Cholesterol Loaded Cyclodextrin Supplementation Enhances the Cholesterol-to-Phospholipid Ratio and Diminishes Oxidative Stress in Jack Spermatozoa During Cryopreservation.
- Journal Article
Summary
The research article focuses on the influence of cholesterol supplementation on semen quality and oxidative stress levels in jack (Martina franca) spermatozoa during cryopreservation. The study suggests that cholesterol supplementation reduces oxidative stress and enhances semen quality post-thaw by stabilizing sperm membranes and increasing the cholesterol-to-phospholipid ratio.
Research Methodology
The study was carried out on forty-eight ejaculates collected from six jack stallions. The researchers divided each fresh semen sample into five equal fractions after introducing a primary extender with varied levels of cholesterol-loaded cyclodextrin (CLC). The concentrations used were 0, 1, 1.5, 2, and 3 mg/mL CLC. Following adjustments to achieve a consistent spermatozoa concentration, the samples were cryopreserved using tailored freezing protocols.
Evaluation of Samples
- The researchers evaluated various seminal parameters, the cholesterol-to-phospholipid ratio, and the oxidative status of the jack spermatozoa at all stages of cryopreservation.
- Oxidative stress levels were assessed by measuring the levels of malondialdehyde, glutathione, and total antioxidant capacity.
Results
The results showed a significant increase in the mean percent values for seminal quality parameters and oxidative parameters in the CLC-treated groups, with the highest values observed at a CLC concentration of 2 mg per 120 × 10 spermatozoa.
Conclusion
The study concluded that introducing CLC supplementation before cryopreservation significantly reduced oxidative stress levels and increased the cholesterol-to-phospholipid ratio in the semen during the cryopreservation process. Additional findings include:
- Lowered lipid peroxidation levels
- Reduced damage to sperm plasma and acrosome membranes
- Improvement in the stability and integrity of post-thaw sperm
These results highlight the importance of cholesterol supplementation for maintaining semen quality during cryopreservation, potentially opening up new ways to enhance the efficiency of semen storage methods.
Cite This Article
Publication
Researcher Affiliations
- Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan, India.
- Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan, India.
- Animal Science Division, ICAR- Central Island Agricultural Research Institute, Port Blair, India.
- Equine Production Campus, ICAR-National Research Centre on Equines, Bikaner, Rajasthan, India.
- Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan, India.
- Equine Production Campus, ICAR-National Research Centre on Equines, Bikaner, Rajasthan, India.
- ICAR-National Research Centre on Equines, Hisar, Haryana, India.
- Equine Production Campus, ICAR-National Research Centre on Equines, Bikaner, Rajasthan, India. Electronic address: raotalluri79@gmail.com.
MeSH Terms
- Animals
- Cholesterol
- Cryopreservation / veterinary
- Cryoprotective Agents / pharmacology
- Cyclodextrins / pharmacology
- Dietary Supplements
- Horses
- Male
- Oxidative Stress
- Phospholipids
- Semen Analysis / veterinary
- Semen Preservation / veterinary
- Sperm Motility
- Spermatozoa
Citations
This article has been cited 4 times.- Gambini A, Smith JM, Gurkin RJ, Palacios PD. Current and Emerging Advanced Techniques for Breeding Donkeys and Mules. Animals (Basel) 2025 Mar 29;15(7).
- He M, Xu J, Sun L, Wu C, Zhang S, Gao J, Zhang D, Dai J. Optimization of Cholesterol-Loaded Cyclodextrin Combined with Soybean Lecithin as a Cryoprotectant for Rooster Sperm. Vet Sci 2024 Dec 13;11(12).
- Zhang S, Zhang H, Liu K, Xu X, Qin Y, Xiao L, Zhou C, Wu J, Liu Y, Bai J. Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation. Anim Biosci 2024 Sep;37(9):1558-1567.
- Ebel F, Ulloa O, Strobel P, Ramírez-Reveco A. Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere. Front Vet Sci 2021;8:662887.
