FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Metabolomics / Characterization of trotter horses urine metabolome by means...
Metabolomics : Official journal of the Metabolomic Society2018; 14(8); 106; doi: 10.1007/s11306-018-1403-3

Characterization of trotter horses urine metabolome by means of proton nuclear magnetic resonance spectroscopy.

Abstract: Metabolomics has been recognized as a powerful approach for disease screening. In order to highlight potential health issues in subjects, a key factor is the possibility to compare quantitatively the metabolome of their biofluids with reference values from healthy individuals. Such efforts towards the systematic characterization of the metabolome of biofluids in perfect health conditions, far from concluded for humans, have barely begun on horses. The present work attempts, for the first time, to give reference quantitative values for the molecules mostly represented in the urine metabolome of horses at rest and under light training, as observable by 1H-NMR. The metabolome of ten trotter horses, four male and six female, ranging from 3 to 8 years of age, has been observed by 1H-NMR spectroscopy before and after three training sessions. We could characterize and quantify 54 molecules in trotter horse urine, originated from diet, protein digestion, energy generation or gut-microbial co-metabolism. We were able to describe how gender, age and exercise affected their concentration, by means of a two steps protocol based on univariate and robust principal component analysis.
Get Full Text
Publication Date: 2018-08-03 PubMed ID: 30830366DOI: 10.1007/s11306-018-1403-3Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to establish reference values for common molecules in healthy trotter horses’ urine metabolomes, using proton nuclear magnetic resonance spectroscopy. It also analyzed the impact of gender, age, and exercise on these molecular concentrations.

Study Overview

  • The study used metabolomics, a scientific method that looks at the unique chemical fingerprints that specific cellular processes leave behind. This approach is particularly useful for disease screening and identifying potential health issues.
  • The researchers aimed to establish a baseline or reference values for the urine metabolome in healthy horses, a task that is yet to be fully accomplished for even humans.
  • Using proton nuclear magnetic resonance (H-NMR) spectroscopy, the research team analyzed the urine of ten trotter horses (four males and six females, aged between 3 and 8 years) before and after three training sessions.

Findings and Analysis

  • The researchers identified a total of 54 molecules in the urine of trotter horses, which they traced back to a variety of sources such as diet, protein digestion, energy generation, or gut-microbial co-metabolism.
  • Part of the research focused on understanding how factors such as gender, age, and physical exercise affect the concentration levels of these molecules in the horse’s urine. A two-step protocol involving univariate and robust principal component analysis was used for the purpose.

Significance of the Research

  • This research marks the first attempt to quantify the common molecules in the urine metabolome of trotter horses, which will subsequently serve as useful reference points for gauging their health condition.
  • The discoveries made can also amplify our understanding of how various factors can influence metabolomic composition.
  • Such investigations can potentially lead to better disease screening processes and contribute to overall animal health management strategies.

Cite This Article

APA
Zhu C, Faillace V, Laus F, Bazzano M, Laghi L. (2018). Characterization of trotter horses urine metabolome by means of proton nuclear magnetic resonance spectroscopy. Metabolomics, 14(8), 106. https://doi.org/10.1007/s11306-018-1403-3

Publication

Metabolomics : Official journal of the Metabolomic Society
ISSN: 1573-3890
NlmUniqueID: 101274889
Country: United States
Language: English
Volume: 14
Issue: 8
Pages: 106

Researcher Affiliations

Zhu, Chenglin
  • Department of Agro-Food Science and Technology, Centre of Foodomics, University of Bologna, Cesena, Italy.
Faillace, Vanessa
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy.
Laus, Fulvio
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy.
Bazzano, Marilena
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy.
Laghi, Luca
  • Department of Agro-Food Science and Technology, Centre of Foodomics, University of Bologna, Cesena, Italy. l.laghi@unibo.it.

MeSH Terms

  • Animals
  • Female
  • Horses
  • Male
  • Metabolome
  • Proton Magnetic Resonance Spectroscopy / methods
  • Urinalysis / methods

References

This article includes 30 references
  1. J Am Vet Med Assoc. 2012 Jan 15;240(2):205-12
    pubmed: 22217030
  2. Equine Vet J. 1988 Jan;20(1):41-5
    pubmed: 3366105
  3. Mol Biosyst. 2011 Sep;7(9):2577-88
    pubmed: 21761043
  4. J Agric Food Chem. 2015 May 27;63(20):4973-8
    pubmed: 25966609
  5. Equine Vet J. 2015 Nov;47(6):721-30
    pubmed: 25130591
  6. J Appl Physiol (1985). 2018 Aug 1;125(2):654-660
    pubmed: 29672225
  7. Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1420-4
    pubmed: 18230739
  8. Clin Chim Acta. 1959 Mar;4(2):265-71
    pubmed: 13639272
  9. Respir Res. 2005 Jul 14;6:71
    pubmed: 16018807
  10. Am J Clin Nutr. 2016 Nov;104(5):1253-1262
    pubmed: 27733402
  11. PLoS One. 2014 Feb 04;9(2):e87424
    pubmed: 24504261
  12. NMR Biomed. 2005 May;18(3):143-62
    pubmed: 15627238
  13. Anal Chem. 2006 Jul 1;78(13):4281-90
    pubmed: 16808434
  14. Biochimie. 2015 Dec;119:146-65
    pubmed: 26542286
  15. J Transl Med. 2015 Jul 25;13:243
    pubmed: 26208967
  16. Appl Spectrosc. 2010 Sep;64(9):1007-16
    pubmed: 20828437
  17. Anal Biochem. 2006 May 15;352(2):274-81
    pubmed: 16600169
  18. Med Sci Sports Exerc. 2010 Jun;42(6):1162-73
    pubmed: 20479615
  19. Anal Chem. 2007 Sep 15;79(18):6995-7004
    pubmed: 17702530
  20. Clin Pharmacol Ther. 1984 Jul;36(1):133-7
    pubmed: 6734043
  21. Ther Apher Dial. 2011 Apr;15(2):125-8
    pubmed: 21426501
  22. J Chromatogr A. 2017 Oct 27;1521:90-99
    pubmed: 28941809
  23. J Appl Physiol. 1972 Mar;32(3):304-8
    pubmed: 5010039
  24. J Proteome Res. 2017 May 5;16(5):1847-1856
    pubmed: 28345344
  25. Nucleic Acids Res. 2007 Jan;35(Database issue):D521-6
    pubmed: 17202168
  26. NMR Biomed. 2008 Mar;21(3):195-207
    pubmed: 17474139
  27. Free Radic Res. 2000 Mar;32(3):235-44
    pubmed: 10730822
  28. PLoS One. 2018 Mar 22;13(3):e0194827
    pubmed: 29566085
  29. Xenobiotica. 1999 Nov;29(11):1181-9
    pubmed: 10598751
  30. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1312-8
    pubmed: 5244739

Citations

This article has been cited 13 times.
  1. Corradini I, Jose-Cunilleras E, Nolis P, López-Murcia MM, Mayordomo-Febrer A. Exploratory Metabolomic Fingerprinting of Aqueous Humor in Healthy Horses and Donkeys, and in Horses with Ocular Pathologies. Animals (Basel) 2025 Sep 26;15(19).
    doi: 10.3390/ani15192810pubmed: 41096405google scholar: lookup
  2. Laus F, Bazzano M, Spaterna A, Laghi L, Marchegiani A. Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment. Metabolites 2024 May 7;14(5).
    doi: 10.3390/metabo14050269pubmed: 38786746google scholar: lookup
  3. Zhu C, Zhang Q, Zhao X, Yang Z, Yang F, Yang Y, Tang J, Laghi L. Metabolomic Analysis of Multiple Biological Specimens (Feces, Serum, and Urine) by (1)H-NMR Spectroscopy from Dairy Cows with Clinical Mastitis. Animals (Basel) 2023 Feb 19;13(4).
    doi: 10.3390/ani13040741pubmed: 36830529google scholar: lookup
  4. Lisuzzo A, Laghi L, Fiore F, Harvatine K, Mazzotta E, Faillace V, Spissu N, Zhu C, Moscati L, Fiore E. Evaluation of the metabolomic profile through (1)H-NMR spectroscopy in ewes affected by postpartum hyperketonemia. Sci Rep 2022 Oct 1;12(1):16463.
    doi: 10.1038/s41598-022-20371-9pubmed: 36183000google scholar: lookup
  5. Zhu C, Jin L, Luo B, Zhou Q, Dong L, Li X, Zhang H, Huang Y, Li C, Zou L, Laghi L. Dominant Components of the Giant Panda Seminal Plasma Metabolome, Characterized by (1)H-NMR Spectroscopy. Animals (Basel) 2022 Jun 14;12(12).
    doi: 10.3390/ani12121536pubmed: 35739871google scholar: lookup
  6. Lisuzzo A, Laghi L, Faillace V, Zhu C, Contiero B, Morgante M, Mazzotta E, Gianesella M, Fiore E. Differences in the serum metabolome profile of dairy cows according to the BHB concentration revealed by proton nuclear magnetic resonance spectroscopy ((1)H-NMR). Sci Rep 2022 Feb 15;12(1):2525.
    doi: 10.1038/s41598-022-06507-xpubmed: 35169190google scholar: lookup
  7. Zhu C, Tang K, Lu X, Tang J, Laghi L. An Untargeted Metabolomics Investigation of Milk from Dairy Cows with Clinical Mastitis by (1)H-NMR. Foods 2021 Jul 23;10(8).
    doi: 10.3390/foods10081707pubmed: 34441485google scholar: lookup
  8. Bazzano M, Laghi L, Zhu C, Lotito E, Sgariglia S, Tesei B, Laus F. Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by (1)H-NMR. Metabolites 2020 Jul 21;10(7).
    doi: 10.3390/metabo10070298pubmed: 32708237google scholar: lookup
  9. Bazzano M, Laghi L, Zhu C, Magi GE, Tesei B, Laus F. Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses. BMC Vet Res 2020 Jul 8;16(1):233.
    doi: 10.1186/s12917-020-02446-9pubmed: 32641035google scholar: lookup
  10. Zhu C, Fasoli S, Isani G, Laghi L. First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy. Metabolites 2020 Apr 17;10(4).
    doi: 10.3390/metabo10040157pubmed: 32316507google scholar: lookup
  11. Zhu C, Petracci M, Li C, Fiore E, Laghi L. An Untargeted Metabolomics Investigation of Jiulong Yak (Bos grunniens) Meat by (1)H-NMR. Foods 2020 Apr 12;9(4).
    doi: 10.3390/foods9040481pubmed: 32290528google scholar: lookup
  12. Venosi S, Ceccarelli G, de Angelis M, Laghi L, Bianchi L, Martinelli O, Maruca D, Cavallari EN, Toscanella F, Vassalini P, Trinchieri V, Oliva A, d'Ettorre G. Infected chronic ischemic wound topically treated with a multi-strain probiotic formulation: a novel tailored treatment strategy. J Transl Med 2019 Nov 9;17(1):364.
    doi: 10.1186/s12967-019-2111-0pubmed: 31706326google scholar: lookup
  13. Zhu C, Li C, Wang Y, Laghi L. Characterization of Yak Common Biofluids Metabolome by Means of Proton Nuclear Magnetic Resonance Spectroscopy. Metabolites 2019 Mar 2;9(3).
    doi: 10.3390/metabo9030041pubmed: 30832316google scholar: lookup
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.