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Home / Equine Research Bank / J Equine Sci / Characterization of isolates of Bordetella bronchiseptica fr...
Journal of equine science2018; 29(1); 25-31; doi: 10.1294/jes.29.25

Characterization of isolates of Bordetella bronchiseptica from horses.

Abstract: is a well-known Gram-negative bacterial pathogen causing a plethora of diseases in different animals. Although its infection has been reported from pigs and dogs in India, no report of B. bronchiseptica from horses is described. We report for the first time, isolation, identification and characterization of strains of B. bronchiseptica from respiratory infection in horses from different states in India. The antimicrobial susceptibility testing showed resistance to penicillins, ceftazidime, and chloramphanicol. The virulence capability of the strains was confirmed by sequencing genes such as adenylate cyclase toxin (cyaA), bordetella virulence gene (bvgA) and by PCR detection of flagellin gene (fla). We demonstrate the involvement of B. bronchiseptica strains in respiratory tract infection in horses in India.
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Publication Date: 2018-03-23 PubMed ID: 29593446PubMed Central: PMC5865067DOI: 10.1294/jes.29.25Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research presents the first documentation of a certain type of bacteria, Bordetella bronchiseptica, causing respiratory infection in horses in India. The study finds that the bacteria are resistant to several antibiotics and verifies the bacteria’s virulence by identifying specific genes.

Research Objectives and Methodology

  • The objective of this research was to isolate, identify, and characterize strains of the bacteria Bordetella bronchiseptica in horses suffering from respiratory infections in various regions of India.
  • This research is significant because Bordetella bronchiseptica, an acknowledged Gram-negative bacterial pathogen causing diseases in various animals, had not been previously reported in horses in India.
  • The research involved antimicrobial susceptibility testing to note the bacteria’s resistance to certain drugs, including penicillins, ceftazidime, and chloramphanicol.

Findings and Analysis

  • The study found that the strains of B. bronchiseptica isolated from the horses showed resistance to some antibiotics. This poses a potential treatment problem, suggesting the need for alternate drugs or treatment methods.
  • Further supporting the strains’ pathogenic capabilities, researchers identified key virulence genes in the bacteria, such as adenylate cyclase toxin (cyaA), bordetella virulence gene (bvgA) and the flagellin gene (fla).
  • The presence of these genes are significant indicators of the bacteria’s capability to cause disease, supporting the researchers’ assertion of B. bronchiseptica’s role in respiratory infections in horses.

Conclusions

  • This research highlights the need for increased surveillance and understanding of B. bronchiseptica infections in horses, particularly given the newly identified antibiotic resistance.
  • The identified virulence genes of B. bronchiseptica could also be potential markers for diagnostic and therapeutic development strategies in equine health.
  • Overall, this study adds to the understanding of B. bronchiseptica as a pathogen in horses, providing insights for better prevention, diagnosis, and treatment of respiratory tract infections in horses.

Cite This Article

APA
Vaid RK, Shanmugasundaram K, Anand T, Bera BC, Tigga M, Dedar R, Riyesh T, Bardwaj S, Virmani N, Tripathi BN, Singh R. (2018). Characterization of isolates of Bordetella bronchiseptica from horses. J Equine Sci, 29(1), 25-31. https://doi.org/10.1294/jes.29.25

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 29
Issue: 1
Pages: 25-31

Researcher Affiliations

Vaid, Rajesh Kumar
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Shanmugasundaram, Karuppusamy
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Anand, Taruna
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Bera, Bidhan Chandra
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Tigga, Mamta
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Dedar, Ramesh
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Riyesh, Thachamvally
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Bardwaj, Shashank
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Virmani, Nitin
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Tripathi, Bhupendra Nath
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.
Singh, Rajkumar
  • Bacteriology Laboratory, National Centre for Veterinary Type Cultures, National Research Centre on Equines, 125 001 Haryana, India.

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Citations

This article has been cited 2 times.
  1. Van Crombrugge E, Vanbeylen E, Van Cleemput J, Van den Broeck W, Laval K, Nauwynck H. Bacterial Toxins from Staphylococcus aureus and Bordetella bronchiseptica Predispose the Horse's Respiratory Tract to Equine Herpesvirus Type 1 Infection. Viruses 2022 Jan 14;14(1).
    doi: 10.3390/v14010149pubmed: 35062352google scholar: lookup
  2. Wang J, Sun S, Chen Y, Chen D, Sang L, Xie X. Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China. Epidemiol Infect 2020 Aug 24;148:e237.
    doi: 10.1017/S0950268820001879pubmed: 32829720google scholar: lookup
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