type 2 genomes.. J Virol 1979 Nov;32(2):684-7.
          pubmed: 228086doi: 10.1128/JVI.32.2.684-687.1979google scholar: lookup
        6. Lancaster WD, Olson C, Meinke W. Quantitation of bovine papilloma viral DNA in viral-induced tumors.. J Virol 1976 Mar;17(3):824-31.
          pubmed: 176447doi: 10.1128/JVI.17.3.824-831.1976google scholar: lookup
        7. Lancaster WD, Theilen GH, Olson C. Hybridization of bovine papilloma virus type 1 and type 2 DNA to DNA from virus-induced hamster tumors and naturally occurring equine tumors.. Intervirology 1979;11(4):227-33.
          pubmed: 218902doi: 10.1159/000149038google scholar: lookup
        8. Ragland WL, Keown GH, Gorham JR. An epizootic of equine sarcoid.. Nature 1966 Jun 25;210(5043):1399.
          pubmed: 6007121doi: 10.1038/2101399a0google scholar: lookup
        9. Campo MS, Moar MH, Laird HM, Jarrett WF. Molecular heterogeneity and lesion site specificity of cutaneous bovine papillomaviruses.. Virology 1981 Aug;113(1):323-35.
          pubmed: 6267786doi: 10.1016/0042-6822(81)90159-8google scholar: lookup
        10. Amtmann E, Müller H, Sauer G. Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA.. J Virol 1980 Sep;35(3):962-4.
          pubmed: 6252350doi: 10.1128/JVI.35.3.962-964.1980google scholar: lookup
        11. SEGRE D, OLSON C Jr, HOERLEIN AB. Neutralization of bovine papilloma virus with serums from cattle and horses with experimental papillomas.. Am J Vet Res 1955 Oct;16(61 Part 1):517-20.
          pubmed: 13259027
        12. Lancaster WD, Olson C, Meinke W. Bovine papilloma virus: presence of virus-specific DNA sequences in naturally occurring equine tumors.. Proc Natl Acad Sci U S A 1977 Feb;74(2):524-8.
          pubmed: 191813doi: 10.1073/pnas.74.2.524google scholar: lookup
        13. Ragland WL, Spencer GR. Attempts to relate bovine papilloma virus to the cause of equine sarcoid: equidae inoculated intradermally with bovine papilloma virus.. Am J Vet Res 1969 May;30(5):743-52.
          pubmed: 5813668
        14. Angelos J, Oppenheim Y, Rebhun W, Mohammed H, Antczak DF. Evaluation of breed as a risk factor for sarcoid and uveitis in horses.. Anim Genet 1988;19(4):417-25.
          pubmed: 3232865doi: 10.1111/j.1365-2052.1988.tb00833.xgoogle scholar: lookup
        15. Evans CA, Ito Y. Antitumor immunity in the Shope papilloma-carcinoma complex of rabbits. 3. Response to reinfection with viral nucleic acid.. J Natl Cancer Inst 1966 Jun;36(6):1161-6.
          pubmed: 5944528
        16. Amtmann E, Volm M, Wayss K. Tumour induction in the rodent Mastomys natalensis by activation of endogenous papilloma virus genomes.. Nature 1984 Mar 15-21;308(5956):291-2.
          pubmed: 6322013doi: 10.1038/308291a0google scholar: lookup
        17. Meredith D, Elser AH, Wolf B, Soma LR, Donawick WJ, Lazary S. Equine leukocyte antigens: relationships with sarcoid tumors and laminitis in two pure breeds.. Immunogenetics 1986;23(4):221-5.
          pubmed: 3699852doi: 10.1007/BF00373016google scholar: lookup
        18. O'Banion MK, Reichmann ME, Sundberg JP. Cloning and characterization of an equine cutaneous papillomavirus.. Virology 1986 Jul 15;152(1):100-9.
          pubmed: 3012863doi: 10.1016/0042-6822(86)90375-2google scholar: lookup
        19. Lazary S, Gerber H, Glatt PA, Straub R. Equine leucocyte antigens in sarcoid-affected horses.. Equine Vet J 1985 Jul;17(4):283-6.
          pubmed: 3865769doi: 10.1111/j.2042-3306.1985.tb02498.xgoogle scholar: lookup
        20. Bloom ME, Race RE, Aasted B, Wolfinbarger JB. Analysis of Aleutian disease virus infection in vitro and in vivo: demonstration of Aleutian disease virus DNA in tissues of infected mink.. J Virol 1985 Sep;55(3):696-703.
          pubmed: 3894686doi: 10.1128/JVI.55.3.696-703.1985google scholar: lookup
        21. Pfister H. Biology and biochemistry of papillomaviruses.. Rev Physiol Biochem Pharmacol 1984;99:111-81.
          pubmed: 6320328doi: 10.1007/BFb0027716google scholar: lookup
        22. Trenfield K, Spradbrow PB, Vanselow B. Sequences of papillomavirus DNA in equine sarcoids.. Equine Vet J 1985 Nov;17(6):449-52.
          pubmed: 3000762doi: 10.1111/j.2042-3306.1985.tb02553.xgoogle scholar: lookup
        23. Ragland WL, Spencer GR. Attempts to relate bovine papilloma virus to the cause of equine sarcoid: immunity to bovine papilloma virus.. Am J Vet Res 1968 Jul;29(7):1363-6.
          pubmed: 4298397
        24. Danos O, Giri I, Thierry F, Yaniv M. Papillomavirus genomes: sequences and consequences.. J Invest Dermatol 1984 Jul;83(1 Suppl):7s-11s.
          pubmed: 6330226doi: 10.1111/1523-1747.ep12281115google scholar: lookup
        25. Wigler M, Sweet R, Sim GK, Wold B, Pellicer A, Lacy E, Maniatis T, Silverstein S, Axel R. Transformation of mammalian cells with genes from procaryotes and eucaryotes.. Cell 1979 Apr;16(4):777-85.
          pubmed: 222468doi: 10.1016/0092-8674(79)90093-xgoogle scholar: lookup

        Citations

        This article has been cited 11 times.
        1. Gysens L, Vanmechelen B, Haspeslagh M, Maes P, Martens A. New approach for genomic characterisation of equine sarcoid-derived BPV-1/-2 using nanopore-based sequencing. Virol J 2022 Jan 6;19(1):8.
          doi: 10.1186/s12985-021-01735-5pubmed: 34991633google scholar: lookup
        2. Martano M, Altamura G, Power K, Restucci B, Carella F, Borzacchiello G, Maiolino P. Evaluation of Hypoxia-Inducible Factor-1 Alpha (HIF-1α) in Equine Sarcoid: An Immunohistochemical and Biochemical Study. Pathogens 2020 Jan 14;9(1).
          doi: 10.3390/pathogens9010058pubmed: 31947661google scholar: lookup
        3. Araldi RP, Assaf SMR, Carvalho RF, Carvalho MACR, Souza JM, Magnelli RF, Módolo DG, Roperto FP, Stocco RC, Beçak W. Papillomaviruses: a systematic review. Genet Mol Biol 2017 Jan-Mar;40(1):1-21.
          doi: 10.1590/1678-4685-GMB-2016-0128pubmed: 28212457google scholar: lookup
        4. Egawa N, Egawa K, Griffin H, Doorbar J. Human Papillomaviruses; Epithelial Tropisms, and the Development of Neoplasia. Viruses 2015 Jul 16;7(7):3863-90.
          doi: 10.3390/v7072802pubmed: 26193301google scholar: lookup
        5. Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids. J Clin Microbiol 2013 Jul;51(7):2167-71.
          doi: 10.1128/JCM.00371-13pubmed: 23637294google scholar: lookup
        6. Wobeser BK, Davies JL, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL. Epidemiology of equine sarcoids in horses in western Canada. Can Vet J 2010 Oct;51(10):1103-8.
          pubmed: 21197201
        7. Yuan ZQ, Nicolson L, Marchetti B, Gault EA, Campo MS, Nasir L. Transcriptional changes induced by bovine papillomavirus type 1 in equine fibroblasts. J Virol 2008 Jul;82(13):6481-91.
          doi: 10.1128/JVI.00429-08pubmed: 18434409google scholar: lookup
        8. Postey RC, Appleyard GD, Kidney BA. Evaluation of equine papillomas, aural plaques, and sarcoids for the presence of Equine papillomavirus DNA and Papillomavirus antigen. Can J Vet Res 2007 Jan;71(1):28-33.
          pubmed: 17193879
        9. Otten N, von Tscharner C, Lazary S, Antczak DF, Gerber H. DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing. Arch Virol 1993;132(1-2):121-31.
          doi: 10.1007/BF01309847pubmed: 8394687google scholar: lookup
        10. Sobhy NM, Refaai W, Kumar R, Bottros Youssef CR, Goyal SM. Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt. Vet Med Int 2025;2025:9773642.
          doi: 10.1155/vmi/9773642pubmed: 39803352google scholar: lookup
        11. Karalus W, Subharat S, Orbell G, Vaatstra B, Munday JS. Equine sarcoids: A clinicopathologic study of 49 cases, with mitotic count and clinical type predictive of recurrence. Vet Pathol 2024 May;61(3):357-365.
          doi: 10.1177/03009858231209408pubmed: 37937724google scholar: lookup
        Subscribe to our newsletter
        Join 99,850 horse owners like you who receive our email updates on horse health and nutrition.