Characterizing the meiotic spindle configuration and chromosome complement of in vivo matured equine oocytes.
Abstract: No abstract available
Publication Date: 2010-05-20 PubMed ID: 33437111PubMed Central: PMC7799372DOI: 10.1016/j.anireprosci.2010.04.136Google Scholar: Lookup
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Summary
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This study aims to explore the reasons for early embryonic loss in horses, especially in older mares, by examining the organization of the meiotic spindle and interactions between chromosomes in equine oocytes (egg cells).
Introduction
- The research paper focuses on the significant problem of early embryonic loss in horses, which causes substantial economic loss and affects reproductive efficiency.
- Typically, the chances of embryonic loss between 12 and 60 days of gestation increase from 10% to 20-30% for mares aged over 18 years.
- The study suggests that oocytes from horses above 18 years old have a high number of inherent defects leading to early embryonic loss, drawing parallels with decreased oocyte quality in older women due to abnormal chromosome-microtubule interactions at the meiotic spindle.
- The researchers hypothesize that similar aging-related alterations in meiotic spindle organization and chromosome interactions could contribute to a high incidence of early embryonic loss in older mares.
Objective
- This study aims to assess the meiotic spindle organization and chromosome complement in equine oocytes by using three fluorescent antibody probes.
- The outcomes of this study will be further used for comparing these characteristics in young and older mares.
Materials and Methods
- The research involves a sample of eight mares, aged between 4 to 10 years, with an average weight between 300-500 kg.
- The reproductive tract of each horse was examined using transrectal palpation and ultrasonography.
- When an ovarian follicle with at least a 35mm diameter developed in conjunction with significant endometrial edema, each mare was injected with 2500 IU human chorionic gonadotropin (a hormone) intravenously.
- The mares were then used for oocyte collection via a process called transvaginal ultrasound-guided follicle aspiration (a method where a needle is guided into an ovary to collect eggs) 28-32 hours later.
- The oocytes were then transferred for laboratory identification and evaluation.
Cite This Article
APA
Vanderwall DK, Baumann C, Viveiros M, Sertich PL, Kelleman AA, Maenhoudt C, Jacobson CC, De La Fuente R.
(2010).
Characterizing the meiotic spindle configuration and chromosome complement of in vivo matured equine oocytes.
Anim Reprod Sci, 121(1-2 Suppl), 234-236.
https://doi.org/10.1016/j.anireprosci.2010.04.136 Publication
Researcher Affiliations
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Animal Biology, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania,382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
- Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.
Grant Funding
- R01 HD042740 / NICHD NIH HHS
- R56 HD093383 / NICHD NIH HHS
Conflict of Interest Statement
Conflict of interest None.
References
This article includes 6 references
- Battaglia DE, Goodwin P, Klein NA, Soules MR. Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women.. Hum Reprod 1996 Oct;11(10):2217-22.
- De La Fuente R. Chromatin modifications in the germinal vesicle (GV) of mammalian oocytes.. Dev Biol 2006 Apr 1;292(1):1-12.
- De La Fuente R, Viveiros MM, Wigglesworth K, Eppig JJ. ATRX, a member of the SNF2 family of helicase/ATPases, is required for chromosome alignment and meiotic spindle organization in metaphase II stage mouse oocytes.. Dev Biol 2004 Aug 1;272(1):1-14.
- Siddiqui MA, Gastal EL, Ju JC, Gastal MO, Beg MA, Ginther OJ. Nuclear configuration, spindle morphology and cytoskeletal organization of in vivo maturing horse oocytes.. Reprod Domest Anim 2009 Jun;44(3):435-40.
- Vanderwall DK, Hyde KJ, Woods GL. Effect of repeated transvaginal ultrasound-guided follicle aspiration on fertility in mares.. J Am Vet Med Assoc 2006 Jan 15;228(2):248-50.
- Vanderwall DK. Early embryonic loss in the mare. J. Equine Vet. Sci. 28, 691–702.
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