Characterization of equine arteritis virus particles and demonstration of their hemolytic activity.
Abstract: Equine arteritis virus (EAV), a member of the newly established family Arteriviridae, is a small, positive-stranded RNA virus. It carries two protein complexes in its envelope, gp5/M and the recently described gp2b/gp3/gp4 complex. We report here on several basic features of EAV replication in cell culture and on the protein composition of virus particles. We have also characterized gp2b, gp3, and gp4 expressed using a baculovirus system in insect cells. Finally, we provide evidence that EAV possess hemagglutinating and hemolytic activity. The hemolysis assay might be useful for determining which of the surface proteins carries the receptor-binding and membrane fusion activity of EAV.
Publication Date: 2008-02-04 PubMed ID: 18219439PubMed Central: PMC7086761DOI: 10.1007/s00705-007-1094-yGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study focuses on exploring the basic attributes of Equine arteritis virus (EAV) replication in cell culture and its protein composition, and finding evidence of its hemagglutinating and hemolytic activity which could potentially help understand which surface protein accounts for the receptor-binding and membrane fusion activity of EAV.
Understanding Equine arteritis virus (EAV)
- EAV is a small, positive-stranded RNA virus and belongs to the newly established family Arteriviridae.
- It contains two protein complexes in its envelope: gp5/M and gp2b/gp3/gp4 complex.
Characterizing EAV replication and protein composition
- This research focuses on discovering the basic features associated with EAV replication in a controlled cell culture and analyzing the protein composition of the virus particles.
- The study also characterizes specific proteins such as gp2b, gp3, and gp4 which were expressed using a baculovirus system in insect cells.
Hemolytic Activity of EAV
- The research implies that EAV features hemagglutinating and hemolytic activity.
- Hemagglutination refers to clumping of red blood cells while hemolysis refers to the breakdown of red blood cells.
Implication of Hemolytic Activity
- The hemolytic activity of EAV might be used to identify the receptor-binding and membrane fusion activity of EAV.
- This indicates that the surface proteins of EAV could play a crucial role in its binding to cell receptors and subsequent entry into the cell, leading to infection.
- A hemolysis assay could be an applicable experimental setup for determining the functional domain of the virus particle that is responsible for receptor-binding and membrane fusion.
Cite This Article
APA
Veit M, Kabatek A, Tielesch C, Hermann A.
(2008).
Characterization of equine arteritis virus particles and demonstration of their hemolytic activity.
Arch Virol, 153(2), 351-356.
https://doi.org/10.1007/s00705-007-1094-y Publication
Researcher Affiliations
- Department of Immunology and Molecular Biology, Veterinary Faculty, Free University Berlin, Berlin, Germany. mveit@zedat.fu-berlin.de
MeSH Terms
- Animals
- Baculoviridae
- Cell Line
- Chickens
- Cricetinae
- Electrophoresis, Polyacrylamide Gel
- Equartevirus / chemistry
- Equartevirus / pathogenicity
- Equartevirus / physiology
- Erythrocytes / virology
- Hemagglutination
- Hemolysis
- Spodoptera
- Viral Proteins / analysis
- Virus Replication / physiology
References
This article includes 21 references
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