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Home / Equine Research Bank / J Virol / Characterization of the regulatory functions of the equine h...
Journal of virology1992; 66(2); 936-945; doi: 10.1128/JVI.66.2.936-945.1992

Characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product.

Abstract: Use of the translation-inhibiting drug cycloheximide has indicated that the equine herpesvirus 1 (EHV-1) immediate-early (IE) gene, the sole EHV-1 IE gene, encodes a major viral regulatory protein since IE mRNA translation is a prerequisite for all further viral gene expression (W.L. Gray, R. P. Baumann, A. T. Robertson, G. B. Caughman, D. J. O'Callaghan, and J. Staczek, Virology 158:79-87, 1987). An EHV-1 IE gene expression vector (pSVIE) in combination with chimeric EHV-1 promoter-chloramphenicol acetyltransferase (CAT) reporter constructs was used in transient transfection assays to characterize the regulatory functions of the IE gene product. These experiments demonstrated that (i) the EHV-1 IE gene product is a bifunctional protein capable of both positive and negative modulation of gene expression; (ii) the IE gene product possesses an autoregulatory function which represses the IE promoter; (iii) IE autoregulation is dependent on IE promoter sequences mapping within positions -288 to +73 relative to the transcription initiation site (+1) of the IE gene; (iv) the IE gene product can independently activate the EHV-1 tk promoter (an early promoter) by as much as 60-fold; (v) two EHV-1 beta-gamma (leaky late) promoters, those of IR5 (gene 5 in the inverted repeat) and the glycoprotein D gene, demonstrate a requirement for both the IE gene product as well as a gene product encoded within the EHV-1 XbaI G fragment for significant activation; and (vi) the IE gene product is capable of activating heterologous viral promoters.
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Publication Date: 1992-02-01 PubMed ID: 1309921PubMed Central: PMC240795DOI: 10.1128/JVI.66.2.936-945.1992Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research revolves around the regulatory roles of the equine herpesvirus 1 (EHV-1) immediate-early (IE) gene product, where findings indicate that it acts as a key modulator of the virus’ gene expression.

An Overview of the Research Objectives and Methodology

  • The researchers aimed to examine and elucidate the regulatory functions of the EHV-1 IE gene product. The principal tool used in the study was the translation-inhibiting drug cycloheximide which gave an indication about the IE gene’s role as a major regulatory protein for the virus.
  • This was followed by the construction of an EHV-1 IE gene expression vector known as pSVIE, in combination with chimeric EHV-1 promoter-chloramphenicol acetyltransferase (CAT) reporter constructs. This was used in executing transient transfection assays for better characterization of the IE gene product’s regulatory functions.

Key Findings of the Research

  • The researchers found that the EHV-1 IE gene product is a bifunctional protein, capable of both enhancing and suppressing gene expression. This dual role underscores its importance in the virus’ lifecycle.
  • The IE gene product was found to possess an autoregulatory function that suppresses the IE promoter. The presence of such negative feedback mechanisms are common in biological systems for maintaining balance and ensuring controlled reactions.
  • The study also identified specific positions on the IE promoter, namely from -288 to +73 relative to the transcription initiation site, that are critical to IE autoregulation.
  • Another key finding was that the IE gene product could independently boost the activity of the EHV-1 tk promoter, an early promoter, by up to 60-fold. This highlights the potential of the IE gene product in fast-tracking the launch of the viral gene expression.
  • The study also indicated that two EHV-1 beta-gamma (leaky late) promoters, those of IR5 (gene 5 in the inverted repeat) and the glycoprotein D gene, needed both the IE gene product and a gene product encoded within the EHV-1 XbaI G fragment for significant activation.
  • Furthermore, the researchers discovered that the IE gene product could activate heterologous viral promoters, i.e., promoters that are part of different viruses. This suggests that the IE gene product might play a crucial role in viral cross-talk or interaction.

Cite This Article

APA
Smith RH, Caughman GB, O'Callaghan DJ. (1992). Characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product. J Virol, 66(2), 936-945. https://doi.org/10.1128/JVI.66.2.936-945.1992

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 66
Issue: 2
Pages: 936-945

Researcher Affiliations

Smith, R H
  • Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
Caughman, G B
    O'Callaghan, D J

      MeSH Terms

      • Animals
      • Chloramphenicol O-Acetyltransferase / genetics
      • Chloramphenicol O-Acetyltransferase / metabolism
      • Cloning, Molecular
      • Gene Expression Regulation, Viral
      • Genes, Viral
      • Genetic Vectors
      • Herpesvirus 1, Equid / genetics
      • L Cells
      • Mice
      • Plasmids
      • Promoter Regions, Genetic
      • Restriction Mapping
      • Trans-Activators / genetics
      • Transfection
      • Viral Proteins / genetics

      Grant Funding

      • AI 22001 / NIAID NIH HHS

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