Characterizing the Serum Proteome of Donkeys (Equus asinus).
Abstract: Serum and plasma are commonly used in clinical practice considering the widely accepted fact that the "normal" protein expression pattern of a healthy animal changes under disease conditions. We herein used a label-free mass spectrometry-based quantitative proteomics approach to characterize the serum proteome of donkeys. A total of 277 unique proteins were identified from 2,388 unique peptides. Gene ontology analyses showed that the most frequent processes were related to metabolic activities and biological regulation, response to stimulus, and immune system processes. The main annotated areas of origin were the extracellular region, extracellular region part, and organelle, and their molecular functions included binding, catalytic activity, and molecular function regulator. Analyses using the Clusters of Orthologous Groups for Eukaryotic Complete Genomes database indicated that the identified proteins could be categorized into three main groups: signal transduction mechanisms, amino acid transport and metabolism, and defense mechanisms. Most of the unique proteins were associated with the complement and coagulation cascades, and they participated in several disease-related metabolic pathways. Our results should be crucial for further analyses of changes in different physiological and pathophysiological conditions in donkeys.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-06-20 PubMed ID: 32797796DOI: 10.1016/j.jevs.2020.103174Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article describes a scientific study aimed at characterizing the serum proteome of donkeys. Using mass spectrometry, the study identified 277 unique proteins in donkey’s serum and their potential role in different physiological and pathophysiological conditions.
Methodology
- The researchers used a label-free mass spectrometry-based quantitative proteomics approach to analyze the serum of donkeys. Mass spectrometry is a powerful tool that allows for the identification and quantification of proteins in a sample.
- They identified a total of 277 unique proteins from 2,388 unique peptides. These proteins were further categorized according to their functions and where they originate in the donkeys’ body.
Gene Ontology and Database Analyses
- The identified proteins were analyzed using gene ontology, a tool that provides specifics about the functions and locations of proteins in the body.
- It was found that most of the proteins were related to metabolic activities, biological regulation, response to stimulus, and immune system processes.
- According to the origin, the proteins were mostly found in the extracellular region, extracellular region part, and organelle.
- As per their molecular functions, the proteins were involved in binding, catalytic activity, and molecular function regulator.
- A database named Clusters of Orthologous Groups for Eukaryotic Complete Genomes was used to further categorize the identified proteins into three main groups: signal transduction mechanisms, amino acid transport and metabolism, and defense mechanisms.
Role of Identified Proteins
- The research suggested that most unique proteins were associated with the complement and coagulation cascades. The complement and coagulation cascades are important for a body’s immune response and blood clotting mechanism respectively.
- The mentioned proteins also played an important role in several disease-related metabolic pathways.
Implication of the Study
- The results could provide a basis for further studies examining changes in different physiological and pathophysiological conditions in donkeys.
- The findings could eventually help in disease diagnosis and treatment in donkeys.
Cite This Article
APA
Han Y, Tang C, Liao Q, Li Z, Deng L.
(2020).
Characterizing the Serum Proteome of Donkeys (Equus asinus).
J Equine Vet Sci, 92, 103174.
https://doi.org/10.1016/j.jevs.2020.103174 Publication
Researcher Affiliations
- Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province, PR China.
- Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province, PR China.
- Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province, PR China.
- Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province, PR China.
- Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province, PR China. Electronic address: ldeng@syau.edu.cn.
MeSH Terms
- Animals
- Equidae
- Gene Ontology
- Mass Spectrometry / veterinary
- Proteome / genetics
- Proteomics
Citations
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