Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization.
Abstract: The first gene assignment to a horse chromosome is reported for equine leucocyte antigen (ELA), the major histocompatibility complex of the horse. A cloned DNA sequence derived from a class I gene of the porcine major histocompatibility complex was used as a probe for an in situ hybridization experiment. We present the regional localization of ELA, using this sequence, to equine chromosome 20q14-q22.
Publication Date: 1988-01-01 PubMed ID: 3169882DOI: 10.1007/BF00364235Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study discusses the identification of the first gene, equine leucocyte antigen (ELA), situated on a horse chromosome, using a DNA sequence from a class I gene of the porcine major histocompatibility complex. This finding helps in understanding regional localization of ELA on the horse’s chromosome 20q14-q22.
Introduction and Methodology
- The article discusses the first instance of gene assignment to a horse chromosome.
- The gene studied is the equine leucocyte antigen (ELA), which plays a vital role in the immune system of the horse.
- To conduct this genetic assignment, a cloned DNA sequence, derived from a class I gene of the porcine (pig) major histocompatibility complex, was utilized as a probe.
- The use of this sequence allowed for an in situ hybridization experiment to occur, a method which helps identify specific sequences in a given chromosome, thereby giving the localization of a certain gene.
Findings
- The focus of this experiment was the regional localization of the ELA, giving researchers insights into the gene’s exact location and potential function in the genome.
- From their study, researchers localised the ELA gene to the equine chromosome 20q14-q22. This provides a greater understanding of where the ELA gene resides on the horse chromosome.
Implications and Significance
- This thorough understanding of the localization of the ELA gene is beneficial for comprehensive studies on equine genetics, specifically concerning the horse’s immune system.
- Identifying where genes reside in the chromosome is essential to further genetic research, as it enables easy identification of potential genetic abnormalities or genetic diseases.
- The methodology used in the study, cloning a DNA sequence from a class I gene of the pig, can also be applied for gene mapping in other species, broadening overall genetic understanding.
Cite This Article
APA
Ansari HA, Hediger R, Fries R, Stranzinger G.
(1988).
Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization.
Immunogenetics, 28(5), 362-364.
https://doi.org/10.1007/BF00364235 Publication
Researcher Affiliations
- Swiss Federal Institute of Technology, Institute of Animal Sciences, Zurich, Switzerland.
MeSH Terms
- Animals
- Chromosome Mapping
- DNA Probes
- Histocompatibility Antigens Class I / immunology
- Horses / genetics
- Horses / immunology
- Major Histocompatibility Complex
- Nucleic Acid Hybridization
References
This article includes 13 references
- Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.. Anal Biochem 1983 Jul 1;132(1):6-13.
- Vaiman M, Chardon P, Cohen D. DNA polymorphism in the major histocompatibility complex of man and various farm animals.. Anim Genet 1986;17(2):113-33.
- Guerin G, Bertaud M, Chardon P, Geffrotin C, Vaiman M, Cohen D. Molecular genetic analysis of the major histocompatibility complex in an ELA typed horse family.. Anim Genet 1987;18(4):323-36.
- Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.. Anal Biochem 1984 Feb;137(1):266-7.
- Bailey E, Antczak DF, Bernoco D, Bull RW, Fister R, Guerin G, Lázary S, Matthews S, McClure J, Meyer J. Joint report of the Second International Workshop on Lymphocyte Alloantigens of the Horse, held 3-8 October 1982.. Anim Blood Groups Biochem Genet 1984;15(2):123-32.
- Singer DS, Camerini-Otero RD, Satz ML, Osborne B, Sachs D, Rudikoff S. Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.. Proc Natl Acad Sci U S A 1982 Mar;79(5):1403-7.
- Fries R, Hediger R, Stranzinger G. Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization.. Anim Genet 1986;17(4):287-94.
- Ford CE, Pollock DL, Gustavsson I. Proceedings of the First International Conference for the Standardisation of Banded Karyotypes of Domestic Animals. University of Reading Reading, England. 2nd-6th August 1976.. Hereditas 1980;92(1):145-62.
- Bull RW. Joint report of the First International Workshop on Lymphocyte Alloantigens of the Horse held 24-29 October 1981.. Anim Blood Groups Biochem Genet 1983;14(2):119-37.
- Alexander AJ, Bailey E, Woodward JG. Analysis of the equine lymphocyte antigen system by Southern blot hybridization.. Immunogenetics 1987;25(1):47-54.
- Lin CC, Draper PN, De Braekeleer M. High-resolution chromosomal localization of the beta-gene of the human beta-globin gene complex by in situ hybridization.. Cytogenet Cell Genet 1985;39(4):269-74.
- Rabin M, Fries R, Singer D, Ruddle FH. Assignment of the porcine major histocompatibility complex to chromosome 7 by in situ hybridization.. Cytogenet Cell Genet 1985;39(3):206-9.
- Antczak DF, Bailey E, Barger B, Guerin G, Lazary S, McClure J, Mottironi VD, Symons R, Templeton J, Varewyck H. Joint report of the Third International Workshop on Lymphocyte Alloantigens of the Horse, Kennett Square, Pennsylvania, 25-27 April 1984.. Anim Genet 1986;17(4):363-73.
Citations
This article has been cited 11 times.- Liu C, Lei H, Ran X, Wang J. Genetic variation and selection in the major histocompatibility complex Class II gene in the Guizhou pony.. PeerJ 2020;8:e9889.
- Viļuma A, Mikko S, Hahn D, Skow L, Andersson G, Bergström TF. Genomic structure of the horse major histocompatibility complex class II region resolved using PacBio long-read sequencing technology.. Sci Rep 2017 Mar 31;7:45518.
- Yao S, Qi J, Liu J, Chen R, Pan X, Li X, Gao F, Xia C. Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope.. Acta Crystallogr Sect F Struct Biol Cryst Commun 2012 Jan 1;68(Pt 1):20-3.
- Kamath PL, Getz WM. Adaptive molecular evolution of the Major Histocompatibility Complex genes, DRA and DQA, in the genus Equus.. BMC Evol Biol 2011 May 18;11:128.
- Sasaki M, Hasebe R, Makino Y, Suzuki T, Fukushi H, Okamoto M, Matsuda K, Taniyama H, Sawa H, Kimura T. Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D.. Genes Cells 2011 Apr;16(4):343-57.
- Tallmadge RL, Lear TL, Antczak DF. Genomic characterization of MHC class I genes of the horse.. Immunogenetics 2005 Nov;57(10):763-74.
- Lindgren G, Sandberg K, Persson H, Marklund S, Breen M, Sandgren B, Carlstén J, Ellegren H. A primary male autosomal linkage map of the horse genome.. Genome Res 1998 Sep;8(9):951-66.
- Bowling AT, Breen M, Chowdhary BP, Hirota K, Lear T, Millon LV, Ponce de Leon FA, Raudsepp T, Stranzinger G. International system for cytogenetic nomenclature of the domestic horse. Report of the Third International Committee for the Standardization of the domestic horse karyotype, Davis, CA, USA, 1996.. Chromosome Res 1997 Nov;5(7):433-43.
- Raudsepp T, Otte K, Rozell B, Chowdhary BP. FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11.. Mamm Genome 1997 Aug;8(8):569-72.
- Breen M, Lindgren G, Binns MM, Norman J, Irvin Z, Bell K, Sandberg K, Ellegren H. Genetical and physical assignments of equine microsatellites--first integration of anchored markers in horse genome mapping.. Mamm Genome 1997 Apr;8(4):267-73.
- Albright D, Bailey E, Woodward JG. Nucleotide sequence of a cDNA clone of the horse (Equus caballus) DRA gene.. Immunogenetics 1991;34(2):136-8.
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