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Veterinary world2022; 15(11); 2673-2680; doi: 10.14202/vetworld.2022.2673-2680

Characterizing the antimicrobial resistance profile of Escherichia coli found in sport animals (fighting cocks, fighting bulls, and sport horses) and soils from their environment.

Abstract: Antimicrobial resistance (AMR) is a significant threat to global health and development. Inappropriate antimicrobial drug use in animals cause AMR, and most studies focus on livestock because of the widespread use of antimicrobial medicines. There is a lack of studies on sports animals and AMR issues. This study aimed to characterize the AMR profile of E. coli found in sports animals (fighting cocks, fighting bulls, and sport horses) and soils from their environment. Unassigned: Bacterial isolation and identification were conducted to identify E. coli isolates recovered from fresh feces that were obtained from fighting cocks (n = 32), fighting bulls (n = 57), sport horses (n = 33), and soils from those farms (n = 32) at Nakhon Si Thammarat. Antimicrobial resistance was determined using 15 tested antimicrobial agents - ampicillin (AM), amoxicillin-clavulanic acid, cephalexin (CN), cefalotin (CF), cefoperazone, ceftiofur, cefquinome, gentamicin, neomycin, flumequine (UB), enrofloxacin, marbofloaxacin, polymyxin B, tetracycline (TE), and sulfamethoxazole/trimethoprim (SXT). The virulence genes, AMR genes, and phylogenetic groups were also examined. Five virulence genes, iroN, ompT, hlyF, iss, and iutA, are genes determining the phylogenetic groups, chuA, cjaA, and tspE4C2, were identified. The AMR genes selected for detection were blaTEM and blaSHV for the beta-lactamase group; cml-A for phenicol; dhfrV for trimethoprim; sul1 and sul2 for sulfonamides; tetA, tetB, and tetC for TEs; and qnrA, qnrB, and qnrS for quinolones. Unassigned: The E. coli derived from sports animals were resistant at different levels to AM, CF, CN, UB, SXT, and TE. The AMR rate was overall higher in fighting cocks than in other animals, with significantly higher resistance to AM, CF, and TE. The highest AMR was found in fighting cocks, where 62.5% of their isolates were AM resistant. In addition, multidrug resistance was highest in fighting cocks (12.5%). One extended-spectrum beta-lactamase E. coli isolate was found in the soils, but none from animal feces. The phylogenetic analysis showed that most E. coli isolates were in Group B1. The E. coli isolates from fighting cocks had more virulence and AMR genes than other sources. The AMR genes found in 20% or more of the isolates were blaTEM (71.9%), qnrB (25%), qnrS (46.9%), and tetA (56.25%), whereas in the E. coli isolates collected from soils, the only resistance genes found in 20% or more of the isolates were blaTEM (30.8%), and tetA (23.1%). Unassigned: Escherichia coli from fighting cock feces had significantly higher resistance to AM, CF, and TE than isolates from other sporting animals. Hence, fighting cocks may be a reservoir of resistant E. coli that can transfer to the environment and other animals and humans in direct contact with the birds or the birds' habitat. Programs for antimicrobial monitoring should also target sports animals and their environment.
Copyright: © Wongtawan, et al.
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Publication Date: 2022-11-25 PubMed ID: 36590125PubMed Central: PMC9798048DOI: 10.14202/vetworld.2022.2673-2680Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores antimicrobial resistance (AMR) profiles in sports animals including fighting cocks, fighting bulls, and sports horses, as well as the soil in their environments. In particular, the study investigates the AMR in the Escherichia coli bacteria from these sources, drawing attention to the need for monitoring programs for sports animals and their habitats.

Methodology

  • Researchers gathered E. coli from fresh feces of fighting cocks, fighting bulls, sports horses, and soils from their farms in Nakhon Si Thammarat.
  • The antimicrobial resistance of E. coli isolates was tested against 15 antimicrobial agents such as ampicillin, cephalothin, ceftiofur, and sulfamethoxazole/trimethoprim.
  • The study also scanned for the presence of various virulence genes, AMR genes, and identified the phylogenetic groups.

Findings

  • The E. coli isolates derived from sports animals showed varying degrees of resistance to different antimicrobial agents.
  • Particularly, the resistance level was significantly higher in fighting cocks, especially to ampicillin, cefalotin, and tetracycline. Moreover, multi-drug resistance was primarily observed in fighting cocks.
  • Interestingly, the study found an extended-spectrum beta-lactamase (ESBL) E. coli isolate in the soil samples, possibly hinting at environment-AMR linkage.
  • On analyzing phylogenetic groups, most E. coli isolates fit into Group B1.
  • E. coli from fighting cocks contained more virulence and AMR genes than other sources.

Significance and Recommendations

  • The study points out the notably higher resistance of E. coli found in fighting cock feces, suggesting that these birds could be carriers of resistant E. coli which may spread to the environment, other animals, and possibly humans in contact.
  • This highlights that there is a potential risk of AMR spread from sports animals, which were not traditionally included in antimicrobial monitoring programs. Therefore, the authors recommended the inclusion of sports animals and their environment into AMR monitoring programs.

Cite This Article

APA
Wongtawan T, Narinthorn R, Sontigun N, Sansamur C, Petcharat Y, Fungwithaya P, Saengsawang P, Blackall PJ, Thomrongsuwannakij T. (2022). Characterizing the antimicrobial resistance profile of Escherichia coli found in sport animals (fighting cocks, fighting bulls, and sport horses) and soils from their environment. Vet World, 15(11), 2673-2680. https://doi.org/10.14202/vetworld.2022.2673-2680

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 15
Issue: 11
Pages: 2673-2680

Researcher Affiliations

Wongtawan, Tuempong
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Excellence Centre for Melioidosis and other microorganisms, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Narinthorn, Ruethai
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Sontigun, Narin
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Excellence Centre for Melioidosis and other microorganisms, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Sansamur, Chalutwan
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Petcharat, Yotsapat
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Fungwithaya, Punpichaya
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Excellence Centre for Melioidosis and other microorganisms, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Saengsawang, Phirabhat
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
Blackall, Patrick J
  • Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia 4067, Australia.
Thomrongsuwannakij, Thotsapol
  • Akkhraratchakumari Veterinary College, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.
  • Centre for One Health, Walailak University, Thai Buri, Tha Sala, Nakhon Si Thammarat 80160, Thailand.

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 55 references
  1. World Health Organization. Antimicrobial Resistance:Global Report on Surveillance. Geneva: World Health Organization; 2014. pp. 1–256.
  2. O'Neill J. Review on Antimicrobial Resistance:Tackling Drug-resistant Infections Globally:Final Report and Recommendations. Welcome Trust, London. 2016:1–84.
  3. Holmes A.H, Moore L.S, Sundsfjord A, Steinbakk M, Regmi S, Karkey A, Piddock L.J.V, Guerin P.J. Understanding the mechanisms and drivers of antimicrobial resistance. Lancet 2016;387(10014):176–187.
    pubmed: 26603922
  4. National Academies of Sciences, Engineering, and Medicine. The cost dimensions of antimicrobial resistance. In:Understanding the Economics of Microbial Threats:Proceedings of a Workshop:The National Academies Press, United States. 2018.
    pubmed: 30525340
  5. World Health Organization. Antibiotic Resistance:Multi-country Public Awareness Survey. Geneva: World Health Organization; 2015. [Retrieved on 01-03-2022]. Available from: https://www.apps.who.int/iris/handle/10665/194460.
  6. Álvarez-Narváez S, Berghaus L.J, Morris E, Willingham-Lane J.M, Slovis N.M, Giguere S. A common practice of widespread antimicrobial use in horse production promotes multi-drug resistance. Sci. Rep. 2020;10(1):1–13.
    pmc: PMC6976650pubmed: 31969575
  7. Van Cleven A, Sarrazin S, De Rooster H, Paepe D, Van der Meeren S, Dewulf J. Antimicrobial prescribing behaviour in dogs and cats by Belgian veterinarians. Vet. Rec. 2018;182(11):324–324.
    pubmed: 29196488
  8. Hardefeldt L.Y, Selinger J, Stevenson M.A, Gilkerson J.R, Crabb H, Billman-Jacobe H, Thursky K, Bailey K.E, Awad M, Browning G.F. Population wide assessment of antimicrobial use in dogs and cats using a novel data source-a cohort study using pet insurance data. Vet. Microbiol. 2018;225:34–39.
    pubmed: 30322530
  9. Kaewnoi D, Wiriyaprom R, Indoung S. Gastrointestinal parasite infections in fighting bulls in South Thailand. Vet. World 2020;13(8):1544–1548.
    pmc: PMC7522952pubmed: 33061225
  10. Keck N, Boschiroli M.L, Smyej F, Vogler V, Desvaux S, Moyen J.L. Successful application of the gamma-interferon assay in a bovine tuberculosis eradication program:the French bullfighting herd experience. Front. Vet. Sci. 2018;5:27.
    pmc: PMC5835129pubmed: 29536019
  11. Murata M, Takahashi H, Takahashi S, Takahashi Y, Chibana H, Murata Y, Sugiyama K, Kaneshima T, Yamaguchi S, Miyasato H, Murakami M, Kano R, Hasegawa A, Uezato H, Hosokawa A. Isolation of Microsporum gallinae from a fighting cock (Gallus gallus domesticus) in Japan. Med. Mycol. 2013;51(2):144–149.
    pubmed: 22809243
  12. Paul M. C, Figuié M, Kovitvadhi A, Valeix S, Wongnarkpet S, Poolkhet C, Binot A. Collective resistance to HPAI H5N1 surveillance in the Thai cockfighting community:Insights from a social anthropology study. Prev. Vet. Med. 2015;120(1):106–114.
    pubmed: 25800453
  13. Steinman A, Navon-Venezia S. Antimicrobial resistance in horses. Animals 2020;10(7):1161.
    pmc: PMC7401552pubmed: 32659916
  14. Chaiyakot P, Visuthismajarn P. A pattern of rural tourism in the Songkhla Lake Basin, Thailand. Int. J. Manag. Inf. Syst. 2012;16(2):173–176.
  15. Hata A, Nunome M, Suwanasopee T, Duengkae P, Chaiwatana S, Chamchumroon W, Suzuki T, Koonawootrittriron S, Matsuda Y, Srikulnath K. Origin and evolutionary history of domestic chickens inferred from a large population study of Thai red junglefowl and indigenous chickens. Sci. Rep. 2021;11(1):2035.
    pmc: PMC7820500pubmed: 33479400
  16. Perry-Gal L, Erlich A, Gilboa A, Bar-Oz G. Earliest economic exploitation of chicken outside East Asia:Evidence from the Hellenistic Southern Levant. PNAS 2015;112(32):9849–9854.
    pmc: PMC4538678pubmed: 26195775
  17. Cohen E. Bullfighting and tourism. Tour Anal. 2014;19(5):545–556.
  18. Choprakarn K, Wongpichet K. Village Chicken Production Systems in Thailand. In:Proceedings of the International Poultry Conference, Bangkok, Thailand. 2007:63–64.
  19. Hesp A, Braak C.T, van der Goot J, Veldman K, van Schaik G, Mevius D. Antimicrobial resistance clusters in commensal Escherichia coli from livestock. Zoonoses Public Health 2021;68(3):194–202.
    pmc: PMC8048968pubmed: 33455079
  20. Nhung N.T, Cuong N.V, Thwaites G, Carrique-Mas J. Antimicrobial usage and antimicrobial resistance in animal production in Southeast Asia:A review. Antibiotics (Basel) 2016;5(4):37.
    pmc: PMC5187518pubmed: 27827853
  21. Gwenzi W, Chaukura N, Muisa-Zikali N, Teta C, Musvuugwa T, Rzymski P, Abia A.L.K. Insects, rodents, and pets as reservoirs, vectors, and sentinels of antimicrobial resistance. Antibiotics (Basel) 2021;10(1):68.
    pmc: PMC7826649pubmed: 33445633
  22. Leite-Martins L.R, Mahú M.I, Costa A.L, Mendes Â, Lopes E, Mendonça D.M, Niza-Ribeiro J.J, de Matos A.J, da Costa P.M. Prevalence of antimicrobial resistance in enteric Escherichia coli from domestic pets and assessment of associated risk markers using a generalized linear mixed model. Prev. Vet. Med. 2014;117(1):28–39.
    pubmed: 25294317
  23. Maddox T.W, Clegg P.D, Williams N.J, Pinchbeck G.L. Antimicrobial resistance in bacteria from horses:Epidemiology of antimicrobial resistance. Equine Vet. J. 2015;47(6):756–765.
    pubmed: 26084443
  24. Sadikalay S, Reynaud Y, Guyomard-Rabenirina S, Falord M, Ducat C, Fabre L, Le Hello S, Talarmin A, Ferdinand S. High genetic diversity of extended-spectrum β-lactamases producing Escherichia coli in feces of horses. Vet. Microbiol. 2018;219:117–122.
    pubmed: 29778183
  25. Chung Y.S, Song J.W, Kim D.H, Shin S, Park Y.K, Yang S.J, Lim S.K, Park K.T, Park Y.H. Isolation and characterization of antimicrobial-resistant Escherichia coli from national horse racetracks and private horse-riding courses in Korea. J. Vet. Sci. 2016;17(2):199–206.
    pmc: PMC4921668pubmed: 26645344
  26. Sato W, Sukmawinata E, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, Sueyoshi M. Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan. J. Equine Sci. 2020;31(4):85–91.
    pmc: PMC7750643pubmed: 33376444
  27. Kawamura K, Nagano N, Suzuki M, Wachino J.I, Kimura K, Arakawa Y. ESBL-producing Escherichia coli and its rapid rise among healthy people. Food Saf. 2017;5(4):122–150.
    pmc: PMC6989193pubmed: 32231938
  28. Malande O. O, Nuttall J, Pillay V, Bamford C, Eley B. A ten-year review of ESBL and non-ESBL Escherichia coli bloodstream infections among children at a tertiary referral hospital in South Africa. PLoS One 2019;14(9):e0222675.
    pmc: PMC6759190pubmed: 31550295
  29. McDonald K.L, Garland S, Carson C.A, Gibbens K, Parmley E.J, Finley R, MacKinnon M. C. Measures used to assess the burden of ESBL-producing Escherichia coli infections in humans:A scoping review. JAC Antimicrob. Resist. 2021;3(1):dlaa104.
    pmc: PMC8210151pubmed: 34223063
  30. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals;Approved Standard. 4th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2013. pp. 1–94.
  31. Johnson T.J, Wannemuehler Y, Doetkott C, Johnson S.J, Rosenberger S.C, Nolan L.K. Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. J. Clin. Microbiol. 2008;46(12):3987–3996.
    pmc: PMC2593276pubmed: 18842938
  32. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl. Environ. Microbiol. 2000;66(10):4555–4558.
    pmc: PMC92342pubmed: 11010916
  33. Van T.T.H, Chin J, Chapman T, Tran L.T, Coloe P.J. Safety of raw meat and shellfish in Vietnam:An analysis of Escherichia coli isolations for antibiotic resistance and virulence genes. Int. J. Food Microbiol. 2008;124(3):217–223.
    pubmed: 18457892
  34. Maynard C, Fairbrother J.M, Bekal S, Sanschagrin F, Levesque R.C, Brousseau R, Masson L, Lariviere S, Harel J. Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149:K91 isolates obtained over a 23-year period from pigs. Antimicrob. Agents Chemother. 2003;47(10):3214–3221.
    pmc: PMC201132pubmed: 14506033
  35. Sandvang D, Aarestrup F.M, Jensen L.B. Characterisation of integrons and antibiotic resistance genes in Danish multiresistant Salmonella enterica Typhimurium DT104. FEMS Microbiol. Lett. 1998;160(1):37–41.
    pubmed: 9495010
  36. Sengeløv G, Agersø Y, Halling-Sørensen B, Baloda S.B, Andersen J.S, Jensen L.B. Bacterial antibiotic resistance levels in Danish farmland as a result of treatment with pig manure slurry. Environ. Int. 2003;28(7):587–595.
    pubmed: 12504155
  37. Gay K, Robicsek A, Strahilevitz J, Park C.H, Jacoby G, Barrett T.J, Medalla F, Chiller T.M, Hooper D.C. Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica. Clin. Infect. Dis. 2006;43(3):297–304.
    pubmed: 16804843
  38. Wang M, Sahm D.F, Jacoby G.A, Hooper D.C. Emerging plasmid-mediated quinolone resistance associated with the qnr gene in Klebsiella pneumoniae clinical isolates in the United States. Antimicrob. Agents Chemother. 2004;48(4):1295–1299.
    pmc: PMC375335pubmed: 15047532
  39. Thomrongsuwannakij T, Blackall P.J, Chansiripornchai N. A study on Campylobacter jejuni and Campylobacter coli through commercial broiler production chains in Thailand:antimicrobial resistance, the characterization of DNA gyrase subunit A mutation, and genetic diversity by flagellin A gene restriction fragment length polymorphism. Avian Dis. 2017;61(2):186–197.
    pubmed: 28665716
  40. Team R.C. A Language and Environment for Statistical Computing. R Foundation for Statistical Computing Vienna, Austria. 2020.
  41. Massella E, Giacometti F, Bonilauri P, Reid C.J, Djordjevic S.P, Merialdi G, Bacci C, Fiorentini L, Massi P, Bardasi L, Rubini S. Antimicrobial resistance profile and ExPEC virulence potential in commensal Escherichia coli of multiple sources. Antibiotics 2021;10(4):351.
    pmc: PMC8067153pubmed: 33810387
  42. Kolenda R, Burdukiewicz M, Schierack P. A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front. Cell. Infect. Microbiol. 2015;5:23.
    pmc: PMC4357325pubmed: 25815276
  43. Johnson T.J, Logue C.M, Johnson J.R, Kuskowski M.A, Sherwood J.S, Barnes H.J, DebRoy C, Wannemuehler Y.M, Obata-Yasuoka M, Spanjaard L, Nolan L.K. Associations between multidrug resistance, plasmid content, and virulence potential among extraintestinal pathogenic and commensal Escherichia coli from humans and poultry. Foodborne Pathog. Dis. 2012;9(1):37–46.
    pmc: PMC3250628pubmed: 21988401
  44. Hinthong W, Pumipuntu N, Santajit S, Kulpeanprasit S, Buranasinsup S, Sookrung N, Chaicumpa W, Aiumurai P, Indrawattana N. Detection and drug resistance profile of Escherichia coli from subclinical mastitis cows and water supply in dairy farms in Saraburi Province, Thailand. PeerJ 2017;5:e3431.
    pmc: PMC5472034pubmed: 28626609
  45. Nittayasut N, Yindee J, Boonkham P, Yata T, Suanpairintr N, Chanchaithong P. Multiple and high-risk clones of extended-spectrum cephalosporin-resistant and blaNDM-5-harbouring uropathogenic Escherichia coli from cats and dogs in Thailand. Antibiotics (Basel) 2021;10(11):1374.
    pmc: PMC8614778pubmed: 34827312
  46. Thomrongsuwannakij T, Narinthorn R, Mahawan T, Blackall P.J. Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens. Poult. Sci. 2022;101(1):101527.
    pmc: PMC8627976pubmed: 34823179
  47. Thongratsakul S, Poolkhet C, Amavisit P, Sato T, Fukuda A, Usui M, Tamura Y. Antimicrobial resistance and STEC virulence genes of Escherichia coli isolated from non-diarrheic and diarrheic dogs at a veterinary teaching hospital in Thailand. Southeast Asian J. Trop. Med. Public Hlth. 2019;50(4):708–714.
  48. Yiğin A. Antimicrobial resistance and extended-spectrum beta-lactamase (ESBL) genes in E. coli isolated from equine fecal samples in Turkey. J. Equine Vet. Sci. 2021;101:103461.
    pubmed: 33993943
  49. Elias L, Gillis D.C, Gurrola-Rodriguez T, Jeon J.H, Lee J.H, Kim T.Y, Lee S.H, Murray S.A, Ohta N, Scott H.M, Wu J. The occurrence and characterization of extended-spectrum-beta-lactamase-producing Escherichia coli isolated from clinical diagnostic specimens of equine origin. Animals 2019;10(1):28.
    pmc: PMC7022413pubmed: 31877788
  50. Schnepf A, Bienert-Zeit A, Ertugrul H, Wagels R, Werner N, Hartmann M, Feige K, Kreienbrock L. Antimicrobial usage in horses:the use of electronic data, data curation, and first results. Front. Vet. Sci. 2020;7:216.
    pmc: PMC7200993pubmed: 32411737
  51. Salah F.D, Soubeiga S.T, Ouattara A.K, Sadji A.Y, Metuor-Dabire A, Obiri-Yeboah D, Banla-Kere A, Karou S, Simpore J. Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lome, Togo. Antimicrob. Resist. Infect. Control. 2019;8:104.
    pmc: PMC6582466pubmed: 31244995
  52. Younis G, Awad A, Mohamed N. Phenotypic and genotypic characterization of antimicrobial susceptibility of avian pathogenic Escherichia coli isolated from broiler chickens. Vet. World 2017;10(10):1167.
    pmc: PMC5682260pubmed: 29184361
  53. Seo K.W, Lee Y.J. Detection of plasmid-mediated quinolone resistance genes in β-lactamase-producing Escherichia coli isolates from layer hens. Poult. Sci. 2019;98(3):1480–1487.
    pubmed: 30496543
  54. Razavi M, Marathe N.P, Gillings M.R, Flach C.F, Kristiansson E, Joakim Larsson D.G. Discovery of the fourth mobile sulfonamide resistance gene. Microbiome 2017;5(1):1–12.
    pmc: PMC5732528pubmed: 29246178
  55. Kim Y.B, Yoon M.Y, Ha J. S, Seo K. W, Noh E. B, Son S. H, Lee Y. J. Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis. Poult. Sci. 2020;99(2):1088–1095.
    pmc: PMC7587703pubmed: 32029145
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