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Immunogenetics1988; 28(5); 362-364; doi: 10.1007/BF00364235

Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization.

Abstract: The first gene assignment to a horse chromosome is reported for equine leucocyte antigen (ELA), the major histocompatibility complex of the horse. A cloned DNA sequence derived from a class I gene of the porcine major histocompatibility complex was used as a probe for an in situ hybridization experiment. We present the regional localization of ELA, using this sequence, to equine chromosome 20q14-q22.
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Publication Date: 1988-01-01 PubMed ID: 3169882DOI: 10.1007/BF00364235Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study discusses the identification of the first gene, equine leucocyte antigen (ELA), situated on a horse chromosome, using a DNA sequence from a class I gene of the porcine major histocompatibility complex. This finding helps in understanding regional localization of ELA on the horse’s chromosome 20q14-q22.

Introduction and Methodology

  • The article discusses the first instance of gene assignment to a horse chromosome.
  • The gene studied is the equine leucocyte antigen (ELA), which plays a vital role in the immune system of the horse.
  • To conduct this genetic assignment, a cloned DNA sequence, derived from a class I gene of the porcine (pig) major histocompatibility complex, was utilized as a probe.
  • The use of this sequence allowed for an in situ hybridization experiment to occur, a method which helps identify specific sequences in a given chromosome, thereby giving the localization of a certain gene.

Findings

  • The focus of this experiment was the regional localization of the ELA, giving researchers insights into the gene’s exact location and potential function in the genome.
  • From their study, researchers localised the ELA gene to the equine chromosome 20q14-q22. This provides a greater understanding of where the ELA gene resides on the horse chromosome.

Implications and Significance

  • This thorough understanding of the localization of the ELA gene is beneficial for comprehensive studies on equine genetics, specifically concerning the horse’s immune system.
  • Identifying where genes reside in the chromosome is essential to further genetic research, as it enables easy identification of potential genetic abnormalities or genetic diseases.
  • The methodology used in the study, cloning a DNA sequence from a class I gene of the pig, can also be applied for gene mapping in other species, broadening overall genetic understanding.

Cite This Article

APA
Ansari HA, Hediger R, Fries R, Stranzinger G. (1988). Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization. Immunogenetics, 28(5), 362-364. https://doi.org/10.1007/BF00364235

Publication

Immunogenetics
ISSN: 0093-7711
NlmUniqueID: 0420404
Country: United States
Language: English
Volume: 28
Issue: 5
Pages: 362-364

Researcher Affiliations

Ansari, H A
  • Swiss Federal Institute of Technology, Institute of Animal Sciences, Zurich, Switzerland.
Hediger, R
    Fries, R
      Stranzinger, G

        MeSH Terms

        • Animals
        • Chromosome Mapping
        • DNA Probes
        • Histocompatibility Antigens Class I / immunology
        • Horses / genetics
        • Horses / immunology
        • Major Histocompatibility Complex
        • Nucleic Acid Hybridization

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