Characterization of L-lysine transport across equine and porcine jejunal and colonic brush border membrane.
Abstract: In nonruminant herbivores, microbially derived AA could contribute to whole-body AA homeostasis and thus decrease predicted AA requirements. However, postileal capacity of AA uptake is currently unknown. Therefore, to test the hypothesis that Lys is transported across the large colon mucosal apical membrane with capacity similar to that of the small intestinal mucosa in the pony and pig, we examined Lys transport in vitro using brush border membrane vesicles (BBMV). Mucosa was collected from the distal jejunum (DJ) and proximal large colon (PLC) of growing pigs (n = 3) and ponies (n = 4), flash frozen in liquid nitrogen, and stored at -80°C. Jejunal and colonic BBMV were manufactured by Mg(2+) precipitation and used to determine initial rates and kinetics [the maximal transport rate (V(max)) and the Michaelis constant (K(M))] of l-Lys transport into apical epithelia by rapid filtration technique in Na(+)-gradient incubation buffer. Initial rates of total l-Lys uptake did not differ between the PLC and DJ in either the pig or the pony, or between the pony and the pig, at each l-Lys concentration. In the pig, compared with the DJ, l-Lys transport V(max) in the PLC did not differ (121 ± 26 and 180 ± 26 pmol•mg of protein(-1)•s(-1), respectively; P = 0.14) and l-Lys K(M) in the PLC tended to be greater (0.23 ± 0.22 and 0.89 ± 0.22 mM, respectively; P = 0.09). In the pony, compared with the DJ, l-Lys transport V(max) in the PLC was greater (62 ± 25 and 149 ± 25 pmol•mg of protein(-1)•s(-1), respectively; P = 0.04) and l-Lys K(M) in the PLC was greater (0.08 ± 0.22 and 1.05 ± 0.22 mM, respectively; P = 0.02). l-Lysine diffusion was not different between segments; however, total intestinal diffusion was greater (P = 0.03) in the pony than in the pig (115 ± 10 and 73 ± 10 pmol·mg of protein(-1)•s(-1), respectively). These results demonstrate that the large colon is capable of l-Lys transport across the apical epithelial membrane with greater capacity and less affinity than the jejunum, indicating that the large colon may play a significant role in l-Lys absorption and homeostasis in hindgut fermenters.
Publication Date: 2011-11-07 PubMed ID: 22064742DOI: 10.2527/jas.2011-4210Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the transportation of the amino acid L-lysine across the membranes of the intestines of pigs and ponies. The findings show that the large colon is capable of transporting L-lysine with greater capacity and less affinity than the jejunum, suggesting that it may play a significant role in L-lysine absorption and balance in these animals.
Research Methodology
- The study sought to understand how Lysine, an amino acid, is transported across the jejunal and colonic brush border membranes in pigs and ponies. This was evaluated by examining Lysine transport in vitro using brush border membrane vesicles (BBMV).
- The mucosa was collected from the distal jejunum and proximal large colon of three pigs and four ponies. These samples were flash frozen in liquid nitrogen and stored at -80°C. The researchers then used these samples to make jejunal and colonic brush-border membrane vesicles (BBMV) through a process called Mg(2+) precipitation.
- To measure the initial rates and kinetics of L-lysine, the researchers used the rapid filtration technique in Na(+)-gradient incubation buffer.
Research Findings
- The research shows that the initial uptake rates of L-Lysine did not differ between the proximal large colon (PLC) and the distal jejunum (DJ) in pigs or ponies, or between ponies and pigs at each L-Lysine concentration.
- Comparing the pig’s DJ and PLC, L-Lysine transport V(max) did not differ significantly, and L-Lysine K(M), a measure of its affinity, was slightly greater in the PLC.
- However, for the pony, the results differed. The L-Lysine transport V(max) in the PLC was significantly greater, and L-Lysine K(M) in the PLC was also greater.
- L-Lysine diffusion did not vary between segments; but total intestinal diffusion was significantly higher in the pony than in the pig.
Implications of the Study
- These results demonstrate that the large colon, contrary to previous assumptions, is capable of transporting L-Lysine across its cell membranes with a greater capacity and less affinity than the jejunum.
- This implies that the large colon could play a significant role in L-Lysine absorption and balance in hindgut fermenters like pigs and ponies.
Cite This Article
APA
Woodward AD, Fan MZ, Geor RJ, McCutcheon LJ, Taylor NP, Trottier NL.
(2011).
Characterization of L-lysine transport across equine and porcine jejunal and colonic brush border membrane.
J Anim Sci, 90(3), 853-862.
https://doi.org/10.2527/jas.2011-4210 Publication
Researcher Affiliations
- Department of Animal Science, Michigan State University, East Lansing 48824, USA.
MeSH Terms
- Animals
- Biological Transport, Active / physiology
- Colon / physiology
- Horses / physiology
- Jejunum / physiology
- Lysine / metabolism
- Microvilli / physiology
- Swine / physiology
- Time Factors
- Tissue Culture Techniques
Citations
This article has been cited 3 times.- Lewton JR, Woodward AD, Moser RL, Thelen KM, Moeser AJ, Trottier NL, Tempelman RJ, Rozeboom DW. Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on weanling pig growth performance and nutrient digestibility. Transl Anim Sci 2021 Jul;5(3):txab058.
- Mok CH, Urschel KL. Amino acid requirements in horses. Asian-Australas J Anim Sci 2020 May;33(5):679-695.
- Bonnici C, Marchesi MF, Felici M, Ghiselli F, Majer R, Tugnoli B, Gallina G, Piva A, Grilli E. Optimization of Solid Lipid Microcapsule Matrix for Enhanced Release and Bioavailability of L-Lysine in Swine. Animals (Basel) 2025 Jun 19;15(12).
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