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Home / Equine Research Bank / Arch Virol / Characterization of BPV-like DNA in equine sarcoids.
Archives of virology1991; 119(1-2); 95-109; doi: 10.1007/BF01314326

Characterization of BPV-like DNA in equine sarcoids.

Abstract: The DNA from equine sarcoid samples from New York State and Switzerland was isolated and probed with bovine papillomavirus type 1 (BPV-1) to determine if BPV genomes were present. Twelve of 13 sarcoids from New York State and 17/20 sarcoids from Switzerland contained DNA that hybridized to the BPV-1 probe. Restriction enzyme analysis of the positive samples demonstrated restriction fragment profiles characteristic of BPV-1 in 22 sarcoids and restriction fragment profiles characteristic of bovine papillomavirus type 2 (BPV-2) in 7 sarcoids. In addition, three tissues histologically diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma contained BPV-like DNA. Tissues with BPV-1-like and BPV-2-like DNA contained an average of 285.7 (21 to 808) and 125.8 (2 to 762) BPV-like genomes per cell, respectively. Minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids. BPV-like DNA was not detected in lymphocyte DNA from sarcoid-affected horses. These results confirm previous observations and support the hypothesis that bovine papillomavirus, or a very similar virus, is linked to the cause of equine sarcoid.
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Publication Date: 1991-01-01 PubMed ID: 1650553DOI: 10.1007/BF01314326Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study conducted on equine sarcoids to uncover if they contain Bovine Papillomavirus (BPV) genomes, back up previous observations, and strengthen the theory that equine sarcoid has a link to a virus similar to Bovine Papillomavirus.

Research Overview

The research was primarily conducted to:

  • Probe DNA isolated from equine sarcoid samples with the bovine papillomavirus type 1(BPV-1) to see if BPV genomes were found in these samples.
  • Analyze the samples that positively reacted to the BPV-1 probe using restriction enzyme and determine their restriction fragment profiles.

Methodology

  • DNA was isolated from equine sarcoids collected from New York State and Switzerland.
  • The isolated DNA was then probed with bovine papillomavirus type 1 (BPV-1).
  • Positive samples underwent restriction enzyme analysis to reveal their restriction fragment profiles.

Findings

The main findings of the research are as follows:

  • Of the investigated sarcoids, twelve out of thirteen from New York State and seventeen out of twenty from Switzerland contained DNA that had a reaction to the BPV-1 probe.
  • 22 sarcoids portrayed restriction fragment profiles typical of bovine papillomavirus type one (BPV-1).
  • 7 sarcoids showed restriction fragment profiles that were typical of bovine papillomavirus type 2(BPV-2).
  • BPV-like DNA was also found in tissues diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma.
  • There were minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids.
  • BPV-like DNA was not detected in lymphocyte DNA from horses affected with sarcoids.

Conclusion

The outcome of this research underscores previous observations and provides stronger credence to the hypothesis that bovine papillomavirus, or a virus closely related to it, is tied to the cause of equine sarcoid. The exact nature and implications of this relationship continue to be a subject of ongoing research.

Cite This Article

APA
Angelos JA, Marti E, Lazary S, Carmichael LE. (1991). Characterization of BPV-like DNA in equine sarcoids. Arch Virol, 119(1-2), 95-109. https://doi.org/10.1007/BF01314326

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 119
Issue: 1-2
Pages: 95-109

Researcher Affiliations

Angelos, J A
  • James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca.
Marti, E
    Lazary, S
      Carmichael, L E

        MeSH Terms

        • Animals
        • Blotting, Southern / veterinary
        • Bovine papillomavirus 1 / genetics
        • Bovine papillomavirus 1 / isolation & purification
        • DNA Probes
        • DNA Restriction Enzymes
        • DNA, Viral / analysis
        • Horse Diseases / microbiology
        • Horses
        • Sarcoidosis / microbiology
        • Sarcoidosis / veterinary
        • Skin Diseases / microbiology
        • Skin Diseases / veterinary

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        Citations

        This article has been cited 9 times.
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