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Home / Equine Research Bank / Sci Rep / Characterization of serum and tissue oxytocinase and tissue ...
Scientific reports2023; 13(1); 4616; doi: 10.1038/s41598-023-31540-9

Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare.

Abstract: Oxytocin is a hormone with functions in: reproduction, maternal bonding, milk ejection, and feeding/social behavior, and is reported to be present in a variety of tissues. Our goal is to characterize oxytocin and leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase), a key regulator of oxytocin in mares. We measured serum and tissue LNPEP by ELISA from ovulation (D0) until D21-22 in non-pregnant (n = 5) and pregnant mares (n = 6); and in periparturient and postpartum mares (n = 18). Placenta (n = 7) and homogenized tissue of diestrus mares (n = 6) were evaluated using protein determinations and LNPEP ELISAs. Identification of LNPEP and OXT protein in tissues was also performed via western blot, immunohistochemistry and liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, in situ hybridization was performed for LNPEP and OXT on endometrium, myometrium, pituitary and corpus luteum (CL). Serum LNPEP concentration were similar. Placental LNPEP U/mg protein was highest in the body and pregnant horn. The highest to lowest LNPEP U/mg protein by tissue were: myometrium > follicle wall > endometrium > kidney > CL > liver. Oxytocin was identified in the equine pituitary, CL and placenta and is likely to act in autocrine or paracrine manner, while LNPEP may act systemically and locally to regulate the availability of OXT.
© 2023. The Author(s).
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Publication Date: 2023-03-21 PubMed ID: 36944665PubMed Central: PMC10030782DOI: 10.1038/s41598-023-31540-9Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper studies the presence and function of oxytocin and a specific enzyme called leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) in pregnant and non-pregnant horses. Scientists set out to investigate how oxytocin, a hormone involved in reproduction and maternal bonding among other actions, and LNPEP are regulated in horse tissues.

Research Methodology

  • The research team conducted the study using serum and tissues from non-pregnant and pregnant horses.
  • In their experiment, they measured serum and tissue levels of LNPEP, an enzyme known to regulate oxytocin. This was done using an ELISA (enzyme-linked immunosorbent assay) from the day of ovulation (D0) until day 21-22.
  • They also looked at samples from horses in periparturition (around the time of birth) and postpartum (after birth).
  • Apart from serum, they evaluated LNPEP in placental tissues and tissues of horses in diestrus (a phase in the reproductive cycle) using protein determinations and ELISA.

Detection and Identification

  • The study also used western blot, a technique used to detect specific proteins in a sample, immunohistochemistry (a method to visually localize proteins in cells of a tissue section) and liquid chromatography-mass spectrometry (LC-MS/MS, a technique used to identify and quantify molecules) to identify the presence of LNPEP and oxytocin in tissues.
  • In addition, they used in situ hybridization, a type of technique to visualize where certain genes are expressed in a tissue, for LNPEP and oxytocin.

Key Findings

  • They found that serum LNPEP concentration were similar in all groups. Meanwhile, placental LNPEP U/mg protein was highest in the body and the pregnant horn (part of the uterus).
  • The study highlighted the highest to lowest LNPEP U/mg protein by tissue were found in: myometrium, follicle wall, endometrium, kidney, corpus luteum, and liver, respectively.
  • Oxytocin was identified in the equine pituitary (a pea-sized gland at the base of the brain), corpus luteum (a temporary endocrine structure in female ovaries), and placenta. The study suggests that oxytocin is likely to act in autocrine or paracrine manner (i.e. it acts on either cells that release it or on nearby cells), while LNPEP may act both systemically and locally to regulate the availability of oxytocin.

Cite This Article

APA
Diel de Amorim M, Dong L, Byron M, Foster RA, Klein C, Saleh M, Saleh T, Card C. (2023). Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare. Sci Rep, 13(1), 4616. https://doi.org/10.1038/s41598-023-31540-9

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 4616
PII: 4616

Researcher Affiliations

Diel de Amorim, Mariana
  • Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA. md649@cornell.edu.
Dong, Lynn
  • Immunopathology Research and Development Laboratory, Department of Biomedical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA.
  • Translational Pathology, Bristol Myers Squibb, Route 206 and Provinceline Rd., Princeton, NJ, 08543, USA.
Byron, Michael
  • Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA.
Foster, Robert A
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, N1G 2W1, Canada.
Klein, Claudia
  • Department of Veterinary Clinical and Diagnostic Science, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
  • Federal Research Institute for Animal Health, Institute of Farm Animal Genetics, Hoeltystr.10, 31535, Neustadt-Mariense, Germany.
Saleh, Monique
  • Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, N1G 2W1, Canada.
Saleh, Tarek
  • Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, N1G 2W1, Canada.
Card, Claire
  • Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. claire.card@usask.ca.

MeSH Terms

  • Horses
  • Animals
  • Female
  • Pregnancy
  • Oxytocin / metabolism
  • Cystinyl Aminopeptidase / metabolism
  • Placenta / metabolism
  • Chromatography, Liquid
  • Tandem Mass Spectrometry

Grant Funding

  • S10 OD017992 / NIH HHS

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 1 times.
  1. Newcombe JR, Cuervo-Arango J, Wilsher S. The Timing of the Maternal Recognition of Pregnancy Is Specific to Individual Mares. Animals (Basel) 2023 May 22;13(10).
    doi: 10.3390/ani13101718pubmed: 37238148google scholar: lookup
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