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Home / Equine Research Bank / Inflammation / Chemiluminescence response of equine alveolar macrophages du...
Inflammation1983; 7(2); 169-182; doi: 10.1007/BF00917821

Chemiluminescence response of equine alveolar macrophages during stimulation with latex beads, or IgG-opsonized sheep red blood cells.

Abstract: Isolated equine alveolar macrophages were shown to generate a luminol-dependent light response when challenged with a phagocytic stimulus. The chemiluminescent response was not detected with luminol prepared at 1.0 x 10(-5) or 1.0 x 10(-4) molar concentrations, but was readily quantitated when used at a 1.0 x 10(-3) molar concentration. Challenge of the alveolar macrophages with latex particles or with equine IgG-coated sheep red blood cells elicited the luminol-dependent light response, whereas unchallenged equine alveolar macrophages or those challenged with unopsonized erythrocytes failed to emit light above background levels. Latex-bead-challenged macrophages released 8.06 times the total amount of light as those equine alveolar macrophages challenged with equine IgG-opsonized erythrocytes. This study represents the first investigation on chemiluminescence and equine alveolar macrophages.
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Publication Date: 1983-06-01 PubMed ID: 6862592DOI: 10.1007/BF00917821Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the light response of isolated horse lung cells when exposed to a phagocytic stimulus, providing groundbreaking insights on chemiluminescence and equine alveolar macrophages.

Chemiluminescent Response of Equine Alveolar Macrophages

  • This study investigates the light-emitting response (chemiluminescence) of equine alveolar macrophages (a type of immune cell found in the lungs of horses) when stimulated with certain substances.
  • These cells were isolated for the research and exposed to phagocytic stimulus, which promotes the process of phagocytosis, where cells engulf bacteria or other foreign substances to destroy them.
  • The research found that equine alveolar macrophages generated a light response that is dependent on luminol, a chemical that exhibits chemiluminescence, glowing blue when mixed with an appropriate oxidizing agent.

Exposure to Different Stimulation

  • The research involved testing the cells’ response with different molar concentrations of luminol, it was found that only 1.0 x 10(-3) molar concentration resulted in quantifiable light response. Lower concentrations did not lead to detectable chemiluminescence.
  • The study also involved challenging the alveolar macrophages with latex particles or erythrocytes (red blood cells) coated with equine IgG, a type of antibody. The aim was to see if these stimuli would elicit the luminol-dependent light response.
  • The cells did show a chemiluminescent response on challenge with both latex particles and IgG-coated erythrocytes. In contrast, unchallenged macrophages or those exposed to uncompromised erythrocytes did not produce light above background levels.

Comparative Light Emissions with Different Challenges

  • The study looked into the comparative light emissions of alveolar macrophages when challenged with latex beads or IgG-opsonized erythrocytes. The findings showed that the macrophages released 8.06 times more light when challenged with latex beads compared to when challenged with erythrocytes coated with equine IgG.
  • This indicates a greater chemiluminescent response when the macrophages deal with latex particles. However, the reasoning behind this outcome needs further exploration.

Significance of the Study

  • This research is the first of its kind to study chemiluminescence in equine alveolar macrophages.
  • The findings could have potential applications in the field of equine health, particularly related to immunological responses and potential diagnostic tools centering around chemiluminescence. Future studies can use these insights as a foundation to further understand the specific reactions of equine alveolar macrophages and their potential clinical implications.

Cite This Article

APA
Dyer RM, Leid RW. (1983). Chemiluminescence response of equine alveolar macrophages during stimulation with latex beads, or IgG-opsonized sheep red blood cells. Inflammation, 7(2), 169-182. https://doi.org/10.1007/BF00917821

Publication

Inflammation
ISSN: 0360-3997
NlmUniqueID: 7600105
Country: United States
Language: English
Volume: 7
Issue: 2
Pages: 169-182

Researcher Affiliations

Dyer, R M
    Leid, R W

      MeSH Terms

      • Animals
      • Cells, Cultured
      • Dose-Response Relationship, Drug
      • Erythrocytes / immunology
      • Horses
      • Immunoglobulin G / immunology
      • Latex / immunology
      • Luminescent Measurements
      • Luminol / pharmacology
      • Macrophage Activation
      • Macrophages / immunology
      • Opsonin Proteins / immunology
      • Pulmonary Alveoli / immunology
      • Sheep

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      Citations

      This article has been cited 2 times.
      1. Morton HC, Pleass RJ, Storset AK, Brandtzaeg P, Woof JM. Cloning and characterization of equine CD89 and identification of the CD89 gene in chimpanzees and rhesus macaques. Immunology 2005 May;115(1):74-84.
        doi: 10.1111/j.1365-2567.2005.02129.xpubmed: 15819699google scholar: lookup
      2. Ansoborlo E, Chalabreysse J, Hengé-Napoli MH, Pujol E. In vitro chemical and cellular tests applied to uranium trioxide with different hydration states. Environ Health Perspect 1992 Jul;97:139-43.
        doi: 10.1289/ehp.9297139pubmed: 1396449google scholar: lookup
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